EFFECT OF CHILLING AND FREEZING ON SURVIVAL OF VIBRIO PARAHAEMOLYTICUS ON FISH FILLETS1

Vibrio parahaemolyticus is a foodborne pathogen isolated from coastal waters of the United States, and from seafoods including fish. No information is available on the viability of V. parahaemolyticus on raw, chilled and frozen fish. A three‐strain mixture of V. parahaemolyticus was inoculated on fish fillets (pH 6.4) to obtain a bacterial load of 10⁴ (high) or 10³ (low) CFU/fillet, and stored at 4C or 8C for 9 days or at – 18C for seven weeks. At 4C and 8C, and at both levels of inoculation, V. parahaemolyticus survived on the fillets for the entire duration of the study. However, there was a significant reduction (P < 0.01) in V. parahaemolyticus population on the fillets by 9 days of storage. In the frozen fillets, there was a sharp decline (P < 0.01) in the population of V. parahaemolyticus by day 5 of storage. Although chilling and freezing significantly (P < 0.01) inactivated high numbers of V. parahaemolyticus on fish, they cannot be relied upon as a method to reduce V. parahaemolyticus on fish, since the time and magnitude of reduction depends on the initial load of the pathogen and the storage temperature.     

Effect of Oxide Defect Structure on the Electrical Properties of ZrO2

The defect structure of monoclinic ZrO₂ was studied by measuring the transfer numbers and electrical conductivity as functions of O₂ pressure and temperature. The data suggest a defect structure of doubly ionized oxygen vacancies at low pressures, i.e. <10⁻¹⁹ atm, and singly ionized oxygen interstitials at pressures >10⁻⁹ atm. Zirconia is primarily an ionic conductor below #700°C and an electronic conductor at 700° to 1000°C for 10⁻²²≤P_o2≤1 atm.     

Synthesis and characterization of stable aqueous dispersions of graphene

A stable aqueous dispersion (5 mg ml^?1) of graphene was synthesized by a simple protocol based on three-step reduction of graphene oxide (GO) dispersion synthesized using the modified version of Hummers and Offeman method. Reduction of GO was carried out using sodium borohydride, hydrazine hydrate and dimethyl hydrazine as reducing agents. The chemically synthesized graphene was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–visible absorption spectroscopy, Fourier transform infrared (FTIR) and Raman spectroscopy, thermogravimetric analysis (TGA), optical microscopy. The stability of aqueous dispersions of graphene was confirmed through zeta potential measurements and the negative zeta potentials of 55–60 mV were obtained indicating the high stability of aqueous graphene dispersions.     

Preparation and characterization of PAN–KI complexed gel polymer electrolytes for solid-state battery applications

The free standing and dimensionally stable gel polymer electrolyte films of polyacrylonitrile (PAN): potassium iodide (KI) of different compositions, using ethylene carbonate as a plasticizer and dimethyl formamide as solvent, are prepared by adopting ‘solution casting technique’ and these films are examined for their conductivities. The structural, miscibility and the chemical rapport between PAN and KI are investigated using X-ray diffraction, Fourier transform infrared spectroscopy and differential scanning calorimetry methods. The conductivity is enhanced with the increase in KI concentration and temperature. The maximum conductivity at 30°C is found to be 2.089 × 10^?5 S cm^?1 for PAN:KI (70:30) wt%, which is nine orders greater than that of pure PAN (< 10^?14 S cm^?1). The conductivity-temperature dependence of these polymer electrolyte films obeys Arrhenius behaviour with activation energy ranging from 0.358 to 0.478 eV. The conducting carriers of charge transport in these polymer electrolyte films are identified by Wagner’s polarization technique and it is found that the charge transport is predominantly due to ions. The better conducting sample is used to fabricate the battery with configuration K/PAN + KI/I₂+ C + electrolyte and good discharge characteristics of battery are observed.     

A study on measuring surface deformation of the L’Aquila region using the StaMPS technique

This research compares two time-series interferometric synthetic aperture radar (InSAR) methods, namely persistent scatterer SAR interferometry (PS-InSAR) and small baseline subset (SBAS) to retrieve the deformation signal from pixels with different scattering characteristics. These approaches are used to estimate the surface deformation in the L’Aquila region in Central Italy where an earthquake of magnitude Mw 6.3 occurred on 6 April 2009. Fourteen Environmental Satellite (ENVISAT) C-band Advanced Synthetic Aperture Radar (ASAR) images, covering the pre-seismic, co-seismic, and post-seismic period, are used for the study. Both the approaches effectively extract measurement pixels and show a similar deformation pattern in which the north-west and south-east regions with respect to the earthquake epicentre show movement in opposite directions. The analysis has revealed that the PS-InSAR method extracted more number of measurement points (21,103 pixels) as compared to the SBAS method (4886 pixels). A comparison of velocity estimates shows that out of 833 common pixels in both the methods, about 62% (517 pixels) have the mean velocity difference below 3 mm year^?1 and nearly 66% pixels have difference below 5 mm year^?1. It is concluded that StaMPS-based PS-InSAR method performs better in terms of extracting more number of measurement pixels and in the estimation of mean line of sight (LOS) velocity as compared to SBAS method.     

Identification of pole-like structures from mobile lidar data of complex road environment

Pole-like structures (PLSs) located in road environment are important roadway assets. They play a vital role in road safety inspection and road planning. The use of light detection and ranging (lidar) based mobile mapping technology for mapping of PLSs is an important area of research as it holds the potential for automation. Point cloud data of rural, peri-urban, and urban road environment are used in this study, which pose special challenge in view of the complexity of terrain, unlike well-planned roads, which have been the subject of interest in existing literature for identification of PLSs. A new five-step method is proposed in this article. The first two steps, i.e. ground filtering and voxelization of filtered non-ground points, are used for data size reduction. Next three steps are used to extract PLSs from reduced data. The proposed method was tested on point cloud data of three test sites having different levels of complexities. PLSs including partially occluded pole, tilted pole, pole situated very close to other objects, and vertical pole attached to tilted pole were accurately identified. Average correctness and completeness, respectively of 92.6% and 94.9%, were achieved in three different complex test sites, i.e. urban, peri-urban, and rural sites, respectively. Computation complexity shows that our proposed method delivers fast and computationally efficient solution for identifying the PLSs from volumetric mobile lidar point cloud. Impact of PLSs on road safety and road planning is also addressed for these selected test sites.     

Quasi-cleavage fracture planes in spheroidized A533B steel

Electron backscattered diffraction (EBSD) has been used to acquire crystal orientation information around unusual microcracks induced by tensile deformation of notched specimens of spheroidized A533B steel. This unusual fracture mode has been called quasi‐cleavage and occurs at relatively low temperatures with fracture energies below that of the upper shelf. EBSD measurements on sectioned samples showed that the quasi‐cleavage cracks were intragranular. A two‐dimensional analysis technique was used in which EBSD measured crystal orientations were combined with secondary electron imaging to obtain the trace of the crack facet on the section plane. The measurements revealed that the observed crack facets were consistent with crack propagation along the {001} and {011} planes.     

Generalized probability model for estimating the proportion of the female population at different levels of fertility based on census sampling from a mixed population

The present exercise is an attempt to generalize a probability model referring to the waiting time distribution for conception in a mixed group of fecund females with two varying levels of fecundity, based on a census sample truncated at time T =t from the date of resumption of fecundity following an earlier conception (Biswas, 1975, Sankhya, Series B, 37, Part III). The generalization is made in two aspects, viz. instead of fixed conception rates, time-dependent rates decreasing with the age of the woman have been used. Additionally, unlike the model of Biswas (1975) consisting of two different fecund groups, three different levels of fecundity have been taken into consideration in this paper. Estimates of the proportion of fecund females over time, viz. π_i(t) (i = 1, 2, 3) are attempted by the method of maximum likelihood on the basis of a census sample truncated at time T = t under the generalized set up.     

Cryogenic forming behaviour of AW-6016-T4 sheet

The objective of this work is to study the cryogenic sheet metal forming behaviour of precipitation hardening AW-6016-T4. In this regard, the flow curves and forming limit curves were obtained by tension and Nakazima experimental testing methods in the temperature ranges from ?196 to 25 °C. It was found that strength and elongation increase with decreasing temperature. Small but perceived differences between microstructure of the material deformed at the room and cryogenic temperatures respectively were identified by electron backscatter diffraction (EBSD) analysis. However, no significant difference in the precipitation kinetics during continuous heating in the DSC has been observed. This study has demonstrated the potential of cryogenic forming by manufacturing a B-pillar part with 8 mm depth of side design element as compared to 6 mm at room temperature.