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RGS proteins (regulators of G protein signaling) are GTPase activating proteins (GAPs) for Gi and Gq families of heterotrimeric G proteins but have not been found to interact with Gs alpha. The Gs alpha residue Asp229 has been suggested to be responsible for the inability of RGS proteins to interact with Gs alpha [Natochin, M., and Artemyev, N. O. (1998) J. Biol. Chem. 273, 4300-4303]. To test this hypothesis, we have investigated the possibility of generating an interaction between Gs alpha and RGS proteins by substituting Gs alpha Asp229 with Ser and replacing the potential Gs alpha Asp229 contact residues in RGS16, Glu129 and Asn131, by Ala and Ser, respectively. RGS16 and its mutants failed to interact with Gs alpha. A single mutation of Gs alpha, Asp229Ser, rendered the Gs alpha subunit with the ability to interact with RGS16 and RGS4. Like RGS protein binding to Gi and Gq alpha-subunits, RGS16 preferentially recognized the AlF4--bound conformation of Gs alpha Asp229Ser. In a single-turnover assay, RGS16 maximally stimulated GTPase activity of Gs alpha Asp229Ser by approximately 5-fold with an EC50 value of 7.5 microM. Our findings demonstrate that Asp229 of Gs alpha represents a major barrier for Gs alpha interaction with known RGS proteins. 相似文献
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安徽省第一建筑工程公司技术质量处 《建筑施工》2006,28(1):44-45
为降低建筑采暖和空调制冷的能耗,贵都花园外墙采用外保温,介绍了墙体外保温用材、施工程序及操作工艺,竣工后通过热工性能检测,取得了明显的节能效果,并获安徽省“省级科技示范工程”称号。 相似文献
5.
文章采用对乳化炸药水相析晶点的测定方法,研究了制备乳化炸药的几种水相析晶点对其稳定性的影响t最后得出了相应的结论。结果表明乳化炸药水相析晶点越低,乳化炸药越稳定,基质水溶值也越小;经16次高低温循环,乳化炸药的殉爆距离仍达到20mm。 相似文献
6.
对连续过程工业实施CIMS总体技术方案作了较为深入的探讨,并对天津市轧三制钢有限公司实施CIMS总体方案及技术路线进行了详细介绍。 相似文献
7.
CHEOL LAE ROH MOON NO LEE MYUNG JIN CHUNG 《International journal of systems science》2013,44(4):419-424
In this paper we propose a design method of an iterative learning controller (ILC) for a non-minimum phase (NMP) system by model-matching theory. The ILC consists of two learning filters acting on both the previous input signal and the previous error signal. To design the learning filters, we convert the convergence condition of the ILC into the model-matching problem and get the stable and proper learning filter by solving the Nevanlinna's algorithm. To show the usefulness of the proposed algorithm, some design examples are included. 相似文献
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本文针对海湾战争后的新形势,介绍了战略防御的新构想、战术防空导弹系统及21世纪的防空概念,论述了AEW、AWACS、战场监视目标攻击雷达及其它雷达的最新发展情况,此外,还对国外的反隐身技术进展情况作了介绍。 相似文献
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采用质谱分析等方法,测试了夏枚子金矿床石英流体包裹体和金属硫化物的同位素组成,获得石英流体包裹体Rb-Sr等时线年龄为105.4±7.2Ma;锶同位素初始比值为0.7126±0.0001;氢同位素组成δD‰为-91.7~-73.1;氧同位素组成δ~(18)OH_2O‰为5.68~6.23;金属硫化物的硫同位素组成δ~(34)S‰=-23.38~-14.52;铅同位素组成~(206)Pb/~(204)Pb=16.441~16.615,~(207)Pb/~(204)Pb=15.228~15.256,~(208)Pb/~(204)Pb=36.268~36.356。矿床成矿作用发生于燕山晚期,成矿热液主要为岩浆热液,金矿成矿物质来源于硅铝壳中下部太古代结晶基底,矿床成因是与燕山期岩浆热液有关的中—低温热液脉状金矿床。 相似文献
10.
CHANG JUNG KIM DAE SUNG YOON ZHONG-TAO JIANG KWANGSOO NO 《Journal of Materials Science》1997,32(5):1213-1219
The crystal orientations of lead zirconate titanate (PZT) thin films have been investigated by using various drying temperatures
in the sol–gel process. The films were dried at different temperatures between 310 and 350°C for pyrolysis and then were heat
treated at 650°C using rapid thermal annealing (RTA). TG/DTA and FTIR spectroscopy were used to detect the remnants of organic
materials in the thin films prior to the final heat treatment. In order to examine the relationship between the film orientation
and the remaining organic materials for the prior and final heat treatment, the films were fabricated with different coating
cycles and dried for different holding times and then annealed at 650°C. The preferred orientations were investigated using
X-ray diffraction.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献