Rewired Cellular Metabolic Profiles in Response to Metformin under Different Oxygen and Nutrient Conditions

Metformin is a metabolic disruptor, and its efficacy and effects on metabolic profiles under different oxygen and nutrient conditions remain unclear. Therefore, the present study examined the effects of metformin on cell growth, the metabolic activities and consumption of glucose, glutamine, and pyruvate, and the intracellular ratio of nicotinamide adenine dinucleotide (NAD⁺) and reduced nicotinamide adenine dinucleotide (NADH) under normoxic (21% O₂) and hypoxic (1% O₂) conditions. The efficacy of metformin with nutrient removal from culture media was also investigated. The results obtained show that the efficacy of metformin was closely associated with cell types and environmental factors. Acute exposure to metformin had no effect on lactate production from glucose, glutamine, or pyruvate, whereas long-term exposure to metformin increased the consumption of glucose and pyruvate and the production of lactate in the culture media of HeLa and HaCaT cells as well as the metabolic activity of glucose. The NAD⁺/NADH ratio decreased during growth with metformin regardless of its efficacy. Furthermore, the inhibitory effects of metformin were enhanced in all cell lines following the removal of glucose or pyruvate from culture media. Collectively, the present results reveal that metformin efficacy may be regulated by oxygen conditions and nutrient availability, and indicate the potential of the metabolic switch induced by metformin as combinational therapy.     

Enhanced Light Storage of SrAl2O4 Glass‐Ceramics Controlled by Selective Europium Reduction

A luminescent Eu, Dy: SrAl₂O₄ glass‐ceramics with high transparency in the visible region was successfully synthesized using the frozen sorbet technique with the control of O₂ partial pressure () for the oxidation of Eu²⁺ ions. The glass‐ceramics include Eu²⁺, Eu³⁺, and Dy³⁺ ions, and thus exhibits three characteristic types of emission bands, 4f–5d at around 520 nm (Eu²⁺ ions), 4f–4f at 610 nm (Eu³⁺ ions), and 480 nm (Dy³⁺ ions). The Eu, Dy: SrAl₂O₄ glass‐ceramics provide remarkable long‐persistent luminescence under dark condition. The glass‐ceramics also exhibits color‐changing luminescence in the visible region based on their remarkable light storage properties. The luminescent Eu, Dy: SrAl₂O₄ glass‐ceramics using the frozen sorbet technique with control of are promising materials for application in novel photonic and light storage materials.     

Biosynthesis of novel thermoplastic polythioesters by engineered Escherichia coli

The development of non-petrochemical sources for the plastics industry continues to progress as large multinationals focus on renewable resources to replace fossil carbon. Many bacteria are known to accumulate polyoxoesters as water-insoluble granules in the cytoplasm. The thermoplastic and/or elastomeric behaviour of these biodegradable polymers holds promise for the development of various technological applications. Here, we report the synthesis and characterization of microbial polythioesters (PTEs), a novel class of biopolymers of general technological relevance. Biosynthesis of PTE homopolymers was achieved using a recombinant strain of Escherichia coli that expressed a non-natural pathway consisting of a butyrate kinase, a phosphotransbutyrylase, and a PHA synthase. Different homopolymers were produced, consisting of either 3-mercaptopropionate, 3-mercaptobutyrate, or 3-mercaptovalerate repeating units, if the respective mercaptoalkanoic acids were provided as precursor substrates to the fermentative process. The PTEs contributed up to 30% (w/w) of the cellular dry weight and were identified as hydrophobic inclusions in the cytoplasm. The chemical and stereochemical homogeneity of the purified PTEs were identified by different methods, and the estimated physical properties were compared to the oxypolyester equivalents, revealing low crystalline order and, for the poly(3-mercaptopropionate) improved thermal stability. The ability to produce PTEs through a biosynthetic route opens up new avenues in the field of biomaterials.     

EACLE : Energy-Aware Clustering Scheme with Transmission Power Control for Sensor Networks

In this paper, we propose a new energy efficient clustering scheme with transmission power control named “EACLE” (Energy-Aware CLustering scheme with transmission power control for sEnsor networks) for wireless sensor networks, which are composed of the following three components; “EACLE clustering” is a distributed clustering method by means of transmission power control, “EACLE routing” builds a tree rooted at a sink node and sets the paths from sensor nodes taking energy saving into consideration, and “EACLE transmission timing control” changes the transmission timing with different levels of transmission power to avoid packet collisions and facilitates packet binding. With an indoor wireless channel model which we obtained from channel measurement campaigns in rooms and corridors and an energy consumption model which we obtained from a measurement of a chipset, we performed computer simulations to investigate the performance of EACLE in a realistic environment. Our simulation results indicate that EACLE outperforms a conventional scheme such as EAD (Energy-Aware Data-centric routing) in terms of communication success rate and energy consumption. Furthermore, we fully discuss the impact of transmission power and timing control on the performance of EACLE.     

A new desk-top encounter-type haptic device with an actively driven pen-tablet LCD panel

In conventional haptic devices for virtual reality (VR) systems, a user interacts with a scene by handling a tool (such as a pen) using a mechanical device (i.e. an end-effector-type haptic device). In the case that the device can ‘mimic’ a VR object, the user can interact directly with the VR object without the mechanical constraint of a device (i.e. an encounter-type haptic device). A new challenge of an encounter-type haptic device is displaying the visuals and haptic information simultaneously on a single device. We are proposing a new desk-top encounter-type haptic device with an actively driven pen-tablet LCD panel. The proposed device is capable of providing pseudo-3D visuals and haptic information on a single device. As the result, the system provides to the user a sense of interaction with a real object. To develop a proof-of-concept prototype, a compact parallel mechanism was developed and implemented. The aim of this research is to propose a new concept in haptic research. In this paper, the concept, the prototype, and some preliminary evaluation tests with the proposed system are presented.     

Experimental study of cell pattern formation induced by internal heat sources in a horizontal fluid layer

This study determines the flow structure in a convection cell with an internally heated layer by PIV to elucidate the convection cell transition mechanisms. The vertical velocity component is determined and the cell behaviour with respect to Rayleigh number is investigated quantitatively. Cell expansion process is described as a consequence of development of the descending flow at the centre of cells. The results suggest that a spoke-like structure is stable in this system in ideal conditions and a double-cell structure is formed when there are restrictions on the system, i.e. finite lateral boundaries.     

工程光学的发展趋势

过去,光学对物理学的发展起了非常重要的作用。现在,由于与光学相关的环境的重大改善,即激光的出现、光电子学与计算机的发展,使光学或工程光学的应用迅猛发展。本文综合工程光学中的一些进展以总结它的新近发展趋势。     

Development of a new model for representing elastoplastic deformation and fracture behaviors of solid fats

A new simulation model, namely, ADEM Ductile Fracture model (ADF model) is developed to represent elastoplastic deformation and fracture behaviors of solid fats. Experimental measurement of the coefficient of plastic starting distance and the yield point affecting the deformation behavior has been obtained by compression test. The fracture distance affecting the fracture behavior has been measured by cutting test. The ADF model shows a good match with the experimentally determined compression and cutting behaviors of the solid fats, and load–strain curves as a function of plate thickness. This confirms that the ADF model could represent the elastoplastic deformation and fracture behaviors of the solid fats.     

Toward tunable and bright deep‐red persistent luminescence of Cr3+ in garnets

Garnet structure (A₃B₂C₃O₁₂) with three different cation sites is a very flexible host material widely used for w‐LEDs, solid‐state lasers, scintillators, and so on. In this work, we have successfully developed six different Cr³⁺‐doped garnets: Y₃Ga_4.99Cr_0.01O₁₂ (YGG:Cr), Gd₃Ga_4.99Cr_0.01O₁₂ (GGG:Cr), Lu₃Ga_4.99Cr_0.01O₁₂ (LuGG:Cr), Y₃Sc_1.99Cr_0.01Ga₃O₁₂ (YSGG:Cr), Gd₃Sc_1.99Cr_0.01Ga₃O₁₂ (GSGG:Cr), and Lu₃Sc_1.99Cr_0.01Ga₃O₁₂ (LuSGG:Cr), which exhibit persistent luminescence (PersL) due to Cr³⁺ emission matching well with both the response curve of the Si detector and the wavelength region of the first biological window (NIR‐I, 650‐950 nm). The main emission band of Cr³⁺ in these garnets can be easily tunable from the sharp R‐line emission due to the ²E (²G)→⁴A₂ (⁴F) transition in the strong crystal field to the broad band emission due to the ⁴T₂ (⁴F)→⁴A₂ (⁴F) transition in the weak one when Lu³⁺ in the A site and Ga³⁺ in the B site are, respectively, replaced by larger cations, Y³⁺/Gd³⁺ and Sc³⁺. Furthermore, based on the knowledge of 4f energy levels of 15 lanthanide ions in the host‐referred binding energy (HRBE) diagram, we took the GSGG host as a typical example to discuss the feasibility of four trivalent lanthanides (Sm³⁺, Eu³⁺, Tm³⁺, Yb³⁺) as potential sensitizers for enhancing Cr³⁺ PersL.