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A study was carried out to determine the prevalence of cervical intra-epithelial neoplasia (CIN) among women referred to Harare Hospital Colposcopy Clinic with a history of an abnormal smear. During the period, 132 patients were seen and 79 (60 pc) had colposcopic findings suggestive of CIN and 17 (13 pc) had inflammatory changes and 36 (27 pc) had normal findings. Electrocautery was used to treat low grade CIN lesions, cone biopsy was performed for higher grades of CIN and a selected group underwent hysterectomy. 相似文献
4.
The conformation of two Vicia villosa lectins specific for mannose and N-acetylgalactosamine, respectively, was studied by circular dichroism. Both showed a broad negative CD band around 220 nm and a positive one above 190 nm. CD data analysis indicated that they were rich in beta-sheet. However, they differed in conformational stability against extreme pH, at elevated temperature, and in guanidine hydrochloride and sodium dodecyl sulfate solutions. The unusual feature was that the conformation of N-acetylgalactosamine-specific lectin was virtually unaltered in 6 M guanidine hydrochloride and 7.5 mM surfactant. 相似文献
5.
SA Syed KS Zahir JR Zink O Saglaam RJ Restifo JG Thomson 《Canadian Metallurgical Quarterly》1997,38(4):396-403
Reinstatement and spontaneous recovery of previously extinguished nicotine-taking behavior were examined in rats. Male subjects were trained to self-administer nicotine (30 microg/kg per infusion, IV; one 60-min session per day for 3 weeks). Extinction sessions were then given for 5-10 days during which saline was substituted for nicotine. Subsequently, in the first set of tests for nicotine seeking, the reinstatement of lever presses that previously delivered nicotine was examined after priming injections of saline and nicotine (75, 150 and 300 microg/kg, SC; and 30 and 60 microg/kg, IV). In the second set of tests for nicotine-seeking, rats were tested after an additional 21-day drug-free period during which they were not exposed to the self-administration chambers (a test for the spontaneous recovery of drug seeking), and after priming injections of nicotine (150 and 300 microg/kg, SC). Reinstatement of extinguished food-reinforced behavior after exposure to nicotine was also determined. Priming injections of nicotine reinstated nicotine seeking regardless of the route of administration. In addition, previously extinguished nicotine seeking recovered spontaneously after a 21-day period during which rats were not exposed to the drug-taking environment. Nicotine also reinstated extinguished food-reinforced behavior in rats with a history of nicotine self-administration, but not in drug-naive rats. The present results extend previous work with opioid and stimulant drugs on reinstatement of drug seeking by the self-administered drug. It also appears that, as with other positive reinforcers, the mere passage of time is a sufficient condition for the spontaneous recovery of extinguished nicotine seeking. 相似文献
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Phage Mu transposase (A-protein) is primarily responsible for transposition of the Mu genome. The protein binds to six att sites, three at each end of Mu DNA. At most att sites interaction of a protein monomer with DNA is seen to occur over three minor and two consecutive major grooves and to result in bending up to about 90 degrees. To probe the directionality and locus of these A-protein-induced bends, we have used the antitumor antibiotic (+)-CC-1065 as a structural probe. As a consequence of binding within the minor groove, (+)-CC-1065 is able to alkylate N3 of adenine in a sequence selective manner. This selectivity is partially determined by conformational flexibility of the DNA sequence, and the covalent adduct has a bent DNA structure in which narrowing of the minor groove has occurred. Using this drug in experiments in which either gel retardation or DNA strand breakage are used to monitor the stability of the A-protein--DNA complex or the (+)-CC-1065 alkylation sites on DNA (att site L3), we have demonstrated that of the three minor grooves implicated in the interaction with A-protein, the peripheral two are 'open' or accessible to drug bonding following protein binding. These drug-bonding sites very likely represent binding at at least two A-protein-induced bending sites. Significantly, the locus of bending at these sites is spaced approximately two helical turns apart, and the bending is proposed to occur by narrowing of the minor groove of DNA. The intervening minor groove between these two peripheral sites is protected from (+)-CC-1065 alkylation. The results are discussed in reference to a proposed model for overall DNA bending in the A-protein att L3 site complex. This study illustrates the utility of (+)-CC-1065 as a probe for protein-induced bending of DNA, as well as for interactions of minor groove DNA bending proteins with DNA which may be masked in hydroxyl radical footprinting experiments. 相似文献
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J Rhodes A Dave MC Pulling RL Geggel GR Marx DR Fulton ZM Hijazi 《Canadian Metallurgical Quarterly》1998,81(10):1217-1219
Data from exercise tests, echocardiograms, and lung perfusion scans were analyzed to determine whether the excessive minute ventilation (VE) often encountered among patients with tetralogy of Fallot is due to ventilation-perfusion mismatch secondary to branch pulmonary artery stenoses. Patients with branch PA stenoses had lower peak oxygen consumptions and higher VE during exercise than did patients without stenoses, and a strong correlation existed between the degree of pulmonary blood flow maldistribution on lung perfusion scan and the amount of excessive VE during exercise. 相似文献
9.
The content and positional distribution of CLA in TAG fractions of lamb tissues was examined with either preformed CLA or
the linoleic acid precursor of CLA in the diet as experimental treatments. The CLA content of phospholipid (PL) from these
tissues was also examined. Thirteen lambs were randomized to the following dietary treatments: (i) control diet (no supplement);
(ii) CLA supplementation (0.33 g d−1 for 21 d prior to weaning) to milk-replacer of pre-ruminating lambs, or (iii) feeding linoleic acid-rich oil (6% safflower
oil on a dry matter basis) to weaned ruminating lambs. At slaughter, tissue samples were procured from diaphragm, rib muscle,
and subcutaneous (SC) adipose tissue. Safflower oil supplementation in the diet resulted in an increase in CLA content of
the TAG from diaphragm, rib muscle, and SC adipose tissue by about threefold (P<0.05) on a mol% basis. CLA was localized to the sn-1/3 positions of TAG. Animals that received pre-formed CLA, however, had increased proportions of CLA at the sn-2 position of TAG from SC adipose tissue, suggesting that there were tissue-specific dietary effects and possible age-related
effects on the mode of FA incorporation into TAG. Safflower oil supplementation in the diet had no effect on the CLA content
of PL from diaphragm, rib muscle, and SC adipose tissue, suggesting that CLA was preferentially incorporated into the TAG
of these tissues. 相似文献
10.
Mohammad Mizanur Rahman Md. Hasan Zahir Md. Eyasin Arafat Mohammad Abu Jafar Mazumder Rami Suleiman A. Madhan Kumar 《应用聚合物科学杂志》2021,138(9):49904
A waste material called oil fly ash (OFA) was acid-functionalized, yielding f-OFA-COOH, which was then reacted with cerium oxide (CeO2) to make CeO2-functionalized OFA, or f-OFA-CeO2. Pristine OFA and f-OFA-CeO2 were used to make waterborne polyurethane (WBPU) dispersions, referred to as WBPU/OFA and WBPU/f-OFA-CeO2, respectively, with defined OFA and f-OFA-CeO2 content. All the dispersions were applied to mild steel as organic coatings to evaluate their protective properties, such as their hydrophobicity, adhesive strength and UV-shielding resistance. These protective properties varied based on the OFA and f-OFA-CeO2 content. The highest water contact angle, minimum water swelling and maximum adhesive strength were found using WBPU/f-OFA-CeO2-20 coating (using 2.00 wt% f-OFA-CeO2), which also showed the maximum ultraviolet (UV) absorption via UV–vis spectroscopy analysis. This UV shielding result also matched field test results, as that coating was found to exhibit the lowest UV degradation near a marine atmosphere, as shown by X-ray photoelectron spectroscopy (XPS) analysis. The least affected hydrophobicity was also recorded for the sample with the WBPU/f-OFA-CeO2-20 coating. 相似文献