Experimental Evidence for Seed Metabolic Allometry in Barrel Medic (Medicago truncatula Gaertn.)

Seed size is often considered to be an important trait for seed quality, i.e., vigour and germination performance. It is believed that seed size reflects the quantity of reserve material and thus the C and N sources available for post-germinative processes. However, mechanisms linking seed size and quality are poorly documented. In particular, specific metabolic changes when seed size varies are not well-known. To gain insight into this aspect, we examined seed size and composition across different accessions of barrel medic (Medicago truncatula Gaertn.) from the genetic core collection. We conducted multi-elemental analyses and isotope measurements, as well as exact mass GC–MS metabolomics. There was a systematic increase in N content (+0.17% N mg⁻¹) and a decrease in H content (–0.14% H mg⁻¹) with seed size, reflecting lower lipid and higher S-poor protein quantity. There was also a decrease in ²H natural abundance (δ²H), due to the lower prevalence of ²H-enriched lipid hydrogen atoms that underwent isotopic exchange with water during seed development. Metabolomics showed that seed size correlates with free amino acid and hexoses content, and anticorrelates with amino acid degradation products, disaccharides, malic acid and free fatty acids. All accessions followed the same trend, with insignificant differences in metabolic properties between them. Our results show that there is no general, proportional increase in metabolite pools with seed size. Seed size appears to be determined by metabolic balance (between sugar and amino acid degradation vs. utilisation for storage), which is in turn likely determined by phloem source metabolite delivery during seed development.     

Harmonical characterization of a microstrip bend via the finitedifference time domain method

A 90° microstrip bend is characterized using a time-frequency method based on the finite-difference-time-domain (FDTD) method. Time evolution of the currents generated by FDTD are Fourier transformed to lead to S-parameters and the radiated powers characteristic of the microstrip bend. The method for calculating both radiation and surface wave losses is developed for microstrip structures. The results of the 90° microstrip bend are compared with the results of the mitered microstrip bend     

A new method to quantify retention-failed cells of an EEPROM CAST

The cell array stress test (CAST) is a very simple tool to study one of the main issues of Non Volatile Memory reliability: data retention. However, it is not possible to easily quantify and localise the retention-failed cells of a CAST. Thus, a new experimental technique to localize and to quantify retention-failed EEPROM cells into a CAST is presented in this paper. This new technique is based on light emission microscopy; the aim is to observe light emission coming from cells and to localize their position with accuracy on CAST area. It is a visual and non destructive method which validity has been shown on cycled cells after a retention test.     

Direct Radiation Effects on the Structure and Stability of Collagen and Other Proteins

In this review, recent progress in understanding the direct effects of radiation on the structure and stability of collagen, the most abundant protein in the human body, and other proteins is surveyed. Special emphasis is placed on the triple-helical structure of collagen, as studied by means of collagen mimetic peptides. The emerging patterns are the dose dependence of radiation processes and their abundance, the crucial role of radicals in covalent-bond formation (crosslinking) or cleavage, and the influence of the radiation energy and nature. Future research should allow fundamental questions, such as charge transfer and fragmentation dynamics triggered by ionization, to be answered, as well as developing applications such as protein-based biomaterials, notably with properties controlled by irradiation.     

Automatic evaluation of the Valsalva sinuses from cine-MRI

Object   

Although, there is no global consensus on their measurement, magnetic resonance imaging (MRI) appears to be particularly attractive for the study of the sinuses of Valsalva (SV). The purpose of this study was to automatically evaluate the SV from cine-MRI using a standardized method.     

Modelling the effect of temperature on crack onset strain of brittle coatings on polymer substrates

The effect of temperature on the crack onset strain (COS) of brittle coatings on polymer substrates was investigated through a series of temperature-controlled tensile tests carried out in situ under an optical microscope. It was observed that the failure of such materials under tensile strain was strongly affected by temperature, but the exact behaviour was heavily dependent upon the type of material used. Below the glass transition temperature, T_g, of the polymer substrate, an increase in temperature led to a decrease in crack onset strain. Above the T_g, the substrate softening effects and corresponding shrinkage behaviour had a presiding role, leading to an increase in COS at elevated temperatures. The experimental COS data were modelled as a linear superposition of an intrinsic COS and the internal strain taking into consideration the respective influences of temperature dependent energy release rate for crack propagation and thermal expansion behaviour. Using adjustable values of the coefficient of thermal expansion and toughness of the coating, the model was found to accurately reproduce the change of COS with temperature of two different coatings on aromatic polyester substrates. The proposed approach enables, for any thin film composite with known material properties, the COS at any given temperature to be predicted.     

Mechanical integrity of thin inorganic coatings on polymer substrates under quasi-static, thermal and fatigue loadings

The interplay between residual stress state, cohesive and adhesive properties of coatings on substrates is reviewed in this article. Attention is paid to thin inorganic coatings on polymers, characterized by a very high hygro-thermo-mechanical contrast between the brittle and stiff coating and the compliant and soft substrate. An approach to determine the intrinsic, thermal and hygroscopic contributions to the coating residual stress is detailed. The critical strain for coating failure, coating toughness and coating/substrate interface shear strength are derived from the analysis of progressive coating cracking under strain. Electro-fragmentation and electro-fatigue tests in situ in a microscope are described. These methods enable reproducing the thermo-mechanical loads present during processing and service life, hence identifying and modeling the critical conditions for failure. Several case studies relevant to food and pharmaceutical packaging, flexible electronics and thin film photovoltaic devices are discussed to illustrate the benefits and limits of the present methods and models.     

Retrospective memory bias for the frequency of potentially traumatic events: A prospective study.

We conducted a prospective study that tracked the frequency of potentially traumatic events (PTEs) and nontraumatic events among college students over a 4-year period using a weekly web-based survey. At the study's completion, participants attempted to recall the number of events they had endorsed on the web surveys. Although participants underrecalled the frequency of all types of life events, recollection was more accurate for PTEs than for non-PTEs. Recalled-frequency of PTEs was associated positively with distress at recall and inversely with trait self-enhancement. These effects were qualified by a distress × self-enhancement interaction. High distress at recall was associated with a greater recalled-frequency of PTEs, but only for people low in trait self-enhancement. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Canadian counselling psychology: From defining moments to ways forward.

Counselling psychology in Canada is a distinct and vibrant professional area representing the contributions of many people and events within a unique context. This article elaborates a number of defining moments or actions that reveal the distinct nature of this professional area through a recursive and interactive process with the social context. A number of ways forward are proposed as specific professional developments, as well as the resolution of a number of substantive issues identified within the recently adopted definition of counselling psychology. Moving forward as a discipline and a practice will actualize the potential of counselling psychology as both world and life enhancing. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

A large polymorphic repeat in the pericentromeric region of human chromosome 15q contains three partial gene duplications

We report the identification of a partial duplication of GABRA5 , a gene within the imprinted 15q11-q13 region. The duplicated locus maps to the pericentromeric region of 15q, proximal to the large deletions associated with Angelman and Prader-Willi syndromes. We also observed variation in the number of copies of this locus in different individuals, indicating that the duplication is part of a variable repeat. Investigation of the duplication in individuals with a normal karyotype revealed between one and four copies of the repeat on each chromosome 15, whereas from eight to 20 copies were found in individuals possessing a cytogenetically detectable elongation of the 15q region. The variable region is roughly 1 Mb in size and contains two other non-processed duplications, the immunoglobulin heavy chain (IgH) D segment gene and the neurofibromatosis type 1 (NF1) gene. One unit of the pericentromeric repeat is thus composed of duplications of genes from different chromosomal regions. Moreover, we have found replication asynchrony across the GABRA5 duplication, suggesting for the first time that the imprinted part of chromosome 15q extends proximal of the region commonly deleted in Angelman and Prader-Willi syndromes.