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1.
In this report, we present evidence that the CTL response directed against MHC Class I allo-determinants can be inhibited as a result of IL-10 expression in vivo. The presence of localized IL-10 secretion at the site of allogeneic tumor cell challenge resulted in marked inhibition of the CTL response and allowed growth of the tumor in the allogeneic host. Using purified CD4+ T cells from mice immunized in the presence or absence of IL-10, we have shown that the loss of alloreactivity as a consequence of IL-10 expression results from the inhibition of CD4+ T cell function. The expression of either IL-2 or IFN-gamma with IL-10 locally at the time of allogeneic cell challenge completely restored CTL alloreactivity, suggesting that the action of IL-10 could be bypassed by providing helper T lymphocyte-derived cytokines of the Th1 type at the site of immunization. Inhibition of alloreactivity by IL-10 was observed using either purified macrophages or dendritic cells as APC in an in vitro assay. Thus, the expression of IL-10 following antigenic challenge (such as that observed in Th2-like immune responses) may profoundly limit the ability for generating functional CTL in vivo. 相似文献
2.
Samaha MA Ochanda FO Tafreshi HV Tepper GC Gad-el-Hak M 《The Review of scientific instruments》2011,82(4):045109
Light scattering was used to measure the time-dependent loss of air entrapped within a submerged microporous hydrophobic surface subjected to different environmental conditions. The loss of trapped air resulted in a measurable decrease in surface reflectivity and the kinetics of the process was determined in real time and compared to surface properties, such as porosity and morphology. The light-scattering results were compared with measurements of skin-friction drag, static contact angle, and contact-angle hysteresis. The in situ, noninvasive optical technique was shown to correlate well with the more conventional methods for quantifying surface hydrophobicity, such as flow slip and contact angle. 相似文献
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Tepper Bennett J.; Duffy Michelle K.; Hoobler Jenny; Ensley Michael D. 《Canadian Metallurgical Quarterly》2004,89(3):455
The authors developed and tested the prediction that the relationship hetween coworkers' organizational citizenship behaviors (OCBs) and fellow employees' attitudes depends on the supervisors' abusiveness. Results of a longitudinal study using data collected from 173 supervised employees at 2 points in time (separated by 7 months) suggested that coworkers' OCB was positively related to fellow employees' job satisfaction and affective commitment when abusive supervision was low. However, when abusive supervision was high, coworkers' OCB was negatively related to job satisfaction and was unrelated to organizational commitment. The results of a 2nd study were consistent with the idea that the attributions employees make for their coworkers' OCB explains the moderating effect of abusive supervision on the relationship between coworkers' OCB and job satisfaction. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
5.
Lipid metabolism of male Fisher 344 rats aged 2–24 months was studied. Serum and liver cholesterol levels did not display
the age-related gradual increase seen in other rat strains. An increase in the serum plus liver cholesterol pool from 2 to
6 months was followed by a plateau through 18 months and then another increase at 24 months of age. The triglyceride pool
increased from 2 to 6 months and then remained unchanged through 24 months of age. Cholesterol synthesis from acetate decreased
50% between 2 and 9 months and fell only slightly through 24 months of age. Assay of 3-hydroxy-3-methyl glutaryl Coenzyme
A (HMG-CoA) reductase showed a similar pattern but did not decrease further after 9 months of age. Cholesterol 7α hydroxylase
activity was not significantly altered by age. These age- and strain-related differences present an opportunity for a comparative
study of the aging process using the parameters of lipid metabolism as indicators. 相似文献
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Anestos Themos C. Sickles James E. Tepper Richard M. 《Industry Applications, IEEE Transactions on》1977,(2):168-177
The dependence of the charge to mass ratio or specific charge of the spray stream on fluid and automization parameters in electrostatic spraying of various solvents and paints has been investigated. In addition, atomized paint particle sizes have been analyzed. It has been found that an optimum value of fluid conductivity exists at 2 ?ho/cm where the specific charge of the spray stream exhibits a maximum. From the particle size distribution and from the dependence of the specific charge of the spray stream on conductivity it has been concluded that the basic spray droplet charging mechanism is due to the breakup of a charged liquid surface, while corona discharge does not appear to play a significant role in the charging of the particles. 相似文献
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The use of AC potentials in electrospraying and electrospinning processes was demonstrated. Carboxymethylcellulose (CMC) was electrosprayed onto semiconducting and insulating substrates using both DC and AC potentials. On the semiconducting substrate, both AC and DC methods were capable of producing significant CMC coverage. However, only the AC potential was capable of producing significant coverage on the insulating substrate, possibly due to a reduction in the amount of surface charging. In the electrospinning investigation, poly(ethylene oxide) (PEO) fibers were spun into mats using both DC and AC driving potentials. The AC potential resulted in a significant reduction in the amount of fiber ‘whipping’ and the resulting mats exhibited a higher degree of fiber alignment but were observed to contain more residual solvent. The average fiber diameter for both DC and AC-spun mats exhibited a strong dependence on solution concentration. 相似文献
10.
Solvent-free, photoreactive particles of 2,5-distyrylpyrazine (DSP) monomer were developed by rapid precipitation from an expanding supercritical chlorodifluoromethane solution. DSP polymer particles were produced by solid-state photopolymerization. DSP particles below a critical diameter of about 0.5 μm were found to be mechanically stable and did not fragment upon photopolymerization. The rate of DSP photopolymerization was shown to be size-sensitive. Nano-scale particles demonstrated superior photoreactivity in the solid state in comparison to micro-scale crystals. UV spectra of DSP at different degrees of conversion were investigated and the extinction coefficients were calculated for the DSP monomer and polymer in sulfuric acid. 相似文献