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1.
The interaction of cholesterol with ceramides containing α-hydroxy fatty acyl chains (hydroxyceramides) has been studies as
a foundation for characterizing the lipid bilayers of thestratum corneum. A relatively large quantity of cerebrosides was obtained from bovine brain and converted to ceramides through removal of
the carbohydrate side chain. The ceramides were separated based on the absence or presence of hydroxy fatty acyl chains. The
lyophilized hydroxyceramides showed a broad melting region at 92°C. Hydroxyceramides dispersed in water produced a relatively
narrow, thermotropic transition at 75°C. The effect of cholesterol on this thermotropic phase transition of hydroxyceramides
was determined by differential scanning calorimetry. With respect to the main transition, cholesterol caused a broadening
of the phase transition at relatively low levels as well as a decrease in the peak transition temperature. The presence of
cholesterol at levels in excess of 7 wt% gave rise to an additional low-temperature transition at 55°C. Upon immediate rescanning,
this transition was exothermic, but with increasing incubation time the area under the excess heat capacity curve as a function
of temperature became smaller. After two days or more, the transition observed was endothermic. At cholesterol levels between
40 and 50 wt%, multiple peaks were observed. From comparisons with related systems, the cooperative thermal transitions of
hydroxyceramides with cholesterol are suggested to result from changes in hydrogen bonding or be due to phase separation.
The composition of isolated brain ceramides is being compared with that reported for thestratum corneum. 相似文献
2.
Economic and process engineering advantages of extrusion cooking in comparison with conventional processes in the food industry . Extrusion cooking is introduced as a modern high-temperature short-time process. Possible process steps in extruder machine systems, such as continuous conveying, mixing, homogenizing and reactions, mechanical and thermal energy dissipation for plasticizing and modifying biopolymers, are explained. This process creates new products with completely new textures, for example in the snack and breakfast cereal sector, while on the other hand the extrusion process competes with long-standing conventional processes. The latter work slowly with low pressures, low temperatures and energy dissipation with high water contents in large batch-operated machines. The quality of extruder-cooked products depends on the extrusion system. The self-cleaning corotating twin-screw extruder with its narrow residence time spectrum is the optimum system. However, the counter-rotating intermeshing machine has certain advantages for products of low viscosity. The cooking time at high temperature is a matter of seconds, which serves to maintain the properties of the ingredients and active substances, while ensuring fast destruction of microorganisms. The end-products have a long shelf-life on account of their low process moisture content. Continuous extrusion cooking has economic advantages mainly because it replaces many batch processes and because extrusion is carried out entirely or almost entirely with the final moisture content, thus avoiding the necessity to evaporate huge quantities of water. 相似文献
3.
Listeriosis is a foodborne disease caused by the bacterium Listeria monocytogenes. The food industry and government agencies devote considerable resources to reducing contamination of ready-to-eat foods with L. monocytogenes. Because inactivation treatments can effectively eliminate L. monocytogenes present on raw materials, postprocessing cross-contamination from the processing plant environment appears to be responsible for most L. monocytogenes food contamination events. An improved understanding of cross-contamination pathways is critical to preventing L. monocytogenes contamination. Therefore, a plant-specific mathematical model of L. monocytogenes cross-contamination was developed, which described the transmission of L. monocytogenes contamination among food, food contact surfaces, employees' gloves, and the environment. A smoked fish processing plant was used as a model system. The model estimated that 10.7% (5th and 95th percentile, 0.05% and 22.3%, respectively) of food products in a lot are likely to be contaminated with L. monocytogenes. Sensitivity analysis identified the most significant input parameters as the frequency with which employees' gloves contact food and food contact surfaces, and the frequency of changing gloves. Scenario analysis indicated that the greatest reduction of the within-lot prevalence of contaminated food products can be achieved if the raw material entering the plant is free of contamination. Zero contamination of food products in a lot was possible but rare. This model could be used in a risk assessment to quantify the potential public health benefits of in-plant control strategies to reduce cross-contamination. 相似文献
4.
Josef Anselm Wiedmann 《化学,工程师,技术》1983,55(9):689-700
The flooding behaviour of two- and three-phase operating stirred reactors . The present state of research concerning the flooding behaviour of aerated fluids and suspensions is presented. The author's own investigations, undertaken partly on a semi-technical scale (tank diameter D = 1.5 m) show the most important parameters of this undesirable aerated state during the operation of stirred reactors. For a given stirrer speed the superficial gas velocity, the tank size, and the fluid viscosity are important. Solid particles sediment out when flooding is reached. An objective measuring method for determining the occurrence of flooding was adopted. 相似文献
5.
Fr. Wiedmann 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1904,8(2):136-139
Ohne ZusammenfassungMitteilung aus dem chemischen Untersuchungsamte der Stadt Chemnitz. 相似文献
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LW Wattenberg TS Wiedmann RD Estensen CL Zimmerman VE Steele GJ Kelloff 《Canadian Metallurgical Quarterly》1997,57(24):5489-5492
This investigation is part of a continuing effort to develop effective chemoprevention for carcinogenesis of the lung. The present study explores the use of aerosol administrations for this purpose. The agent selected for initial study was the synthetic glucocorticoid budesonide. This selection was based on previous work in which budesonide added to the diet was found to inhibit pulmonary adenoma formation in female A/J mice. However, high dose levels were required, i.e., of the order of 300 microg/kg, of body weight [L. W. Wattenberg and R. D. Estensen, Carcinogenesis (Lond.), 18: 2015-2017, 1997]. For aerosol administration of budesonide, a nose-only technique has been developed that entails nebulization of the compound dissolved in ethanol and subsequent stripping off of the solvent (less than 3 microl ethanol/liter of air remaining at the site of inhalation). The budesonide particles produced by the apparatus had a mass median aerodynamic diameter of less than 1 microm. An experiment has been carried out in which the inhibitory effects of aerosolized budesonide, given for 1 min six times a week, were studied. Concentrations of budesonide of 26, 81, and 148 microg/liter of air (calculated doses of 23, 72, and 126 microg/kg of body weight) were used. The aerosols were started 1 week after three oral administrations of benzo(a)pyrene (2 mg/20 g of body weight) to female A/J mice. All three doses of budesonide resulted in more than 80% inhibition of pulmonary tumor formation compared to the aerosol control and 90% or greater compared to mice not exposed to aerosol. The difference in inhibition is due to the aerosol procedure itself, which produces a reduction in tumor formation. A decrease in splenic weight (evidence of a systemic effect) occurred at all doses of budesonide. To the best of our knowledge, this is the first published effort at the use of aerosol administration to prevent neoplasia of the respiratory tract. The results of the present study show that administration of a potential chemopreventive agent by aerosol at a low dose can inhibit the occurrence of pulmonary carcinogenesis in female A/J mice. 相似文献
8.
The analysis of distributed feedback (DFB) lasers by optical low-coherence reflectometry (OLCR) shows the appearance of additional more or less periodic minima in the reflectogram. These do not reflect local minima of the reflectance, but are rather due to multipath interference effects caused by spectral filtering of the white light source by the laser under test. It is shown that the coupling coefficient of the laser can be determined from the period of these minima 相似文献
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