Fuzzy logic for signal prediction in nuclear systems

In this paper, we present a fuzzy logic-based algorithm which allows to build a predictive model of an evolving signal. The if-then rules are inferred from the available input-output data. The errors of the model predictions with respect to the measured plant signals can then be used to detect, in real-time, any deviation of the process due either to a component's failure or to an operator's action. Applications of the approach are presented with respect to a chaotic time series of literature and to the water level in the steam generator of a PWR.     

Biohybrid Bovine Bone Matrix for Controlled Release of Mesenchymal Stem/Stromal Cell Lyosecretome: A Device for Bone Regeneration

SmartBone^® (SB) is a biohybrid bone substitute advantageously proposed as a class III medical device for bone regeneration in reconstructive surgeries (oral, maxillofacial, orthopedic, and oncology). In the present study, a new strategy to improve SB osteoinductivity was developed. SB scaffolds were loaded with lyosecretome, a freeze-dried formulation of mesenchymal stem cell (MSC)-secretome, containing proteins and extracellular vesicles (EVs). Lyosecretome-loaded SB scaffolds (SBlyo) were prepared using an absorption method. A burst release of proteins and EVs (38% and 50% after 30 min, respectively) was observed, and then proteins were released more slowly with respect to EVs, most likely because they more strongly adsorbed onto the SB surface. In vitro tests were conducted using adipose tissue-derived stromal vascular fraction (SVF) plated on SB or SBlyo. After 14 days, significant cell proliferation improvement was observed on SBlyo with respect to SB, where cells filled the cavities between the native trabeculae. On SB, on the other hand, the process was still present, but tissue formation was less organized at 60 days. On both scaffolds, cells differentiated into osteoblasts and were able to mineralize after 60 days. Nonetheless, SBlyo showed a higher expression of osteoblast markers and a higher quantity of newly formed trabeculae than SB alone. The quantification analysis of the newly formed mineralized tissue and the immunohistochemical studies demonstrated that SBlyo induces bone formation more effectively. This osteoinductive effect is likely due to the osteogenic factors present in the lyosecretome, such as fibronectin, alpha-2-macroglobulin, apolipoprotein A, and TGF-β.     

Multidrug Idebenone/Naproxen Co-loaded Aspasomes for Significant in vivo Anti-inflammatory Activity

The use of proper nanocarriers for dermal and transdermal delivery of anti-inflammatory drugs recently gained several attentions in the scientific community because they pass intact and accumulate payloads in the deepest layers of skin tissue. Ascorbyl palmitate-based vesicles (aspasomes) can be considered a promising nanocarrier for dermal and transdermal delivery due to their skin whitening properties and suitable delivery of payloads through the skin. The aim of this study was the synthesis of multidrug Idebenone/naproxen co-loaded aspasomes for the development of an effective anti-inflammatory nanomedicine. Aspasomes had suitable physicochemical properties and were safe in vivo if topically applied on human healthy volunteers. Idebenone/naproxen co-loaded aspasomes demonstrated an increased therapeutic efficacy of payloads compared to the commercially available Naprosyn® gel, with a rapid decrease of chemical-induced erythema on human volunteers. These promising results strongly suggested a potential application of Idebenone/naproxen multidrug aspasomes for the development of an effective skin anti-inflammatory therapy.     

The BDNF/TrkB Neurotrophin System in the Sensory Organs of Zebrafish

The brain-derived neurotrophic factor (BDNF) was discovered in the last century, and identified as a member of the neurotrophin family. BDNF shares approximately 50% of its amino acid with other neurotrophins such as NGF, NT-3 and NT-4/5, and its linear amino acid sequences in zebrafish (Danio rerio) and human are 91% identical. BDNF functions can be mediated by two categories of receptors: p75NTR and Trk. Intriguingly, BDNF receptors were highly conserved in the process of evolution, as were the other NTs’ receptors. In this review, we update current knowledge about the distribution and functions of the BDNF-TrkB system in the sensory organs of zebrafish. In fish, particularly in zebrafish, the distribution and functions of BDNF and TrkB in the brain have been widely studied. Both components of the system, associated or segregated, are also present outside the central nervous system, especially in sensory organs including the inner ear, lateral line system, retina, taste buds and olfactory epithelium.     

R&D,capital structure and ownership concentration: evidence from Italian microdata

The purpose of the article is to provide some evidence on the interconnection between capital structure, R&D investment and ownership concentration using a unique panel data-set of Italian firms. We study the effect of R&D intensity on leverage for two groups of firms which are different in terms of their degree of ownership concentration. Our results suggest for Public Limited Companies, a nonlinear relationship between R&D intensity and leverage, with the latter first increasing and then decreasing. Interestingly, the same result is not found to hold true for Private Limited Companies, which are characterized by a more concentrated ownership.     

A Monte Carlo methodological approach to plant availability modeling with maintenance, aging and obsolescence

In this paper we present a Monte Carlo approach for the evaluation of plant maintenance strategies and operating procedures under economic constraints. The proposed Monte Carlo simulation model provides a flexible tool which enables one to describe many of the relevant aspects for plant management and operation such as aging, repair, obsolescence, renovation, which are not easily captured by analytical models. The maintenance periods are varied with the age of the components. Aging is described by means of a modified Brown–Proschan model of imperfect (deteriorating) repair which accounts for the increased proneness to failure of a component after it has been repaired. A model of obsolescence is introduced to evaluate the convenience of substituting a failed component with a new, improved one. The economic constraint is formalized in terms of an energy, or cost, function; optimization studies are then performed using the maintenance period as the control parameter.     

An efficient Monte Carlo-SubSampling approach to first passage problems

In the realm of the Continuous Time Random Walk (CTRW) and in conjunction with the Monte Carlo (MC) approach, we consider the transport of a chemical or radioactive pollutant in a 3D heterogeneous medium, focusing on the first passage time (FPT), defined as the time required by the walkers representative of the dangerous particles to travel within the medium until crossing a target disk, thus entering another medium which should instead remain clean, such as a water well or an aquifer. The advantage of the MC approach is the possibility of simulating different features of the travel such as different waiting-time probabilities, space dependent jump lengths, absorption and desorption phenomena, Galilei invariant and variant velocities driven by external forces. When the computer time required for collecting a suitable number of target crossings is excessive, we propound to hybridize the MC approach with the recent SubSampling computational procedure, usefully applied in the engineering reliability field to computing very small failure probabilities in short computer time. To tackle the FPT problem we iteratively consider groups of a few thousands of walkers: in each iteration we select a fraction of them closer to the target, ignoring the remaining ones, and then restore the group by creating with the MC technique new walkers even more close to the target. The successive groups have large conditional probabilities which can be estimated one after the other in short time and whose product yields the total probability. By so doing the FPT can be actually computed in much shorter times: we report examples of Gaussian and anomalous distributions in which the reduction with respect to a pure MC computation is of orders of magnitude.