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辐射共聚制备固定化酒精酵母细胞的研究 总被引:1,自引:0,他引:1
在低温(-78℃)条件下,用60Coγ射线对单体丙烯酸羟乙酯(HEA)与聚乙二醇二甲基丙烯酸酯系列单体14G、9G、4G、2G等进行辐射引发共聚,获得了亲水性共聚合凝胶载体,采用这些载体吸附固定化酒精酵母细胞的乙醇产率均高于游离细胞,其中Poly(HEA—14G)共聚物载体乙醇产率是游离细胞的2.45倍,12次分批反应均高于游离细胞。实验结果表明:固定化酵母细胞的乙醇生产率与共聚体系内亲水性单体及其它单体的浓度配比、双官能团单体的链长、共聚物载体的水合度以及载体结构、多孔性因素等有关,乙醇生产率的高低取决于载体的膨胀能力和多孔性. 相似文献
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In this paper, various kinds of monomers 2-hydroxyethyl methacrylate (HEMA), 2-hydroxyethyl acrylate (HEA), hydroxypropyl methacrylate (HPMA) and methoxy polyethylene glycol methylacrylate (M-23G) were eopolymerized by radiation technique at low temperature (-78℃) and several kinds of copolymer carriers were obtained. Yeast cells were immobilized through adhesion and multiplication of yeast cells themselves on these carriers. The etharol productivity of immobilized yeast cells with these carriers was related to the monomer composition and water content of copolymer carriers and the optimum monomer composition was 20%:10% in poly (HEA-M23G). In this case, the ethanol productivity of immobilized yeast cells was 26 mg /( ml·h), which was 4 times as high as that of free cells. In this study, the effect of adding of crosslinking reagent (4G) in copolymer on activity of yeast cells immobilized with the carriers were also studied. It was found that the effect of adding crosslinking reagent (4G) in lower monomer composition of poly(HEA-M23G) on the ethanol productivity of immobilized cells was better than that in higher one in this work. 相似文献
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辐射共聚制备固定化酒精酵细胞的研究 总被引:1,自引:0,他引:1
在低温(-78℃)条件下,用^60Crγ射线对单体丙烯酸羟乙酯(HEA)与聚乙二醇二甲基丙烯酸酯系列单体14G、9G、4G、2G等进行辐射引发共聚,获得了亲水性共聚合凝胶载体,采用这些载体吸附固定化酒精酵母细胞的乙醇产率均高于游离细胞,其中Poly(HEA-14G)共聚物载体乙醇产率是游离细胞的2.45倍,12次分批反应均高于游离细胞。实验结果表明:固定化酵母细胞的乙醇生产率与共聚体系内亲水性单体 相似文献
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