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1.
Dr. Yibin Zhang Dr. Shuai Xia Shulin Wan Tessa E. Steenwinkel Tara Vohs Prof. Rudy L. Luck Prof. Thomas Werner Prof. Haiying Liu 《Chembiochem : a European journal of chemical biology》2021,22(13):2282-2291
Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae. 相似文献
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Mari Beth Coleman Kristen Fowler Alexandra Lee Parater Marie Jackson Riley Yujeong Park Tara C. Moore 《Journal of Computer Assisted Learning》2021,37(3):759-772
Technology-assisted instruction has potential for helping students improve their reading skills. In the current studies, PowerPoint software was used to supplement teacher-led reading instruction for elementary-aged students with disabilities who struggled with phoneme blending to read words. The effectiveness of the intervention was assessed using two multiple probe design studies across sets of words based on an onset-rime (word family) strategy. In Study 1, teacher-led instruction was paired with technology-assisted instruction for all intervention sessions for three students in first and second grade. In Study 2, teacher-led instruction occurred between baseline and technology-assisted intervention sessions for three students in third and fourth grade. During the initial instruction sessions, participants recorded their own voices onto PowerPoint slides so that their technology-assisted instruction included self-modelling. Results of both studies indicated that technology-assisted instruction can be effective for helping students learn how to blend phonemes to read words; however, performance varied and there were limitations in each study that should be addressed in future research. Relevant implications for classroom instruction are emphasized. 相似文献
4.
E. I. Basaldella J.C. Tara G. Aguilar Armenta M. E. Patiño Iglesias 《Journal of Porous Materials》2007,14(3):273-278
Samples of mesoporous silica SBA-15 were prepared under hydrothermal conditions where Cu cations were incorporated to the
structure by impregnation in order to compare the adsorption behavior in the presence and absence of this element. The adsorption/desorption
equilibrium isotherms of propylene, propane, and N2 were measured to evaluate their usefulness in the propane/propylene separation.
All the adsorption isotherms of SBA-15 measured in the absence of Cu cations were described by the Freundlich equation, while
the adsorption isotherms of propane on Cu/SBA-15 were better represented by the Henry equation and those of propylene were
satisfactorily described by the Langmuir model in the range P < 100 Torr. The adsorption uptake of propylene increased and that of propane decreased in Cu/SBA-15 as compared to the amounts
observed in the SBA-15 sample. The presence of Cu atoms in the adsorbent lattice increased the selectivity towards propylene.
Under some working conditions the adsorbed amount of propylene in Cu/SBA-15 sample was totally reversible and the propane
uptake, negligible. 相似文献
5.
Avi Parush Chelsea Kramer Tara Foster-Hunt Alicia McMullan Kathryn Momtahan 《Cognition, Technology & Work》2014,16(1):47-57
Teamwork is prevalent in many work contexts. This study explored the similarities and differences in teamwork processes across different healthcare work contexts with the aim of assessing knowledge transfer feasibility. The research approach was to aggregate team communication analyses from four healthcare contexts to uncover teamwork similarities and differences. The four healthcare contexts included two handoffs and two surgery contexts. The communication analysis segmented communication into meaningful sequences. It categorized utterances into content categories and verbal behaviors. There were a few similar content categories across the four contexts. A clear information structure emerged in the two handoff contexts. In addition, there were more dialogues and requests in the surgeries compared to more reports in the handoffs. The content similarities suggest that some knowledge is transferable among the contexts. However, the differences in communication patterns reflect fundamental differences between handoff and surgery contexts in some teamwork processes. This research demonstrated that using communication analysis can uncover similarities and differences in team cognition and teamwork processes across work contexts. This in turn can help determine what knowledge and methods pertaining to team training, procedures, and technology are transferable across the contexts. 相似文献
6.
Paul J Tikalsky Della RoyBarry Scheetz Tara Krize 《Cement and Concrete Research》2002,32(8):1239-1246
Experimental research was performed to relate specific cement characteristics to expansion due to sulfate attack. Twenty-one North American cement of statistically diverse chemical composition were used in the study. ASTM 1012 “Standard Test Method for Length Change of Hydraulic Cement Mortars Exposed to a Sulfate Solution” was performed using mortars prepared with each of the cement. First-order and multivariate relationships between cement characteristics and sulfate expansion were correlated at different ages. Analysis revealed that while tricalcium aluminate (C3A) has typically been targeted as the chief contributor to sulfate attack, iron oxide (Fe2O3) or tetracalcium aluminoferrite (C4AF) content, combined with total equivalent alkalis, showed a much stronger negative correlation with expansions at all ages. These results are in agreement with a broad spectrum of sulfate expansion theories and can provide a better means of specifying sulfate-resistant cement. 相似文献
7.
The US workforce is aging. At the same time, there are a record number of open positions in the transportation sector, which has traditionally been a well-paying, but stressful and schedule-dependent, occupation. Due to increasing longevity, need, and ability to work, a possible solution to the transportation workforce shortfall may lie within the retention and recruitment of older workers. This paper uses a socio-technical framework to examine the pertinent, though scant, literature and data related to older workforce demographics and operational needs, the regulatory environment, requisite knowledge, skills and abilities, and application of support technology and training. Although there is evidence of age-related changes in physiology and cognition, the current science remains unable to resolve how an older workforce may most appropriately be applied to transportation to maximize system safety and minimize negative impact to worker well-being. 相似文献
8.
This work‐in‐process literature review gives an overview of recent insight in the incorporation of social media in risk and crisis communication. By marrying literature and examples of social media use with best practices in risk and crisis communication, this study demonstrates how communicators can embrace social media tools to better manage a risk or crisis. Best practices in risk and crisis communication are summarized, examples of social media tools used to manage risks and crises are expounded, and recommendations for practitioners are provided to incorporate social media tools in risk and crisis communication. 相似文献
9.
The body of a walking human is an elaborated dynamic system that operates adaptively in various conditions such as fast walking. Due to dynamic redundancies, the individual motor control strategies for speeding up the walking can be different among normal subjects. However, in reality, we see that the pattern of motion is quite similar among people and it is only the profile of hip joint motion along its path which determines the speed. The objective of the current paper is to develop a mathematical framework to investigate time optimal motion of a human during walking. To this end, a nine-link planar biped model is used. The motion is considered to take place in sagittal plane and to follow a normal pattern of motion. The solution is obtained using a phase plane method to solve minimum time problem which is subjected to inequality constraints of variable maximum joint torques and stability conditions. The solution method can be used to find the maximum possible speed of a human with specific body characteristics and to obtain a hip joint trajectory which could produce that speed. The proposed method can be utilized to study quantitative effect of different parameters such as joint strength in fast walking. 相似文献
10.
The temperature dependence of the carrier scattering rate in copper, silver, and gold at low temperatures has been determined from magnetoacoustic data. Both the (100) central belly orbit and the (110) dogs-bone orbit exhibit a T
3 dependence in all three metals. For copper and silver the coefficients of the corresponding term in the scattering rate agree well with those obtained from radiofrequency size-effect experiments. Previously reported discrepancies between the results for copper obtained by the two techniques are discussed and tentatively resolved. No effects due to electron-electron scattering are observed. Differences in the scattering rates between the noble metals appear to be primarily correlated with changes in the phonon density of states.Work supported by National Science Foundation. 相似文献