肿瘤病人皮肤成纤维细胞离体培养及放射敏感性测定

试图通过对肿瘤病人皮肤成纤维细胞的离体培养和集落形成实验,检测正常皮肤组织的放射敏感性,为探索正常组织的辐射耐受性提供理论依据。运用组织贴块法体外培养肿瘤病人皮肤成纤维细胞;采用＾１３７Ｃｓγ射线,不同剂量照射,以克隆形成法测定细胞的放射敏感性。结果表明,肿瘤病人皮肤成纤维细胞通过体外培养能够增殖传代,不同肿瘤病人皮肤成纤维细胞的放射敏感性存在明显的个体差异。     

NASOPHARYNGEAL CARCINOMA RADIOSENSITIVITY PREDICTION BY CYTOKINESIS－BLOCK MICRONUCLEUS ASSAY

ＮＡＳＯＰＨＡＲＹＮＧＥＡＬ　ＣＡＲＣＩＮＯＭＡ　ＲＡＤＩＯＳＥＮＳＩＴＩＶＩＴＹ　ＰＲＥＤＩＣＴＩＯＮ　ＢＹ　ＣＹＴＯＫＩＮＥＳＩＳ－ＢＬＯＣＫ　ＭＩＣＲＯＮＵＣＬＥＵＳ　ＡＳＳＡＹＹａｎｇＸｉｎｇ（杨星）；ＳｈｉＪｉａｎｈｕｉ（史剑慧）ａｎｄＣｈ...     

人成纤维细胞株辐射诱导凋亡与辐射敏感性相关性研究

为探讨不同放射敏感性人成纤维细胞株经辐射诱导后的凋亡情况,应用Ｔｕｎｅｌ法及流式细胞术（ＦＣＭ）检测细胞凋亡,以＾６０Ｃｏγ射线处理细胞。结果表明（１）＾６０Ｃｏγ射线照射后,集落形成实验表明,６号细胞株辐射敏感性高于８号细胞株。（２）Ｔｕｎｅｌ法和流式细胞术检测结果表明,凋亡率与辐射敏感性成正比,同样反映出６号细胞株辐射敏感性高于８号细胞株。（３）检测还发现,凋亡率与辐射剂量和辐射后时间成正比。     

NASOPHARYNGEAL CARCINOMA RADIOSENSITIVITY PREDICTION BY CYTOKINESIS—BLOCK MICRONUCLEUS ASSAY

Cytokinesis-block micronucleus method is used to evaluate the radiosensityvity of a nasopharyngeal carcinoma cell line(CNE-1) and biopsies obtained from 31 patients with nasopharyngeal carcinoma,The number of micronuclei increases with the radiation dose.A good correlation was found between the radiosensitivity determined by the micro-nucleus assay and that measured by the colony-forming assay in CNE-1 cell line(r=-0.998).Moreover,the results of micronucleus assay for tumor cells from biopsies of patients with primary carcinoma look promising for the prediction of tumor radiosensitivity.These results are encouraging but need to be confirmed with a larger number of patients with a longer follow-up.