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Proteinase B precursors are modified by an N-linked carbohydrate side chain at Asn 314. Glycosylation at this position is not required for proper localization, processing, or activation of the enzyme.  相似文献   
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Used semantic-priming procedures to examine limitations in the use of semantic context by 18 patients (mean age 68.9 yrs) with Alzheimer's disease (AD) and to determine whether any such contextual effects were mediated solely through automatic processes or whether attentional processes were also involved. Three tasks were applied to examine the effect of semantic context on the performance of 18 normal elderly Ss (mean age 67.2 yrs), 18 normal young Ss (mean age 24.1 yrs), and the AD Ss. When normal and AD Ss were asked to decide whether a given item was a member of a certain category, their response times were equally affected by the item's dominance in the category. The time that AD Ss took to recognize a word was actually affected more by the semantic context provided by a priming sentence than was that of normal Ss. When asked to generate the final word of an incomplete sentence, AD Ss performed very poorly unless potential responses were highly constrained by sentence context. (31 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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We hypothesized that older volunteers allocate more attentional resources to memory maintenance than do younger volunteers. Allocation of a resource supporting memory maintenance was inferred from performance and cardiovascular measures. Eighteen 18- to 26-yr-old men and eighteen 60- to 79-yr-old men performed a serial memory task both as a single task and as a dual task that added simple reaction time stimuli. Items presented early or later in the serial list created relatively low or high memory load. The results suggested that older men allocated greater attention to memory maintenance, particularly during high-memory-load items. They showed a slowing of dual-task reaction time and increased heart rate during high- vs. low-memory-load items. Cardiac and vascular reactions further suggested that memory maintenance is supported by phasic autonomic adjustments and that with age, more of this support is required for adequate maintenance of episodic memory. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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Low voltage electron microscopes working in transmission mode, like LVEM5 (Delong Instruments, Czech Republic) working at accelerating voltage 5 kV or scanning electron microscope working in transmission mode with accelerating voltage below 1 kV, require ultrathin sections with the thickness below 20 nm. Decreasing of the primary electron energy leads to enhancement of image contrast, which is especially useful in the case of biological samples composed of elements with low atomic numbers. As a result treatments with heavy metals, like post‐fixation with osmium tetroxide or ultrathin section staining, can by omitted. The disadvantage is reduced penetration ability of incident electrons influencing the usable thickness of the specimen resulting in the need of ultrathin sections of under 20 nm thickness. In this study we want to answer basic questions concerning the cutting of extremely ultrathin sections: Is it possible routinely and reproducibly to cut extremely thin sections of biological specimens embedded in commonly used resins with contemporary ultramicrotome techniques and under what conditions? Microsc. Res. Tech. 79:512–517, 2016. © 2016 Wiley Periodicals, Inc.  相似文献   
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The aim of this work was to examine the effect of a different dry matter (DM) contents (35 and 45% wt/wt) and fat in DM contents (40 and 50% wt/wt) on the textural and viscoelastic properties and microstructure of model processed cheeses made from real ingredients regularly used in the dairy industry. A constant DM content and constant fat in DM content were kept throughout the whole study. Apart from the basic chemical parameters, textural and viscoelastic properties of the model samples were measured and scanning electron microscopy was carried out. With increasing DM content, the rigidity of the products increased and the size of the fat globules in the model samples of the processed cheeses decreased. With increasing fat in DM content, the rigidity of the processed cheeses decreased and the size of the fat globules increased.  相似文献   
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Patients with Alzheimer's disease have been suggested to have a semantic memory impairment not present in the normal old. This article reviews the performance of Alzheimer patients on tests of various aspects of semantic memory, including word finding, knowledge of the semantic attributes, and associates of concepts, as well as their category membership. The effect that semantic context has on cognitive processes such as lexical and semantic priming and memory encoding is also reviewed. Finally, the ability of theoretical constructs such as implicit memory and automaticity to explain intertask variability in Alzheimer patients' semantic performance is discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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OBJECTIVE: The authors' goal was to compare serum anticholinergicity of 61 elderly depressed patients randomly assigned to double-blind treatment with paroxetine (N=31) or nortriptyline (N=30). METHOD: Both antidepressants were titrated in a standardized manner, and plasma was sampled weekly for measurement of paroxetine and nortriptyline and its hydroxy metabolite concentrations. Serum anticholinergicity was measured at baseline and after 1, 4, and 6 weeks of treatment. Side effects were assessed by using a validated scale. RESULTS: After correcting for pretreatment anticholinergicity, the authors found that mean serum anticholinergicity for the nortriptyline-treated patients was significantly greater than that for the paroxetine group at all weeks assessed. Serum anticholinergicity was significantly correlated with nortriptyline but not with paroxetine plasma levels. Complaints of dry mouth and tachycardia were significantly more frequent and severe in the nortriptyline group. CONCLUSIONS: These findings suggest that, at therapeutic plasma concentrations, paroxetine has approximately one-fifth the anticholinergic potential of nortriptyline in older patients.  相似文献   
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The rate of short-term memory search has previously been reported to be slower for older individuals than for college-age Ss (F. I. Craik, 1977). Current research has suggested that after extensive practice with the same population of stimuli, performance in memory-search and visual-search tasks can become "automatic," or independent of memory load. The present experiment examined age differences in the development of automatic processing in a hybrid memory-search/visual-search paradigm; 8 young (18–25 yrs old) and 8 older (61–74 yrs old) Ss participated. Although older Ss demonstrated a significantly slower rate of search, the 2 age groups shifted toward automatic processing, over practice, at equivalent rates. The slower rate of search thus represents an age-related increase in the time required to compare the memory-set items against those in a visual array, rather than a change in the mode of processing available. (20 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
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