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排序方式: 共有78条查询结果,搜索用时 15 毫秒
1.
Fernanda Arboite de Oliveira Ana Paula Pasqualotto Wladimir Padilha da Silva Eduardo Cesar Tondo 《Food research international (Ottawa, Ont.)》2012,45(2):1000-1003
This study aimed to characterize Salmonella Enteritidis (SE) isolated from blood (n = 12) and feces (n = 68) of salmonellosis victims in Southern Brazil. All isolates were submitted to antimicrobial susceptibility testing, PCR-ribotyping, and XbaI macrorestriction Pulsed-Field Gel Eletrophoresis (PFGE). Results demonstrated high levels of ampicillin and nalidixic acid resistance, and strains isolated in different geographic regions were clustered together, presenting a common resistance profile. All strains demonstrated similar and related PCR-ribotyping patterns (R1, R2, and R3); being that the predominant profile R1 grouped 47.5% of the strains. PFGE profile P1 grouped the majority of the strains (96.25%), suggesting a clonal relationship among the strains or inability of molecular typing methods to discriminate strains of this serovar. Results suggested on an increase in antimicrobial resistance and that strains of S. Enteritidis with similar PFGE and PCR-ribotyping profiles were involved in several salmonellosis outbreaks in Southern Brazil. 相似文献
2.
Liana Franco Padilha Cristine Carretoni Ferreira Fabricio Machado Márcio Nele José Carlos Pinto 《应用聚合物科学杂志》2014,131(9)
In this work, NIRS is employed to provide compositions of α‐olefin copolymers, allowing for evaluation of the effects of certain key process variables on the final NIR spectral responses of obtained polymer materials. This work also introduces a new temperature programmed analytical technique, which combines NIRS measurements with partial fractionation of α‐olefin copolymers. The new proposed technique can be used for evaluation of polyolefin compositions, as presented here for poly(propene/1‐butene) copolymers. Besides, preliminary results obtained from thermal fractionation experiments indicate that this new proposed experimental technique can be employed for characterization of comonomer sequence distributions of α‐olefin copolymers. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40127. 相似文献
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4.
Mayra A. D. Saleh Renato C. F. Neves Paula M. Moraes Paula M. Lima Felipe A. dos Santos Fábio A. Silva Pedro M. Padilha 《Sensing and Instrumentation for Food Quality and Safety》2009,3(2):108-113
This paper proposes a method to determine iron in samples of fish feed and feces using ultrasound in the extraction of the
analyte and in subsequent quantification by flame atomic absorption spectrometry. Using HCl 0.10 mol L−1 as the extraction solution, the optimal conditions of extraction were found to be: granulometry of the sample <60 μm; a sonication
time of five cycles of 10 s and sonication power of 136 W. The method was applied in studies of the availability of iron in
four food sources used in the diet of Nile Tilapia. The results obtained with the proposed extraction method allowed us to
calculate the coefficients of apparent digestibility of iron in the food sources, which was not possible when using results
obtained from samples mineralized by acid digestion. 相似文献
5.
Ferromagnetic stainless steels (SSs) have been investigated as potential candidates for dental prosthesis applications in replacement of magnetic attachments made of noble and expensive alloys. In order to be used as biomaterials, their corrosion resistance has to be appropriate. The corrosion resistance of passive materials is related to the characteristics of the passive film formed and their properties might be investigated by electrochemical impedance spectroscopy (EIS), potentiodynamic polarization curves, and Mott–Schottky approach. The corrosion resistance and the capacitance of the passive films formed on PM2000, DIN 1.4575, and 17‐4 PH advanced SSs during 2 days of immersion in a phosphate buffered saline solution (PBS), at 25 °C and pH 7.0 were studied by electrochemical techniques. A commercial alloy Dyna EFM was also analyzed for comparison reasons. The results showed that films on tested materials behave as both n‐type and p‐type semiconductors and the PM2000 presented the highest corrosion resistance among all of them. 相似文献
6.
Grain subdivision and recrystallization in oligocrystalline tantalum during cold swaging and subsequent annealing 总被引:1,自引:0,他引:1
Hugo R. Z. Sandim Angelo F. Padilha Valerie Randle Wolfgang Blum 《International Journal of Refractory Metals and Hard Materials》1999,17(6):431-435
A coarse-grained ingot of high-purity tantalum was deformed by swaging at room temperature to a strain of 1.28. During annealing at 900°C for 30 min two neighboring grains were observed to behave quite differently. Electron backscattering diffraction (EBSD) results show noticeable differences in terms of the misorientations developed in both grains. The grain developing larger misorientations recrystallized much more readily than the other. The result is interpreted in terms of the differences in grain subdivision into strongly misoriented regions. 相似文献
7.
Mariane Severino Mohammed Gagaoua Welder Baldassini Richard Ribeiro Juliana Torrecilhas Guilherme Pereira Rogrio Curi Luis Artur Chardulo Pedro Padilha Otvio Machado Neto 《International journal of molecular sciences》2022,23(20)
Proteomics has been widely used to study muscle biology and meat quality traits from different species including beef. Beef proteomics studies allow a better understanding of the biological processes related to meat quality trait determination. This study aimed to decipher by means of two-dimensional electrophoresis (2D-PAGE), mass spectrometry and bioinformatics the changes in post-mortem muscle with a focus on proteins differentially expressed in the Longissimus thoracis (LT) muscle of immunocastrated young heifers and steers. Carcass traits, chemical composition, pH, instrumental color (L*, a*, b*), cooking loss and Warner-Bratzler shear force (WBSF) of meat from F1 Montana-Nellore cattle were also evaluated. Backfat thickness (BFT) and intramuscular fat content (IMF) were 46.8% and 63.6% higher in heifers (p < 0.05), respectively, while evaporation losses (EL) were 10.22% lower compared to steers. No differences (p > 0.05) were observed for tenderness evaluated by WBSF (3, 10, and 17 days post-mortem), pH, and color traits (L*, a* and b*) between the experimental groups. The study revealed several proteins to be differentially expressed proteins in heifers compared steers (p < 0.05). In heifers, proteins involved in nutrient transport (TF, ALB, and MB), energy metabolism (ALDOA, GAPDH, and PKM), and oxidative stress and response to stress (HSPA8 and CA3) were associated with a greater BFT and IMF deposition. The higher expression of these proteins indicated greater oxidative capacity and lower glycolytic activity in the LT muscle of heifers. In steers, there was greater abundance of protein expression related to muscle contraction and proteins of structure (ACTA1, TPM2 and TNNT3), energy metabolism (ENO1, ENO3, PYGM, PGM1 and TPI1) and ATP metabolism (ATP5F1B, PEBP1 and AK1), indicating greater glycogenolysis in LT muscle, suggesting a shift in the glycolytic/oxidative fibers of steers. 相似文献
8.
The effects of niobium addition on a completely austenitic stainless steel matrix consisting of Fe-15%Cr-15%Ni, with about 0, 0.5, 1 and 2% niobium, were studied by determination of stacking fault energies by means of X-ray diffraction analysis. A strong influence of niobium on stacking-fault energies was found, whose behaviour can be explained as being due to decrease in the electron/atom ratio and increase in the L. Pauling electron vacancy number of austenitic Fe-Cr-Ni matrix brought on by niobium. 相似文献
9.
10.
Maysa Terada Angelo Jos�� de Oliveira Zimmermann Hugo Ricardo Zschommler Sandim Isolda Costa Angelo Fernando Padilha 《Journal of Applied Electrochemistry》2011,41(8):951-959
In the present work, the corrosion resistance of ferritic-martensitic EUROFER 97 and ODS-EUROFER steels was tested in solutions containing NaCl or H2SO4 and KSCN, both at 25 °C. The results were compared to those of AISI 430 ferritic and AISI 410 martensitic conventional stainless steels. The as-received samples were tested by electrochemical techniques, specifically, electrochemical impedance spectroscopy, potentiodynamic polarization curves, and double-loop electrochemical potentiokinetic reactivation tests. The surfaces were observed by scanning electron microscopy after exposure to corrosive media. The results showed that EUROFER 97 and ODS-EUROFER alloys present similar corrosion resistance but lower than ferritic AISI 430 and martensitic 410 stainless steels. 相似文献