Purification of the cardiac sarcoplasmic reticulum membrane protein phospholamban from recombinant Escherichia coli

Phospholamban (PLN) was expressed in Escherichia coli as a protein fusion with glutathione S-transferase (GST). GST-PLN was mostly present in the insoluble protein fraction and accounted for approximately 50% of total insoluble protein. Attempts to suppress inclusion body formation or to use GST as an affinity-purification tag failed. A successful purification method is based on preparative SDS/PAGE and electrodialysis. From 1 g cells we typically purified 13.5 mg fusion protein with a PLN content of 2.8 mg. We genetically inserted an enterokinase (EK) protease site just in front of the PLN sequence and demonstrated the proteolytical liberation of PLN from the carrier protein. The approach described represents a substantial advancement in PLN expression and purification.     

A delta transform approach to loop gain-phase shaping design of robust digital control systems

This paper addresses the existence of loop gain-phase shaping (LGPS) solutions for the design of robust digital control systems for SISO, minimum-phase, continuous-time processes with parametric uncertainty. We develop the frequency response properties of LGPS for discrete-time systems using the Δ-transform, a transform method that applies to both continuous-time and discrete-time systems. A theorem is presented which demonstrates that for reasonable specifications there always exists a sampling period such that the robust digital control problem has a solution. Finally, we offer a procedure for estimating the maximum feasible sampling period for LGPS solutions to robust digital control problems.     

Die zweite Phase der Feldbusentwicklung

The past decade may be regarded as the phase of the fundamental developments in fieldbus systems that were based on the OSI model. Today we are entering a second stage. It is the objective of this issue to present the current state and where the further developments will lead to. This introductory article is intended to give an overview on the subject.     

Auswirkungen von Blitzschlägen auf 20-kV-Energiekabel

Recently appeared defects on high voltage cables have required an urgent evaluation of the impact of lightning strikes necessary. In some cases overvoltages between the copper wire screen of the cable and earth may appear which cause damages of the cablesheath. Field measurements with a classification of the voltage impulses at the copper wire screen have been made to evaluate the number of events. Additionally a numerical model was developed to study the effects of atmospheric discharges on underground cables with emphasis to the influence on the cablesheath. This numeric method was also applied to estimate the effectiveness of different protection arrangements. This contribution presents evaluation methods for overvoltages of cables and a model used firstly for calculating the stress caused by differential voltages and secondly to determinate the influence of protection measures.     

Measurements of frequencies and spatial correlations of coherent structures in rod bundle flows

Cross-wire anemometry was used to identify and characterize coherent flow pulsations in isothermal air flow near the gap regions of a five-rod bundle with a design pitch-to-diameter ratio of 1.149 and contained in a quasi-trapezoidal duct. It was confirmed that such pulsations are quasi-periodic and contribute significantly to the velocity fluctuations across the gap. The frequency of pulsations was found to decrease with diminishing rod–wall gap size in the range between 0.015D and 0.250D, where D is the rod diameter. The pulsations in a rod–wall gap and an adjacent rod–rod gap were strongly coupled and occurred at the same frequency as one rod was displaced towards the duct wall.     

38. Generalversammlung von CENELEC

Ohne Zusammenfassung     

Qualitativ-quantitative Simulation, ein Instrument zur Analyse nichtlinearer Regelkreise

Qualitative-quantitative simulation is one of the most productive and promising research areas in Artificial Intelligence to emerge in recent years. This method provides an elegant way for an exhaustive analysis of uncertain dynamic systems. The application of the qualitative-quantitative simulation is shown in this paper. The important question about the stability of a quanlitatively described dynamical system is solved by a novel analytic method.