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1.
Paweł Jankowski Dominika Ogończyk Ladislav Derzsi Wojciech Lisowski Piotr Garstecki 《Microfluidics and nanofluidics》2013,14(5):767-774
Modification of the surfaces of polycarbonate (PC) with the use of a solution of tin (II) chloride renders them hydrophilic. The surface draping is stable against exposure to water and to alcohols. Exposure to alkanes reduces but does not diminish the effect. The method is compatible—in using the same solvent and temperature—with the hydrophobic modification of PC Jankowski et al. ( Lab Chip 11:748–752, 2011). The combination of these methods makes it possible to generate single and multiple monodisperse emulsions with the use of flow-focusing junctions in systems made in PC—material that is suitable for fabrication of multilayer, high throughput microfluidic devices. 相似文献
2.
Hydrogenases catalyze uptake and production of H2. Heterolytic cleavage of H2 bound on [NiFe]-hydrogenase (E) produces two unequal H species to form E:HaHb, where Ha and Hb behave differently. The structures of various states of the enzyme established by crystallography and spectroscopy were used to construct a catalytic cycle of the enzyme. The Ni–Fe center of the active enzyme has the Ni–Fe bridging site vacant. The enzyme is suggested to bind H2 either at Ni or Fe atom. In E:HaHb, Ha is considered to be a protein-bound hydron (proton or deuteron) at the entrance to the hydrophobic gas tunnel. The structure of a synthetic hydrogenase-mimic suggests Hb to be the 6th ligand to Fe. Two successive one-electron processes from E:HaHb complete the catalytic cycle of H2 uptake. The reverse of the cycle operates in the H2 production. The proposed catalytic cycle is consistent with the kinetic, crystallographic and spectroscopic studies. 相似文献
3.
Yasushi Mae Hideyasu Takahashi Kenichi Ohara Tomohito Takubo Tatsuo Arai 《Intelligent Service Robotics》2011,4(1):91-98
The paper presents a robot system design with highly reusable components for a component-based robot system for manipulation
tasks. The robot system is designed based on the analysis of manipulation tasks using a unified modeling language use case
diagram. For a service robot with locomotion and manipulation mechanisms, reusability of robot system components is improved
by adopting the proposed design. Our structure consists of scenario, task, robot information management server, data analyzer,
sensor hardware controller, skill, and motion hardware controller on a component-based robot system. Based on the proposed
robot system, we implemented a component-based robot system and subsequently realized a grasping motion by a service robot. 相似文献
4.
5.
Paweł Jankowski Dominika Ogończyk Ladislav Derzsi Wojciech Lisowski Piotr Garstecki 《Microfluidics and nanofluidics》2013,14(3-4):597-604
Modification of the surfaces of polycarbonate (PC) with the use of a solution of tin (II) chloride renders them hydrophilic. The surface draping is stable against exposure to water and to alcohols. Exposure to alkanes reduces but does not diminish the effect. The method is compatible—in using the same solvent and temperature—with the hydrophobic modification of PC (Jankowski et al. in Lab Chip 11:1151–1156, 2011). The combination of these methods makes it possible to generate single and multiple monodisperse emulsions with the use of flow-focusing junctions in systems made in PC—a material that is suitable for fabrication of multilayer, high-throughput microfluidic devices. 相似文献
6.
Yukari Totsuka Takashi Higuchi Toshio Imai Akiyoshi Nishikawa Takehiko Nohmi Tatsuya Kato Shuich Masuda Naohide Kinae Kyoko Hiyoshi Sayaka Ogo Masanobu Kawanishi Takashi Yagi Takamichi Ichinose Nobutaka Fukumori Masatoshi Watanabe Takashi Sugimura Keiji Wakabayashi 《Particle and fibre toxicology》2009,6(1):1-11
Background
Recently, manufactured nano/microparticles such as fullerenes (C60), carbon black (CB) and ceramic fiber are being widely used because of their desirable properties in industrial, medical and cosmetic fields. However, there are few data on these particles in mammalian mutagenesis and carcinogenesis. To examine genotoxic effects by C60, CB and kaolin, an in vitro micronuclei (MN) test was conducted with human lung cancer cell line, A549 cells. In addition, DNA damage and mutations were analyzed by in vivo assay systems using male C57BL/6J or gpt delta transgenic mice which were intratracheally instilled with single or multiple doses of 0.2 mg per animal of particles.Results
In in vitro genotoxic analysis, increased MN frequencies were observed in A549 cells treated with C60, CB and kaolin in a dose-dependent manner. These three nano/microparticles also induced DNA damage in the lungs of C57BL/6J mice measured by comet assay. Moreover, single or multiple instillations of C60 and kaolin, increased either or both of gpt and Spi- mutant frequencies in the lungs of gpt delta transgenic mice. Mutation spectra analysis showed transversions were predominant, and more than 60% of the base substitutions occurred at G:C base pairs in the gpt genes. The G:C to C:G transversion was commonly increased by these particle instillations.Conclusion
Manufactured nano/microparticles, CB, C60 and kaolin, were shown to be genotoxic in in vitro and in vivo assay systems. 相似文献7.
Youichi Ogo Kenji Nomura Hiromichi Ohta Masahiro Hirano Hideo Hosono 《Thin solid films》2006,496(1):64-69
A reactive solid-phase epitaxy (R-SPE) method combines deposition of a thick amorphous or polycrystalline layer with a desired chemical composition and post-deposition solid-phase epitaxial growth. The solid-phase epitaxial growth is invoked by thermal annealing with an assistance of a sacrificial layer working as an epitaxial template. Thereby it enables us to grow high-quality epitaxial films of complex oxides whose epitaxial films are not grown by conventional high-temperature growth techniques. It was reported that 2-nm-thick ZnO layers worked as template for growing InGaO3(ZnO)m (m = integer) epitaxial films. The present study extended the R-SPE technique to growth of various complex oxides with chemical compositions of RAO3(MO)m and to use of various epitaxial template layers. We found that mono oxide epitaxial layers such as In2O3 and Ga2O3 work as template layers as well. Alternatively, a ZnO epitaxial layer is also applicable to ZnO-free compounds. The films obtained were grown heteroepitaxially on YSZ(111) and single-crystalline when the fabrication conditions are optimized. 相似文献
8.
9.
Takahiro Kozawa Hideya Hattori Shuhei Ogo Yusuke Ide Yoshikazu Suzuki 《Journal of Materials Science》2013,48(22):7969-7973
A simple and mild synthetic route via a hydrothermal treatment of Mg2TiO4 has been developed to prepare brookite-type TiO2. The hydrothermal conversion of Mg2TiO4 to brookite proceeded in 1 M HCl solution even at 100 °C. The converted brookite grains were composed of the angular particles covered on the textured surface, leaving the original morphology of Mg2TiO4 grains. Compared with the commercially available TiO2 catalysts (rutile, anatase, and P25), the brookite obtained in this study showed a good photocatalytic activity toward the oxidation of benzyl alcohol to benzaldehyde under a simulated sunlight irradiation. 相似文献
10.
MA Torsoni RI Viana BF Barros G Stoppa M Cesquini SH Ogo 《Canadian Metallurgical Quarterly》1996,40(2):355-364
The reaction of thiol reagents with G. carbonaria hemoglobin was studied, and the oxygen equilibrium and kinetic of oxidation of derivatives determined. The oxygen affinity and kinetic of oxidation of hemoglobin derivatives were modified to various extents depending on the nature of thiol reagents used. Diamide yielded approximately 80% polymeric hemoglobin, although the oxidation kinetic, and the functional properties, were practically invariant (T1/2 = 10.0 min.; P50 = 5.0 mm Hg at pH 7.4; alkaline Bohr effect = -0.64). Iodoacetamide did not modify the electrophoretic pattern significantly, although all the free SH groups of hemoglobin were alkylated. A P50 of 2.5 mmHg at pH 7.4 and the Bohr effect of -0.15 were obtained; the T1/2 of about 6.4 min. was shorter than that for un-modified Hb. Similar T1/2 were obtained for Hb treated with oxidized glutathione, which produced polymeric Hb and glutathionyl-Hb. The oxygen binding characteristics showed that both of Hb derivatives, glutathionyl-Hb and polymeric Hb, maintain the capacity to transport the gas. 相似文献