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1.
The two gene-duplicated cAMP binding domains in the regulatory subunits of cAMP dependent protein kinase are each comprised of an A helix, an eight-stranded beta-barrel, and a B and C helix (1). The A domain is required for high affinity binding to C, while the B domain regulates access to the A domain. Using a combination of a yeast two-hybrid screen coupled with deletion analysis, cAMP binding domain A of RI was dissected into two structurally and functionally distinct subsites, one that binds cAMP and another that binds the C subunit. The minimum stable subdomain required for binding to C in the 1-3 micromolar range is composed of residues 94-169, while residues 236-244, mapped to the C helix of cAMP binding domain A, were defined as a second surface necessary for high affinity (5-10 nanomolar) binding to C. This portion of the C helix, due to its position directly between the two subsites, serves as a molecular switch for either a cAMP-bound conformation or a C-bound conformation and can thus modulate interactions of cAMP binding domain A with cAMP, with C, and with cAMP binding domain B. 相似文献
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BACKGROUND: An epidemic of bilateral optic neuropathy is affecting large numbers of people aged between 10 and 40 in Dar es Salaam, the capital city of Tanzania. The disease is characterised by acute onset of bilateral visual impairment, bilateral impairment of colour vision, and a characteristic temporal pallor of the optic discs. The disease often occurs in association with peripheral neuropathy and sensorineural hearing loss. This report presents the first data on disease prevalence in adolescents, based on a rapid assessment of schoolchildren. METHODS: Three schools in Dar es Salaam were visited and all children aged between 10 and 16 were screened for the disease. RESULTS: The prevalence of bilateral optic neuropathy among the schoolchildren is estimated to be 1.0% (95% CI 0.5-1.4%). This suggests that 5000 children (95% CI 2600-7300) aged 10-16 in Dar es Salaam may have the disease. CONCLUSION: The results of this rapid assessment indicate this epidemic is a major public health problem. The prevalence of the disease in the community is likely to be far higher than found in this survey because children with the disease may have withdrawn from school. As the disease predominantly affects young adults, resulting in impaired vision and hearing, the economic and social consequences are enormous. 相似文献
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Brant O 《Computers in healthcare》1990,11(9):26-28
As HMO enrollment climbs, managed care companies like Sacramento-based Foundation Health are turning to easily upgradable systems to give management and customers the information edge they need to thrive in the 1990s. 相似文献
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Covariance structures analysis is often used in nursing research to appraise statistical models reflecting complex human health processes. The model selection approach in covariance structures analysis is designed to select the "best" model from a specified set of theoretically defensible, competing alternatives, all of which are viewed as approximations. Model selection criteria explicitly incorporate both model misfit in the population and sampling error to evaluate the set of models. The result is that interpretability of model parameters and goodness-of-fit are enhanced simultaneously. Relative merits of the model selection approach are identified in light of technical concerns, parsimony, and use of scientific theory in nursing. 相似文献
9.
A theoretical analysis was carried out on the change of composition of a deposit obtained by the dual pulse method of forming laminar metal foils, with transition from a low current to a high current pulse, both in the galvanostatic and the potentiostatic mode of deposition. It was shown that the existence of a transition layer of varying composition between a layer of pure metal 1 and a layer consisting predominantly of the metal 2 is an inherent consequence of the electrochemical process, primarily because of an induction period in the concentration polarization with respect to ions of metal 1. The importance of this transition layer increases as the thickness of the layers of the two metals decreases. Eventually this limits the possibility of obtaining a sharp boundary between the layers, when the nanometre region of layer thickness is reached. Equations are given for calculating the deposition current density and rate of stirring of the electrolyte which provide a deposit of a required level of metal 1 in the layer of metal 2, as well as a required sharpness of the boundary between two layers. Experimental proof of the correctness of the analysis was sought. It was found that significant changes in the properties of the deposit occur in the same range of layer thickness in which the transition of the composition takes places.Nomenclature c,1, ac,2
transfer coefficient of the cathodic processes
-
C
interfacial capacitance
- C1, C2
concentration of the ions of metals 1 and 2 at the interface r
- C
0
1
, C
0
2
concentration of ions of the metals 1 and 2 in solution
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D
1
diffusion coefficient for the diffusion of ions of the metal 1
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E
r,1,E
r,2
reversible potentials of metals 1 and 2, respectively
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F
the Faraday constant
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J
0
1
,J
0
2
exchange current density of the metals 1 and 2, respectively
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M
1,M
2
atomic weights of the metals 1 and 2, respectively
-
kinematic viscosity of solution
- 1, 2
densities of the metals 1 and 2 respectively
-
rotation speed (r.p.s.)
-
z
number of electrons exchanged in the deposition process
This paper is dedicated to Professor Brian E. Conway on the occasion of his 65th birthday, and in recognition of his outstanding contribution to electrochemistry. 相似文献
10.
Oxidation of formic acid at a high surface area supported platinum modified by foreign metal adatoms
The anodic oxidation of formic acid was studied on platinum distributed in the form of small particles in a porous substrate modified by metal adatoms deposited at underpotentials. The rate of the reaction was greatly enhanced on the commercial catalyst Powercat 3000 (PC 3000). The hydrogen adsorption and the underpotential deposition of several metals at PC 3000 were shown to be dissimilar to that at smooth polycrystalline Pt. Steady-state and long-term experiments were performed to determine the stability of this electrode. 相似文献