Some Molecular and Cellular Stress Mechanisms Associated with Neurodegenerative Diseases and Atherosclerosis

Chronic stress is a combination of nonspecific adaptive reactions of the body to the influence of various adverse stress factors which disrupt its homeostasis, and it is also a corresponding state of the organism’s nervous system (or the body in general). We hypothesized that chronic stress may be one of the causes occurence of several molecular and cellular types of stress. We analyzed literary sources and considered most of these types of stress in our review article. We examined genes and mutations of nuclear and mitochondrial genomes and also molecular variants which lead to various types of stress. The end result of chronic stress can be metabolic disturbance in humans and animals, leading to accumulation of reactive oxygen species (ROS), oxidative stress, energy deficiency in cells (due to a decrease in ATP synthesis) and mitochondrial dysfunction. These changes can last for the lifetime and lead to severe pathologies, including neurodegenerative diseases and atherosclerosis. The analysis of literature allowed us to conclude that under the influence of chronic stress, metabolism in the human body can be disrupted, mutations of the mitochondrial and nuclear genome and dysfunction of cells and their compartments can occur. As a result of these processes, oxidative, genotoxic, and cellular stress can occur. Therefore, chronic stress can be one of the causes forthe occurrence and development of neurodegenerative diseases and atherosclerosis. In particular, chronic stress can play a large role in the occurrence and development of oxidative, genotoxic, and cellular types of stress.     

Advanced Method for Single Crystal Casting of Turbine Blades for Gas Turbine Engines and Plants

A method for fabricating single crystal blades that combines the techniques of seed crystals and selection is suggested. The method realizes the advantages of both techniques, i.e., the high structural perfection and the possibility of fabricating single crystals with specified spatial orientation. Metallographic and x-ray diffraction analyses are used to study the processes of nucleation of the single crystal structure of blade castings fabricated from high-temperature nickel alloys by the method of selection and seed crystals. A commercial process for fabricating cast single crystal turbine blades by the new method is suggested.     

Sequential discrete multilevel automated system for recording electron-diffraction patterns

This article considers a discrete sequential multilevel automated system for recording electron-diffraction patterns. A comparative analysis is presented, along with experimental results that make it possible to evaluate the effectiveness of the technical decisions on which the device is based. The general design of the system is presented. Translated from Izmeritel'naya Tekhnika No. 5, pp. 16–18, May, 1996.     

Synthesis of amphiphilic (meth)acrylates with oligo(ethylene glycol) and (or) oligo(propylene glycol) blocks by the esterification of (meth)acrylic acid

Thermoresponsive PEG-based (PEG stands polyethylene glycol) polymers are unique for use in medicine because of their low toxicity, good biocompatibility and biodegradability, but usually more hydrophobic and more toxic comonomers are used to adjust lower critical solution temperature (LCST). A convenient way to overcome this problem and to finely tune LCST is to use alkoxy oligo(ethylene glycol)- or alkoxy oligo(propylene glycol) (meth)acrylates as starting comonomers. Here we report on the conditions for the simple and affordable synthesis of methoxy oligo(propylene glycol) (meth)acrylate- and methoxy oligo(propylene glycol)-block-oligo(ethylene glycol) (meth)acrylate-based macromonomers with high yields (80%–98.7%) by the acid-catalyzed esterification of (meth)acrylic acid with alkoxy oligo(alkylene glycols) containing oligo(ethylene glycol) (OEG) and/or oligo(propylene glycol) (OPG) blocks. p-Toluene sulphonic acid (pTSA), alkyl(C₁₂–C₁₄)benzene sulfonic acid (ABSA) and H₂SO₄ were used as catalysts. It has been shown that pTSA and ABSA are practically the same in catalytic activity and are superior to sulfuric acid. The reaction orders with respect to catalyst was found to be close to 1 in all cases. It has been shown that the reaction is actually insensitive to the lengths of OEG and OPG blocks, as well as to the structure of the terminal alkyl group, while the esterification of acrylic acid (AA) proceeds much faster compared to methacrylic acid (MAA) one under the same conditions. The influence of temperature on the equilibrium conversions of alcohols was determined, which were found to be 89%–93% for the esterification of AA and 61%–86% for MAA in the temperature range of 60–120°C. A further increase in conversion was achieved by introducing an azeotropic agent (toluene), its optimal concentration was found to be 10%–15%.     

Hopping Conductivity in Poly(<Emphasis Type="Italic">p</Emphasis>-Xylylene)–Fe Nanocomposites

The structure and conductivity of hybrid nanocomposite poly(p-xylylene) films containing iron nanoparticles are studied. The films are obtained by the method of polymerization on a surface from the gas phase, and the concentration of the filler is 4 and 11 vol %. According to the analysis of microphotographs of nanostructures obtained by transmission electron microscopy, the particles are distributed evenly (with a size in the nanometer range and a rather narrow distribution). Studies of the frequency dependence of conductivity show that the transport of the charge carriers is realized by the hopping mechanism with a jump length of ~3.5 nm.     

Solution of sectorization problems for an air traffic management area. II. Development of sectorization algorithms

This is the continuation of the paper devoted to the study of airspace sectorization problems for air traffic areas as one of airspace design problems [1]. Algorithmic and technical aspects of solution of the problem of the first stage of airspace sectorization of a district center of air traffic management based on genetic optimization procedures are considered. Results of simulation of operation of optimization procedures are analyzed.     

Decladding of Irradiated Fuel Elements

The results of investigations of the leading operation of a nonaqueous technology for reprocessing fuel elements from nuclear reactors — dissolution of fuel claddings in a zinc-based melt — are presented. Data obtained in experiments on simulators and samples of irradiated fuel elements in standard BOR-60 and SM-2 packages with different burnup and holding time are presented. In the experiments, the metallic melt was separated from the fuel by filtering through a mesh and regenerated by vacuum distillation for reuse. The uranium and plutonium extraction was 99.99%. The behavior of individual radionuclides is described. __________ Translated from Atomnaya Energiya, Vol. 99, No. 4, pp. 273–276, October, 2005.     

Modified Low Density Lipoprotein and Lipoprotein-Containing Circulating Immune Complexes as Diagnostic and Prognostic Biomarkers of Atherosclerosis and Type 1 Diabetes Macrovascular Disease

In atherosclerosis; blood low-density lipoproteins (LDL) are subjected to multiple enzymatic and non-enzymatic modifications that increase their atherogenicity and induce immunogenicity. Modified LDL are capable of inducing vascular inflammation through activation of innate immunity; thus, contributing to the progression of atherogenesis. The immunogenicity of modified LDL results in induction of self-antibodies specific to a certain type of modified LDL. The antibodies react with modified LDL forming circulating immune complexes. Circulating immune complexes exhibit prominent immunomodulatory properties that influence atherosclerotic inflammation. Compared to freely circulating modified LDL; modified LDL associated with the immune complexes have a more robust atherogenic and proinflammatory potential. Various lipid components of the immune complexes may serve not only as diagnostic but also as essential predictive markers of cardiovascular events in atherosclerosis. Accumulating evidence indicates that LDL-containing immune complexes can also serve as biomarker for macrovascular disease in type 1 diabetes.     

Gaseous detector of ionizing radiation for registration of coherent neutrino scattering on nuclei

A method for registration of the coherent scattering reactor antineutrino on nuclei using a three-section low-background proportional counter was proposed. It is planned to use argon and xenon as the working substance. As has been shown on a test bench, pulse shape discrimination can effectively suppress the background from electromagnetic interference and microphonic effects in the energy range from 20 to 100 eV where the effect of coherent scattering of neutrinos on nuclei is expected with a factor of about 10³. Problems of the neutron background generated by cosmic-ray muons are analyzed. The scheme of the experimental setup is presented.