~(211)At对小鼠艾氏腹水癌细胞摄取嘧啶核苷的影响

~(211)At由我校1.2m回旋加速器通过核反应~(209)Bi(α,2n)~(211)At生产,实验中所用~(211)At为离子态Na~(211)At和~(211)At-Te胶体。本文运用小鼠艾氏腹水癌细胞体外培养的方法实验,结果表明不同剂量的Na~(211)At(18.5—370kBq/ml)或~(211)At-Te胶体(18.5—222kBq/ml)都能程度不等地抑制小鼠艾氏腹水癌细胞对胸苷和尿苷的摄取,从而抑制小鼠艾氏腹水癌细胞的DNA和RNA的合成;若先用Na~(211)At与小鼠艾氏腹水癌细胞作用3h,然后洗去~(211)At,再观察其对嘧啶核苷摄取的影响,发现~(211)At对核苷转运的抑制是不可逆的;此实验还揭示了~(211)At与艾氏腹水癌细胞作用的动力学过程。总之,本文实验结果对~(211)At治癌作用及机理进行了初步探讨,为进一步进行~(211)At药物和药理研究奠定了基础。     

THE INHIBITION EFFECT OF ~(211)At-Te COLLOID AND Na~(211) At INJECTIONS ON MURINE EHRLICH ASCITES TUMOR CELLS

Na211 At and 211 At-Te colloid injections are prepared. It has been demonstrated that the 211 At-Te colloid is stable in vivo and vitro, and can be applied in the study of biology and medicine. In the report, the model of Murine Ehrlich Ascites Cells cultured in vivo and vitro is elected for a series of experiments. It has been proved that Na 211At and 211At-Te colloid injections possess an inhibition effect on tumor cells. The inhibition effect was expressed in surviving of the mice and inhibiting growth of tumor as well as the changes of enzyme activity. Meanwhile, it was also noticed that Na 211 At and 211 At-Te colloid injections of various dose inhibited the absorb of pyrimidine nucleosides in Murine Ehrlich Ascites Cells. And the effect isn't reversible. It is closely related to the dose administratered and 50% inhibition rate needs about 1.48×105 Bq/ml culture.     

~(211)At的生物学效应及其药理

~(211)At作为α发射体治疗药物,其实际应用的可能性已受到广泛重视。为此,我们开展了下列工作,获得了预期的效果。