Supplementing with L-Tryptophan Increases Medium Protein and Alters Expression of Genes and Proteins Involved in Milk Protein Synthesis and Energy Metabolism in Bovine Mammary Cells

The objective of this study was to investigate the effects of supplementing with L-tryptophan (L-Trp) on milk protein synthesis using an immortalized bovine mammary epithelial (MAC-T) cell line. Cells were treated with 0, 0.3, 0.6, 0.9, 1.2, and 1.5 mM of supplemental L-Trp, and the most efficient time for protein synthesis was determined by measuring cell, medium, and total protein at 0, 24, 48, 72, and 96 h. Time and dose tests showed that the 48 h incubation time and a 0.9 mM dose of L-Trp were the optimal values. The mechanism of milk protein synthesis was elucidated through proteomic analysis to identify the metabolic pathway involved. When L-Trp was supplemented, extracellular protein (medium protein) reached its peak at 48 h, whereas intracellular cell protein reached its peak at 96 h with all L-Trp doses. β-casein mRNA gene expression and genes related to milk protein synthesis, such as mammalian target of rapamycin (mTOR) and ribosomal protein 6 (RPS6) genes, were also stimulated (p < 0.05). Overall, there were 51 upregulated and 59 downregulated proteins, many of which are involved in protein synthesis. The results of protein pathway analysis showed that L-Trp stimulated glycolysis, the pentose phosphate pathway, and ATP synthesis, which are pathways involved in energy metabolism. Together, these results demonstrate that L-Trp supplementation, particularly at 0.9 mM, is an effective stimulus in β-casein synthesis by stimulating genes, proteins, and pathways related to protein and energy metabolism.     

A penalized batch-Bayesian approach to informative path planning for decentralized swarm robotic search

Autonomous Robots - Swarm-robotic approaches to search and target localization, where target sources emit a spatially varying signal, promise unparalleled time efficiency and robustness. With most...     

Thermodynamic evaluation of integrated gasification combined cycle: Comparison between high‐ash and low‐ash coals

In the present study, a coal‐integrated gasification combined cycle power plant is simulated. A high‐ash coal and low‐ash coal are considered to compare the performance of the plant. The combined cycle is in typical commercial size with 450 MW capacity. The feeds are Tabas and Illinois #6 coals which approximately contain more than 30% and 10% ash and have higher heating values of 22.7 MJ/kg and 26.8 MJ/kg, respectively. Energy and exergy analyses are done by aspen plus ^® and ees , respectively. Energy analysis shows that the overall efficiencies of power plants using high‐ash and low‐ash coals are 33% and 28%, respectively. The result shows that in high‐ash case, 52 kg/s coal, 10 kg/s water, and 1050 kg/s air and in low‐ash case, 48 kg/s coal and 820 kg/s air are required for providing mentioned power, approximately. Exergy analysis shows that maximum exergy destruction is in heat recovery steam generator unit. Investigating the emissions shows that high percent of ash in the coal composition has slight effects on the IGCC pollution. Finally, from thermodynamic viewpoint, it is concluded that the high‐ash coal, like the conventional one, can be used as thermally efficient and environmentally compatible feed of IGCCs. Copyright © 2016 John Wiley & Sons, Ltd.     

Application of multivariate statistical analyses in the differentiation between two phosphate ores from the Nuba Mountains,Sudan

In previously published research, uranium abundance and major and trace element content were determined in phosphate ore samples from the Kurun and Uro areas of the Nuba Mountains in Sudan. The current work aims to characterise and differentiate between the phosphate ores from these two areas by subjecting the obtained geochemical data to quantitative and qualitative statistical analyses. Multivariate statistical analyses and correlation studies show several geochemical differences in the occurrence of uranium in Kurun and Uro phosphate ores. Uranium in Uro phosphate ore shows a higher degree of mobility coefficient of variability (CV%?=?91) than that in Kurun phosphate ore (CV%?=?48). Kurun and Uro phosphate ores are found to be good sources of titanium and strontium. The average concentrations of Ti and Sr in Uro phosphate ore are 4880 and 2780?mg?kg^–1, respectively. The corresponding values in Kurun phosphate ore are 7300 and 2630?mg?kg^–1, respectively. A cluster analysis of trace elements demonstrates that both Ti and Sr, which are the most abundant trace elements, appear in individual groups or in sub-groups. Uranium behaves in the same manner in both phosphate ores in that it associates in groups containing all the transition metals except for the most abundant trace element, titanium.     

Microscopic and macroscopic atomization characteristics of a pressure-swirl atomizer,injecting a viscous fuel oil

Combustion of heavy fuels is one of the main sources of greenhouse gases, particulate emissions, ashes, NOxand SOx. Gasification is an advanced and environmentally friendly process that generates combustible and clean gas products such as hydrogen. Some entrained flow gasifiers operate with Heavy Fuel Oil(HFO) feedstock. In this application, HFO atomization is very important in determining the performance and efficiency of the gasifiers.The atomization characteristics of HFO(Mazut) discharging from a pressure-swirl atomizer(PSA) are studied for different pressures difference(Δp) and temperatures in the atmospheric ambient. The investigated parameters include atomizer mass flow rate( _m), discharge coefficient(CD), spray cone angle(θ), breakup length(Lb), the unstable wavelength of undulations on the liquid sheet(λs), global and local SMD(sauter mean diameter) and size distribution of droplets. The characteristics of Mazut sheet breakup are deduced from the shadowgraph technique. The experiments on Mazut film breakup were compared with the predictions obtained from the liquid film breakup model. Validity of the theory for predicting maximum unstable wavelength was investigated for HFO(as a highly viscous liquid). A modification on the formulation of maximum unstable wavelength was presented for HFO. SMD decreases by getting far from the atomizer. The measurement for SMD and θ were compared with the available correlations. The comparisons of the available correlations with the measurements of SMD andθ show a good agreement for Ballester and Varde correlations, respectively. The results show that the experimental sizing data could be presented by Rosin-Rammler distributions very well at different pressure difference and temperatures.     

Amine corrosion and amine cracking of API 5L X52 carbon steel in the presence of hydrogen sulphide and carbon dioxide

In this research, stress corrosion cracking (SCC) and corrosion behaviour of API 5L X52 carbon steel in 25?wt-% diethanolamine solution, saturated/unsaturated with carbon dioxide and containing 0 and 200?ppm hydrogen sulphide at different temperatures were investigated using slow strain rate test, electrochemical measurement and microscopic analysis. In addition, the presence of heat stable amine salts (HSASs) in the test solution was studied using spectrophotometry and Fourier transform infra-red spectroscopy. Analysis of the results showed that the primary components to form HSASs exist in the solution. The results indicated that SCC is more likely in solutions without amine. Increase in corrosion rate of carbon steel by increase in temperature was clearly observed and concluded that the simultaneous presence of hydrogen sulphide and carbon dioxide in the solution can increase the corrosion rate of carbon steel more than having one of the gases in the solution.     

Porothermoelastic Analysis of the Response of a Stationary Crack Using the Displacement Discontinuity Method

Thermally induced volumetric changes in rock result in pore pressure variations, and lead to a coupling between the thermal and poromechanical processes. This paper examines the response of a fracture in porothermoelastic rock when subjected to stress, pore pressure, and temperature perturbations. The contribution of each mechanism to the temporal variation of fracture opening is studied to elucidate its effect. This is achieved by development and use of a transient displacement discontinuity (DD) boundary element method for porothermoelasticity. While the full range of the crack opening due to the applied loads is investigated with the porothermoelastic DD, the asymptotic crack opening is ascertained analytically. Good agreement is observed between the numerical and analytical calculations. The results of the study show that, as expected, an applied stress causes the fracture to open while a pore pressure loading reduces the fracture width (aperture). In contrast to the pore pressure effect, cooling of the crack surfaces increases the fracture aperture. It is found that the impact of cooling can be more significant when compared to that of hydraulic loading (i.e., an applied stress and pore pressure) and can cause significant permeability enhancement, particularly for injection/extraction operations that are carried out over a long period of time in geothermal reservoirs.     

Failure Analysis of AISI 321 Austenitic Stainless Steel Water Piping in a Power Plant

The objective of this work was to analyze a reported pitting damage mechanism in water piping of a power plant. The investigated material was a longitudinal-seam welded pipe which was made of AISI 321 austenitic stainless steel. Pitting occurred on the internal surfaces of the pipe and adjacent to the girth welds. Optical emission spectrometry, metallography, and electrochemical testing were the techniques employed to analyze the failure. The investigation showed that the flowing water contained chloride ions in which AISI 321 austenitic stainless steel is not immune against pitting corrosion. In addition, the polarization data showed the susceptibility of the material to pitting corrosion in the service conditions. Furthermore, the result of double loop electrochemical potentiokinetic reactivation tests showed that the material was sensitized during welding. It was found that the damage was due to pitting corrosion in chloride-containing water. Thus, it was recommended to replace the pipe with material having higher pitting resistance equivalent number to be more resistant against pitting corrosion.     

Thermal Energy Storage Module Design Using Energy and Exergy Analysis

In this paper, irreversibility of a thermal energy storage system is numerically investigated. The system consists of two concentric cylinders. The outer cylinder is filled with phase change material (PCM), while working fluid flows inside the inner pipe. The system works periodically. The related governing equations are solved by a control volume-based finite difference method. The effects of different parameters such as PCM size and melting point temperature are examined on the irreversibility of the system. The results show that the irreversibility of thermal storage module is strongly affected by the size of PCM (diameter and length of the external cylinder) and melting temperature. Based on the obtained results, the irreversibility of the system can be reduced by proper selection of PCM size and melting temperature.