Identification of atypical Aeromonas salmonicida: inter-laboratory evaluation and harmonization of methods

Estrogen therapy increases plasma HDL levels, which may reduce cardiovascular risk in postmenopausal women. The mechanism of action of estrogen in influencing various steps in hepatic HDL and apolipoprotein (apo) A-I synthesis and secretion are not fully understood. In this study, we have used the human hepatoblastoma cell line (Hep G2) as an in vitro model system to delineate the effect of estradiol on multiple regulatory steps involved in hepatic HDL metabolism. Incubation of Hep G2 cells with estradiol resulted in the following statistically significant findings: (1) increased accumulation of apoA-I in the medium without affecting uptake/removal of radiolabeled HDL-protein; (2) accelerated incorporation of [3H]leucine into apoA-I; (3) selective increase in [3H]leucine incorporation into lipoprotein (LP) A-I but not LP A-I+A-II HDL particles (HDL particles without and with apoA-II, respectively); (4) increased ability of apoA-I-containing particles to efflux cholesterol from fibroblasts; (5) stimulated steady state apoA-I but not apoA-II mRNA expression; and (6) increased newly transcribed apoA-I mRNA message without effect on apoA-I mRNA half-life. The data indicate that estradiol stimulates newly transcribed hepatic apoA-I mRNA, resulting in a selective increase in LP A-I, a subfraction of HDL that is associated with decreased atherosclerotic cardiovascular disease, especially in premenopausal women.     

Dynamic and CW linewidth measurements of 1.55-μm InGaAs-InGaAsPmultiquantum well distributed feedback lasers

Measurement of the CW linewidth and frequency chirp as functions of modulation data rate and bias level for 1.55-μm InGaAsP multiquantum-well distributed feedback lasers grown by low-pressure MOCVD are presented. The results show that the CW linewidth of asymmetric facet-coated multiquantum-well DFB lasers can be as low as 2.0 MHz at 13.5 mW output power. The frequency chirp increases with modulation data rate and is significantly larger if the laser off-state is below threshold than if it is above threshold. The 20 dB down chirp widths are in the range of 1.9-5 Å for 40 mA peak-to-peak modulation current at 10 Gb/s under above-threshold bias     

Diagnosis of idiopathic myositis: value of 99mtechnetium pyrophosphate muscle scintigraphy and magnetic resonance imaging in targeted muscle biopsy

The widespread use and dramatic success of prosthetic joint implant surgery (arthroplasty) has greatly improved the quality of life for many individuals suffering from degenerative, arthritic, or injured joints. However, in a number of cases, recipients experience discomfort at the arthroplasty site that can signal biomechanical failure, or periprosthetic infection of the joint, or both. In fact, infection remains a devastating obstacle, preventing arthroplasty from being an almost perfect medical procedure. Existing tests for the diagnosis of infection in orthopedics are disappointing because of the relatively high frequency of false negative results. To overcome this shortcoming, and to reduce the number of infected revision arthroplasties, a methodology based on molecular biology has been established. The method allows the rapid, sensitive and accurate diagnosis of orthopedic infections, and should aid the clinician with treatment regimens, surgical decisions, and overall patient management in the growing arthroplasty population.     

Carrier loss in InGaAsP-InP lasers grown by hydride CVD

We have experimentally characterized the quantum efficiency in InGaAsP hydride CVD grown lasers operating at 1.3 μm. The observed reduction in external quantum efficiency with increasing temperature is found to be caused mostly by a reduction of the internal quantum efficiency. The experimental results are well explained with a theoretical model based on thermionic emission of carriers out of the active region. The model also helps to understand the difference in temperature performance between lasers studied in this paper and those grown by MOCVD