全文获取类型
收费全文 | 915篇 |
免费 | 9篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 4篇 |
化学工业 | 41篇 |
金属工艺 | 5篇 |
机械仪表 | 6篇 |
建筑科学 | 18篇 |
矿业工程 | 4篇 |
能源动力 | 3篇 |
轻工业 | 66篇 |
水利工程 | 3篇 |
无线电 | 14篇 |
一般工业技术 | 39篇 |
冶金工业 | 686篇 |
原子能技术 | 1篇 |
自动化技术 | 35篇 |
出版年
2021年 | 3篇 |
2019年 | 4篇 |
2017年 | 4篇 |
2016年 | 6篇 |
2015年 | 5篇 |
2014年 | 7篇 |
2013年 | 16篇 |
2012年 | 5篇 |
2011年 | 9篇 |
2010年 | 11篇 |
2009年 | 6篇 |
2008年 | 7篇 |
2007年 | 10篇 |
2006年 | 7篇 |
2005年 | 5篇 |
2004年 | 5篇 |
2003年 | 3篇 |
2002年 | 11篇 |
2001年 | 9篇 |
2000年 | 6篇 |
1999年 | 15篇 |
1998年 | 185篇 |
1997年 | 108篇 |
1996年 | 85篇 |
1995年 | 51篇 |
1994年 | 35篇 |
1993年 | 36篇 |
1992年 | 11篇 |
1991年 | 6篇 |
1990年 | 5篇 |
1989年 | 8篇 |
1988年 | 9篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 9篇 |
1982年 | 9篇 |
1981年 | 8篇 |
1980年 | 14篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1977年 | 33篇 |
1976年 | 86篇 |
1975年 | 5篇 |
1974年 | 5篇 |
1973年 | 4篇 |
1971年 | 4篇 |
1965年 | 3篇 |
1964年 | 4篇 |
排序方式: 共有925条查询结果,搜索用时 31 毫秒
1.
The metabolism of the poly(A) tail is a process important for the translational regulation of maternal mRNAs in Xenopus laevis oocytes and early embryos. Two poly(A) nuclease (PAN) activities have been described in Xenopus embryo or activated egg extracts (Legagneux et al (1995) RNA 1, 1001-1008). These activities (default PAN and EgPAN) are distinguishable by their deadenylation kinetics and their substrate specificities. In this report, we show that these activities display different sensitivities to biochemical treatments. Urea and, to a lesser extent, spermidine, inhibit EgPAN at concentrations which have no effect on default PAN. Heparin activates default PAN but inhibits EgPAN. When extracts are fractionated by ultracentrifugation, the default activity is recovered in one unique fraction, whereas two fractions must be combined to reconstitute the EgPAN activity. Moreover, these two deadenylation activities are separable by size exclusion chromatography under native conditions. We conclude that these two deadenylation activities are mediated by two protein complexes. 相似文献
2.
3.
DH Kang CL Coe DO McCarthy NN Jarjour EA Kelly RR Rodriguez WW Busse 《Canadian Metallurgical Quarterly》1997,17(8):481-487
T cell cytokines play an important role in mediating airway inflammation in asthma. The predominance of a Th2 cytokine profile, particularly interleukin (IL)-4 and IL-5, is associated with the pathogenesis and course of asthma. The aim of this study was to test the hypothesis that a stressful life event alters the pattern of cytokine release in asthmatic individuals. Thirteen healthy controls and 21 asthmatic adolescents gave blood samples three times over a semester: midsemester, during the week of final examinations, and 2-3 weeks after examinations. Interferon-gamma (IFN-gamma), IL-2, IL-4, and IL-5 were measured from supernatants of cells stimulated with PHA/PMA for 24 h. Cells from asthmatic subjects released significantly more IL-5 during the examination and postexamination periods, whereas cells from healthy controls released significantly more IL-2 during the midsemester and examination periods, thereby indicating a bias for a Th2-like pattern in asthmatics and a Th1-like pattern in healthy controls. IL-4 and IL-5 production showed a marked decrease during and after examinations in healthy controls, whereas this decline was absent in asthmatics. The ratios of IFN-gamma:IL-4 and IFN-gamma:IL-5 also revealed significant changes in the profile of cytokine release across the semester. These results indicate differential cytokine responses in asthmatics that may become pronounced during periods of cellular activation. 相似文献
4.
Joseph A Wehrmeyer Zhongxian ChengDavid M Mosbacher Robert W PitzRobin Osborne 《Combustion and Flame》2002,128(3):232-241
Several opposed jet flames, produced by a lean H2-air jet opposing a rich or lean C3H8-air jet, are investigated. Spontaneous Raman spectroscopy is used for major species concentration and temperature measurements along the opposed jet centerline. The hot products of the H2-air flame simulate the burnt gases of strong-burning near-stoichiometric reactants as they impinge upon a weak-burning lean or rich hydrocarbon-fueled reactant mix, a situation encountered in stratified charge operation of direct injection spark ignition engines. In addition the H2-air flame hot products facilitate experimental data interpretation through the absence of carbon-bearing species. Good agreement between numerical and experimental data are obtained for a rich (equivalence ratio, φ = 1.25) C3H8-air jet versus a lean (φ = 0.4) H2-air jet. Two lean C3H8-air jets (φ = 0.64 or 0.60), versus the φ = 0.4 H2-air jet, are also investigated. For both of these flames, the amount of CO2 production strongly depends upon φ, with the φ = 0.64 flame having a peak CO2 mole fraction an order of magnitude higher than for the φ = 0.60 flame, and the C3H8 flames burning either as a normal flame (high CO2) or as a “negative flame speed” flame producing little CO2 and then only through diffusion of C3H8 into the hot products jet. The numerically predicted and experimental CO2 profiles agree well for the positive flame speed flame, but the large discrepancy between predicted and measured peak CO2 in the negative flame speed flame suggests modeling improvements are needed for this type of flame. 相似文献
5.
6.
NW Tomkins NN Jonsson MP Young AN Gordon KA McColl 《Canadian Metallurgical Quarterly》1997,75(10):722-723
On the basis of clinical signs and histological findings eight 9-month-old male rusa deer (Cervus timorensis) were diagnosed with sheep associated-malignant catarrhal fever. Following a variable course involving rectal temperatures around 40.5 degrees C, depression, inappetence, diarrhoea, corneal opacity and hypopyon all animals died or were euthanased over a 5-week period. Severe multifocal vasculitis, mainly periglomerular and in the arcuate vessels were consistent histological findings which in the past have been adequate to confirm clinical diagnosis of sheep associated-malignant catarrhal fever. A nested polymerase chain reaction test has been used to detect a sheep associated-malignant catarrhal fever PRC product, 238 base-pairs in size, in DNA extracted from lymphocyte preparations. The result supported the diagnosis of sheep associated-malignant catarrhal fever in these deer. 相似文献
7.
8.
Vladimir Kulyukin Chaitanya Gharpure John Nicholson Grayson Osborne 《Autonomous Robots》2006,21(1):29-41
We present a robot-assisted wayfinding system for the visually impaired in structured indoor environments. The system consists
of a mobile robotic guide and small passive RFID sensors embedded in the environment. The system is intended for use in indoor
environments, such as office buildings, supermarkets and airports. We describe how the system was deployed in two indoor environments
and evaluated by visually impaired participants in a series of pilot experiments. We analyze the system’s successes and failures
and outline our plans for future research and development. 相似文献
9.
Prednisolone therapy of idiopathic feline lower urinary tract disease: a double-blind clinical study
CA Osborne JM Kruger JP Lulich GR Johnston DJ Polzin LK Ulrich J Sanna 《Canadian Metallurgical Quarterly》1996,26(3):563-569
A double-blind clinical study was performed to evaluate prednisolone as treatment for idiopathic feline lower urinary tract disease. No differences in response were observed in prednisolone- and placebo-treated cats. 相似文献
10.
NV Zheleznova SL Mukomolov AB Zhebrun FS Noskov II Malkova EP Shargorodskaia AD Al'tshte?n VA Pliutnikova IN Lavrent'eva NN Ptichnikova 《Canadian Metallurgical Quarterly》1996,(5):49-53
The use of a recombinant poxvirus (RPV) strain, expressing HBsAg in the process of reproduction in different bioreactor systems under stationary and bioreactor conditions of cultivation, made it possible to obtain highly purified HBsAg. The identity and purity of HBsAg was confirmed by the analysis of its amino acid composition, SDS electrophoresis in polyacrylamide gel, electron microscopy and high-performance liquid chromatography. Good prospects of the use of RPV-expressed gene engineering HBsAg as the basis vaccines against hepatitis B was demonstrated in 10 experimental batches of vaccine. All batches of the preparation had pronounced immunogenicity and were safe and nontoxic in animal experiments. The ID50 of experimental batches did not exceed 211 ng/ml, which, according to the data of comparative experiments, was lower than, or equal to, corresponding values of analogous foreign commercial preparations, based on plasma or yeast HBsAg. 相似文献