Collecting and processing records from the ultracentrifuge in "real-time" using an on-line computer

An analytical system consisting of an analytical cantrifuge coupled 'on-line' to a computer was assembled and tested. Collection of records from up to 9 solutions was achieved through programmes which sum readings to reduce noise as well as controlling the positioning of the scanner. With this system it was found that the limit on accuracy for molecular weights at concentrations less than 0.01 g cm-3 was +/- 3% estimated from sedimentation equilibrium experiments. The same system was used to collect records for similar concentrations from velocity experiments by employing a scanning schlieren. In this case the accuracy in estimating sedimentation coefficients was similar to those found when measuring photographs. Since the collection yields detailed information about the shape of the sedimenting boundary, the centroids of the boundary were routinely computed by second moment analysis rather than relying on the position of the maximum of the schlieren peak. In the same analysis estimates of diffusion coefficients were made routinely by calculating corrected height/area ratios for each scan. These calculations were made during the real-time of the experiment, so making available molecular parameters rather than records which must be evaluated some time after stopping the experiment.     

Talent from abroad: can it plug the gap? [overseas employment]

This paper discusses the problem of skills shortages in the UK and how overseas employment can be a good short-term solution. With skills in such short supply, the value of individual engineers increases significantly. Compounding this situation, massive investment in infrastructure and a corresponding demand for engineering talent has created an environment where employers fight for each candidate. While engineers from Eastern Europe and developing nations such as India can contribute in the short-term to reducing the skills gap, employers must be prepared for a slow start and significant expenses, especially as recruitment itself is not a significant industry and it takes a while to gain trust and understanding. For the long-term, it is important for the government to launch initiatives that would prioritize engineering as a career path.     

The effect of immediately preceding task brightness on visual performance.

To determine how visual performance at low photopic brightness levels is affected by the brightness of an immediately preceding visual task, Ss were required to read photographs of banks of instrument dials (a near-vision task) and banks of Landolt rings (a far-vision task) after adaptation to the brightness level of the first task, under speed and accuracy instructions. The Ss performed both the near and far tasks. Within the brightness ranges used, performance was related to the brightness of the task but not to the brightness of the immediately preceding task. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Residual temperature dependence of normalized diffusion of polystyrene latex in aqueous solvents

The so-called anomalous dependence of diffusion of polystyrene latex⁴ was examined in detail using quasi-elastic light scattering. The normalized diffusion coefficients showed a residual decrease with increasing temperature for solutions in water, 0.1 M phosphate buffers, methanol and 0.1 M sodium carbonate but not in dilute solutions of dimethyl ketone, Triton X-100, some salt solutions and pure ethanol. Using sensitive analyses for multi-exponential fits to the data, it was shown that the presence or absence of the dependence was not affected by possible low levels of aggregation of the latex. These results are interpreted as reflecting an increased solvation of the charge groups on the latex with rising temperature.     

Hydrogen production from bio-oil: A thermodynamic analysis of sorption-enhanced chemical looping steam reforming

The steam reforming of pyrolysis bio-oil is one proposed route to low carbon hydrogen production, which may be enhanced by combination with advanced steam reforming techniques. The advanced reforming of bio-oil is investigated via a thermodynamic analysis based on the minimisation of Gibbs Energy. Conventional steam reforming (C-SR) is assessed alongside sorption-enhanced steam reforming (SE-SR), chemical looping steam reforming (CLSR) and sorption-enhanced chemical looping steam reforming (SE-CLSR). The selected CO₂ sorbent is CaO(s) and oxygen transfer material (OTM) is Ni/NiO. PEFB bio-oil is modelled as a surrogate mixture and two common model compounds, acetic acid and furfural, are also considered. A process comparison highlights the advantages of sorption-enhancement and chemical looping, including improved purity and yield, and reductions in carbon deposition and process net energy balance.The operating regime of SE-CLSR is evaluated in order to assess the impact of S/C ratio, NiO/C ratio, CaO/C ratio and temperature. Autothermal operation can be achieved for S/C ratios between 1 and 3. In autothermal operation at 30 bar, S/C ratio of 2 gives a yield of 11.8 wt%, and hydrogen purity of 96.9 mol%. Alternatively, if autothermal operation is not a priority, the yield can be improved by reducing the quantity of OTM. The thermodynamic analysis highlights the role of advanced reforming techniques in enhancing the potential of bio-oil as a source of hydrogen.     

Performance and damage evolution of plain and fibre-reinforced segmental concrete pipelines subjected to transverse permanent ground displacement

This paper presents the results of three full-scale experiments performed on segmental concrete pipelines subjected to permanent ground displacement. The first pipeline was made of reinforced concrete pipes and the second pipeline was made of steel fibre-reinforced concrete pipes. The third pipeline was made of a combination of fibre-reinforced and reinforced concrete pipes. An array of sensing techniques was used to assess the damage evolution in pipelines and their overall performance. Three stages of damage were observed. In the first stage, damage was concentrated in the joints near the fault line. In the second stage, the damage occurred in all joints along the pipeline. While in the first two stages damage was mainly concentrated at the bell and spigot joints of the pipe segments, the third stage of damage was characterised by severe damage and rupture of the body of pipe segments located in the immediate vicinity of the fault line. The modes of failure for the plain and fibre-reinforced concrete pipelines were similar in the first and second stages of damage. However, in the pipeline constructed using both plain and fibre-reinforced concrete pipe segments, the damage was concentrated in the standard reinforced concrete pipe segments.     

Human SP-A locus: allele frequencies and linkage disequilibrium between the two surfactant protein A genes

Two surfactant protein A (SP-A) genes and several alleles for each SP-A locus have been previously described. In this report we investigate the potential usefulness of the SP-A loci as markers for genetic studies. We establish conditions that allow the identification of alleles with very similar sequences; We also determine the degree of polymorphism for each SP-A locus: The heterozygosity and polymorphism information content (PIC) values for the SP-A1 locus are 0.63 and 0.55, respectively, and for the SP-A2 locus are 0.50 and 0.56. In the course of these studies, we identify one new SP-A2 allele and show that the SP-A1 and SP-A2 loci are in linkage disequilibrium (P < 0.000001). We also identify 19 of the 20 possible haplotypes in a population of n = 239. Nine of the observed haplotypes reach statistical significance (P < 0.01) in this population, and the segregation of two haplotypes (6A2/1A0 and 6A4/1A) without recombination is verified in a family pedigree. These data together indicate that both SP-A loci are sufficiently polymorphic to be good markers for use in genetic studies. Furthermore, the finding of one novel allele suggests that additional unknown SP-A alleles are yet to be found.     

Immunotargeting of liposomes to activated vascular endothelial cells: a strategy for site-selective delivery in the cardiovascular system

Endothelial-selective delivery of therapeutic agents, such as drugs or genes, would provide a useful tool for modifying vascular function in various disease states. A potential molecular target for such delivery is E-selectin, an endothelial-specific cell surface molecule expressed at sites of activation in vivo and inducible in cultured human umbilical vein endothelial cells (HUVEC) by treatment with cytokines such as recombinant human interleukin 1beta (IL-1beta). Liposomes of various types (classical, sterically stabilized, cationic, pH-sensitive), each conjugated with mAb H18/7, a murine monoclonal antibody that recognizes the extracellular domain of E-selectin, bound selectively and specifically to IL-1beta-activated HUVEC at levels up to 275-fold higher than to unactivated HUVEC. E-selectin-targeted immunoliposomes appeared in acidic, perinuclear vesicles 2-4 hr after binding to the cell surface, consistent with internalization via the endosome/lysosome pathway. Activated HUVEC incubated with E-selectin-targeted immunoliposomes, loaded with the cytotoxic agent doxorubicin, exhibited significantly decreased cell survival, whereas unactivated HUVEC were unaffected by such treatment. These results demonstrate the feasibility of exploiting cell surface activation markers for the endothelial-selective delivery of biologically active agents via immunoliposomes. Application of this targeting approach in vivo may lead to novel therapeutic strategies in the treatment of cardiovascular disease.     

Sustained submaximal exercise does not alter the integrity of the lung blood-gas barrier in elite athletes

We have constructed model membrane proteins with hydrophobic segments of the general composition Leu29Val-Leun where n=10 and 20, and have analyzed their transmembrane topology when inserted into microsomal membranes. These hydrophobic segments span the membrane once, even though they are twice as long as normal transmembrane helices. Strikingly, a single proline residue introduced near the center of the Leu39Val hydrophobic stretch induces the formation of two transmembrane segments separated by a tight turn. These results have implications for our understanding of membrane protein assembly in the endoplasmic reticulum, and for the development of techniques for predicting membrane protein topology.