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1.
Activation and Thr286 autophosphorylation of calcium/calmodulindependent kinase II (CaMKII) following Ca2+ influx via N-methyl-D-aspartate (NMDA)-type glutamate receptors is essential for hippocampal long term potentiation (LTP), a widely investigated cellular model of learning and memory. Here, we show that NR2B, but not NR2A or NR1, subunits of NMDA receptors are responsible for autophosphorylation-dependent targeting of CaMKII. CaMKII and NMDA receptors colocalize in neuronal dendritic spines, and a CaMKII.NMDA receptor complex can be isolated from brain extracts. Autophosphorylation induces direct high-affinity binding of CaMKII to a 50 amino acid domain in the NR2B cytoplasmic tail; little or no binding is observed to NR2A and NR1 cytoplasmic tails. Specific colocalization of CaMKII with NR2B-containing NMDA receptors in transfected cells depends on receptor activation, Ca2+ influx, and Thr286 autophosphorylation. Translocation of CaMKII because of interaction with the NMDA receptor Ca2+ channel may potentiate kinase activity and provide exquisite spatial and temporal control of postsynaptic substrate phosphorylation. 相似文献
2.
Peele AG Nugent KA Rode AV Gabel K Richardson MC Strack R Siegmund W 《Applied optics》1996,35(22):4420-4425
We report an experimental investigation and comparison with simulation of the x-ray focusing of a flat, square profile microchannel plate. We use x rays with an energy of ~1.5 keV from a laser-produced plasma. The images were recorded with x-ray film. We find the focal structure to be consistent with theoretical expectations. The angular resolution of the focus is 0.96 mrad, which is a major improvement over previous results. The measured peak intensity gain is 27 ± 4, which is ~33% of that for a perfect optic. 相似文献
3.
The authors apply an embodied account to mere exposure, arguing that through the repeated exposure of a particular stimulus, motor responses specifically associated to that stimulus are repeatedly simulated, thus trained, and become increasingly fluent. This increased fluency drives preferences for repeated stimuli. This hypothesis was tested by blocking stimulus-specific motor simulations during repeated exposure. In Experiment 1, chewing gum while evaluating stimuli destroyed mere exposure effects (MEEs) for words but not for visual characters. However, concurrently kneading a ball left both MEEs unaffected. In Experiment 2, concurrently whispering an unrelated word destroyed MEEs for words but not for characters, even when implemented either exclusively during the initial presentation or during the test phase and when the first presentation involved an evaluation or a mere study of the stimuli. In Experiment 3, a double dissociation between 2 classes of stimuli was demonstrated, namely, words (oral) and tunes (vocal). A concurrent oral task (tongue movements) destroyed MEEs for words but not for tone sequences. A concurrent vocal task (humming “mm-hm”) destroyed MEEs for tone sequences but not for words. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Investigated the social responses to dysphoria of 120 female undergraduates selected on the basis of their scores on the Beck Depression Inventory. Ss conversed for 15 min with persons selected for presence or absence of depressed mood. Following the conversations, mood measures (e.g., Multiple Affect Adjective Check List) were administered along with social perception questionnaires that were described either as confidential or to be shared with the other person. Ss who interacted with depressed persons were anxious, depressed, and hostile, and Ss rejected them. Contrary to predictions, Ss were willing to share their negative responses with the depressed persons. The depressed persons correctly anticipated rejection and reciprocated. It is argued that cognitive models of depression need to be integrated with a conception of the social environment as being active and responsive. Judgments of cognitive distortion cannot be made without an understanding of the feedback typically available from the social environment. (34 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
7.
Quality improvement may be defined as the effort to improve the level of performance of key processes in the ICU. Based on knowledge of the current level of performance and on systematic analysis of organizational aspects of the ICU, quality improvement will include the implementation of standards, the formulation and analysis of quality indicators (e.g. scores, rate of complications such as nosocomial infections) and the possible introduction of data management systems. 相似文献
8.
Strack Fritz; Schwarz Norbert; Gschneidinger Elisabeth 《Canadian Metallurgical Quarterly》1985,49(6):1460
Conducted 3 experiments to determine the affect of reminiscing on reported well-being. 51 students at a professional school for translators and interpreters in Exp I and 36 undergraduates in Exp II recounted events that they had experienced as positive and pleasant or as negative and unpleasant. In Exp III, 64 undergraduates wrote down a particularly positive or negative event and then asked to explain either why or how this event occurred. Ss in all 3 experiments were then asked to rate their happiness and life satisfaction. Overall results indicate that Ss' ratings of general life satisfaction depended not only on the hedonic quality of the life experiences they happened to recall but also on the way in which they thought about them. Specifically, the hedonic quality of present life events influenced Ss' judgments of well-being in the same direction. The hedonic quality of past events, however, had a congruent impact on well-being judgments only when thinking about them elicited affect in the present but otherwise had a contrast effect on these judgments. Two factors were found to determine if thinking about the past elicits affect: whether Ss describe the events vividly and in detail or only mention them briefly, and whether Ss describe how the events occurred rather than why they occurred. (22 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
9.
Strack G Pita M Ornatska M Katz E 《Chembiochem : a European journal of chemical biology》2008,9(8):1260-1266
Biochemical systems that demonstrate the Boolean logic operations AND, OR, XOR, and InhibA were developed by using soluble compounds, which represent the chemical "devices", and the enzymes glucose oxidase (GOx), glucose dehydrogenase (GDH), alcohol dehydrogenase (AlcDH), and microperoxidase-11 (MP-11), which operated as the input signals that activated the logic gates. The enzymes were used as soluble materials and as immobilized biocatalysts. The studied systems are proposed to be a step towards the construction of "smart" signal-responsive materials with built-in Boolean logic. 相似文献
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