A Role for Human Renal Tubular Epithelial Cells in Direct Allo-Recognition by CD4+ T-Cells and the Effect of Ischemia-Reperfusion

Direct allorecognition is the earliest and most potent immune response against a kidney allograft. Currently, it is thought that passenger donor professional antigen-presenting cells (APCs) are responsible. Further, many studies support that graft ischemia-reperfusion injury increases the probability of acute rejection. We evaluated the possible role of primary human proximal renal tubular epithelial cells (RPTECs) in direct allorecognition by CD4+ T-cells and the effect of anoxia-reoxygenation. In cell culture, we detected that RPTECs express all the required molecules for CD4+ T-cell activation (HLA-DR, CD80, and ICAM-1). Anoxia-reoxygenation decreased HLA-DR and CD80 but increased ICAM-1. Following this, RPTECs were co-cultured with alloreactive CD4+ T-cells. In T-cells, zeta chain phosphorylation and c-Myc increased, indicating activation of T-cell receptor and co-stimulation signal transduction pathways, respectively. T-cell proliferation assessed with bromodeoxyuridine assay and with the marker Ki-67 increased. Previous culture of RPTECs under anoxia raised all the above parameters in T-cells. FOXP3 remained unaffected in all cases, signifying that proliferating T-cells were not differentiated towards a regulatory phenotype. Our results support that direct allorecognition may be mediated by RPTECs even in the absence of donor-derived professional APCs. Also, ischemia-reperfusion injury of the graft may enhance the above capacity of RPTECs, increasing the possibility of acute rejection.     

Viscous and Aeroelastic Effects on Wind Turbine Blades. The VISCEL project. Part I: 3D Navier–Stokes Rotor simulations

Aerodynamic modelling of HAWT rotors by means of “engineering methods” has reached a saddle point, where no further development can be expected without a breakthrough in understanding the physics of unsteady, rotating three‐dimensional flows. However, such a breakthrough becomes ever more necessary, as the size of the wind turbines increases. With the experimental work in that direction being mostly limited to observing the phenomena and interpreting the associated mechanisms, and its increased cost, alternatives are being sought. The use of CFD techniques and state‐of‐the‐art Navier–Stokes solvers is considered a very serious contender, a belief shared by the members of the present consortium, which has worked on the VISCEL JOR3‐CT98‐0208 Joule III project. This project's goal was to determine the aerodynamic characteristics as well as the aeroelastic behaviour of wind turbine blades across their broad range of operational conditions, from attached to highly separated flow regimes. The work programme included specific tasks for the validation and assessment of existing 3D solvers, for the parametric study of 3D flow around realistic blades and for the investigation of aeroelastic stability, at the blade section level. Copyright © 2003 John Wiley & Sons, Ltd.     

Microstructural changes accompanying repair welding in 5xxx aluminium alloys and their effect on the mechanical properties

At the shipyards, the aluminium alloy 5083 is welded with a multi-pass sequence using the metal inert gas technique. If, while checking the weld integrity either after welding or during service, defects are detected in the vicinity of the weldment, repairs are usually employed to extend the service life.

The repair method involves removal of the upper passes, depending on the thickness and re-welding under the same conditions.

Purpose of this paper is to examine the microstructural changes accompanying repair welding, define their effect on properties of primary importance and set, if possible, an upper limit as far as the number of repairs is concerned.     

Integrating mental health services within primary care. A Canadian program

The increasingly prominent role of the family physician in delivering mental health care can be enhanced if productive and collaborative relationships can be established with local mental health services. This paper describes a Canadian program that has achieved this by bringing mental health counselors and psychiatrists into the offices of 87 family physicians in 35 practices in a community in Southern Ontario. The paper describes the program, the activities of counselors and psychiatrists within the practices, and the administrative structures set up to coordinate these activities. Data is presented from the evaluation of the first year of the program's operation (13 practices and 45 family physicians) during which time 3085 referrals were received. The program made mental health care more available and accessible, increased continuity of care, provided additional support for the family physician, offered new opportunities for continuing education, and led to a reduced and more efficient use of other mental health services. The components of the program can be adapted to most communities.     

Segmentation of historical machine-printed documents using Adaptive Run Length Smoothing and skeleton segmentation paths

In this paper, we strive towards the development of efficient techniques in order to segment document pages resulting from the digitization of historical machine-printed sources. This kind of documents often suffer from low quality and local skew, several degradations due to the old printing matrix quality or ink diffusion, and exhibit complex and dense layout. To face these problems, we introduce the following innovative aspects: (i) use of a novel Adaptive Run Length Smoothing Algorithm (ARLSA) in order to face the problem of complex and dense document layout, (ii) detection of noisy areas and punctuation marks that are usual in historical machine-printed documents, (iii) detection of possible obstacles formed from background areas in order to separate neighboring text columns or text lines, and (iv) use of skeleton segmentation paths in order to isolate possible connected characters. Comparative experiments using several historical machine-printed documents prove the efficiency of the proposed technique.     

Text binarization in color documents

This article presents a new method for the binarization of color document images. Initially, the colors of the document image are reduced to a small number using a new color reduction technique. Specifically, this technique estimates the dominant colors and then assigns the original image colors to them in order that the background and text components to become uniform. Each dominant color defines a color plane in which the connected components (CCs) are extracted. Next, in each color plane a CC filtering procedure is applied which is followed by a grouping procedure. At the end of this stage, blocks of CCs are constructed which are next redefined by obtaining the direction of connection (DOC) property for each CC. Using the DOC property, the blocks of CCs are classified as text or nontext. The identified text blocks are binarized properly using suitable binarization techniques, considering the rest of the pixels as background. The final result is a binary image which contains always black characters in white background independently of the original colors of each text block. The proposed document binarization approach can also be used for binarization of noisy color (or gray‐scale) document images. Several experiments that confirm the effectiveness of the proposed technique are presented. © 2007 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 16, 262–274, 2006     

An integrated architecture for the provision of health telematic services based on digital library technologies

monolithic information system to effectively serve the needs of an entire healthcare organizational structure. Thus, information and telecommunications systems must primarily provide the infrastructure to support the effective integration of distributed and heterogeneous components, ensuring overall integrity in terms of functional and information interworking. This approach i.e., the integration of heterogeneous autonomous distributed systems, to developing and managing regional healthcare networks ensures the transfer and integration of consistent information between healthcare facilities, without imposing constraints on the operation of individual clinical units. This paper presents the results of an ongoing effort for the design and implementation of an architecture based on digital library technologies, for the provision of user-oriented telematic services in a regional healthcare network. Specifically, it addresses issues related to the provision of user-oriented services, transparent to the needs of different user groups and the requirements of specific tasks, based on: a) meta-information for the creation of an information infrastructure for the regional healthcare network which is, effectively, a multimedia distributed digital library, b) intelligent informationretrieval strategies to selectively retrieve information from multimedia data, c) agent-based technologies for effective service delivery adapted to the current user needs and the task at hand, and d) middleware services that explicitly reveal not only the characteristics of the information sources, but also address the context of specific telematic services, through appropriate mediation mechanisms. Received: 10 April 1997 / Revised: 6 July 1997 / Accepted: 5 August 1997     

Modeling heterogeneous bacterial populations exposed to antibiotics: The logistic‐dynamics case

In typical in vitro tests for clinical use or development of antibiotics, samples from a bacterial population are exposed to an antibiotic at various concentrations. The resulting data can then be used to build a mathematical model suitable for dosing regimen design or for further development. For bacterial populations that include resistant subpopulations—an issue that has reached alarming proportions—building such a model is challenging. In prior work, we developed a related modeling framework for such heterogeneous bacterial populations following linear dynamics when exposed to an antibiotic. We extend this framework to the case of logistic dynamics, common among strongly resistant bacterial strains. Explicit formulas are developed that can be easily used in parameter estimation and subsequent dosing regimen design under realistic pharmacokinetic conditions. A case study using experimental data from the effect of an antibiotic on a gram‐negative bacterial population exemplifies the usefulness of the proposed approach. © 2015 American Institute of Chemical Engineers AIChE J, 61: 2385–2393, 2015     

Control of a process with unmeasured disturbances that change its steady-state gain sign

Because the sign of the steady-state gain of an industrial NOx reduction unit changes according to the magnitude of the main external disturbance (hydrogen flow rate), designing an appropriate controller is particularly challenging. To address this issue, theoretical analysis suggested that a simple linear controller can provide adequate control for this process. Interestingly, tuning of this controller deviates from well known guidelines of linear control theory, which dictate that closed-loop stability is maintained by sluggish enough control of a stable linear process. It turns out, that for this nonlinear process, controller tuning must be neither too sluggish nor too aggressive, in an intermediate range suggested by a variant of the small-gain theorem proven here for corresponding nonlinear operators. The operator-based analysis was confirmed via computer simulation on a simple first-principles model, calibrated on real plant data. It is expected that control performance and robustness may improve if a number of ideas suggested in the text are explored further.