Surveillance after colorectal cancer surgery

Early diagnosis of local and distant recurrences of colorectal cancer remains difficult and there is no agreement on the effectiveness of follow-up in these patients. The aim of this study is to assess the value of our method of follow-up. We consider 239 patients with colorectal cancer and at least 2 years follow-up following radical resection. A local recurrence appeared in 26 patients (10.9%), a distant metastasis in 41 (17.1%), while in seven (2.9%) local and distant recurrences appeared simultaneously. Local recurrence was detected because of an increase in carcinoembryonic antigen (CEA) level in 15 patients (57.7%), during a scheduled endoscopy in four (15.4%) and because of symptoms in seven (26.9%). In seven patients (26.9%) a radical resection was possible. Distant metastases were detected by CEA levels in 20 patients (48.8%), by ultrasonography (U.S.) in 12 (29.3%) and by chest X-ray in five (12.2%). In 13 of 26 patients with liver metastases a resection was performed. This study shows that few patients benefit from follow-up and only CEA levels and liver U.S. performed intensively between 15 and 36 months after surgery are useful in early detection of recurrences. A modification of the follow-up to the single patient, according to the stage, location and grading of cancer, could improve the results, so lowering the costs of this expensive practice.     

Temperature Oscillation Calorimetry: Robustness analysis of different algorithms for the evaluation of the heat transfer coefficient

Applicability of different temperature oscillation calorimetry algorithms, previously proposed in the literature, for the evaluation of the heat transfer coefficient during the course of the reaction, is analysed by referring to the experimental data from a methyl methacrylate polymerisation reactor.Results are coincident in the nominal case (no errors on data), while differences appear in the elaboration of real data. The effect of uncertainty in the experimental data has been simulated in terms of error on the amplitude and on the phase of reactor and jacket temperature profiles.This approach allows to indicate algorithms having the best robustness properties and to give an explanation of phase errors in terms of a lumped parameter which accounts for different phenomena, not included in the basic hypotheses of the method. Based on this observation, a procedure to eliminate this source of errors from experimental data is proposed for the more general algorithm presented to handle oscillating temperature profiles generated by different techniques.     

Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins

Gap junctions are collections of intercellular channels composed of structural proteins called connexins (Cx). We have examined the functional interactions of the three rodent connexins present in the lens, Cx43, Cx46, and Cx50, by expressing them in paired Xenopus oocytes. Homotypic channels containing Cx43, Cx46, or Cx50 all developed high conductance. heterotypic channels composed of Cx46 paired with either Cx43 or Cx50 were also well coupled, whereas Cx50 did not form functional channels with Cx43. We also examined the functional response of homotypic and heterotypic channels to transjunctional voltage and cytoplasmic acidification. We show that all lens connexins exhibited sensitivity to cytoplasmic acidification as well as to voltage, and that voltage-dependent closure of heterotypic channels for a given connexin was dramatically influenced by its partner connexins in the adjacent cell. Based on the observation that Cx43 can discriminate between Cx46 and Cx50, we investigated the molecular determinants that specify compatibility by constructing chimeric connexins from portions of Cx46 and Cx50 and testing them for their ability to form channels with Cx43. When the second extracellular (E2) domain in Cx46 was replaced with the E2 of Cx50, the resulting chimera could no longer form heterotypic channels with Cx43. A reciprocal chimera, where the E2 of Cx46 was inserted into Cx50, acquired the ability to functionally interact with Cx43. Together, these results demonstrate that formation of intercellular channels is a selective process dependent on the identity of the connexins expressed in adjacent cells, and that the second extracellular domain is a determinant of heterotypic compatibility between connexins.     

A Web‐based Open‐source Geoinformation Tool for Regional Water Resources Assessment

Water Resources Management - To reduce the impact of droughts and increase the resilience of regional water systems, various competing demands, such as hydropower, supply, irrigation and river...     

THROO: a Tracked Hybrid Rover to Overpass Obstacles

In this paper, the design, simulation and experimental tests are presented for THROO: a Tracked Hybrid Rover, which has been developed to Overpass Obstacles. The proposed mobile robot has 3-DOFs and it is capable of straight motion, turning ability and two operations, namely rover-like motion with tracks on flat terrain and walking-like motion with track and legs to overpass obstacles to move on uneven terrain. The leg mechanism is composed of a four-bar linkage, which has been synthesized according to the desired features. In particular, the Burmester problem, which aims at finding the geometric parameters of a four-bar linkage required for a prescribed set of finitely separated poses are solved for the case understudy. Dynamic simulations have been carried out and a prototype has been built. The proposed results show the hybrid rover ability to overpass obstacles, for which size is comparable or greater than the track high.     

Feeding response of Ips paraconfusus to phloem and phloem metabolites of Heterobasidion annosum-inoculated ponderosa pine,Pinus ponderosa

In studies of feeding by the bark beetle, Ips paraconfusus, two pine stilbenes (pinosylvin and pinosylvin methyl ether), ferulic acid glucoside, and enantiomers of the four most common sugars present in ponderosa pine phloem (sucrose, glucose, fructose, and raffinose) did not stimulate or reduce male feeding when assayed on wet -cellulose with or without stimulatory phloem extractives present. When allowed to feed on wet -cellulose containing sequential extracts (hexane, methanol, and water) of ponderosa pine phloem, methanol and water extractives stimulated feeding, but hexane extractives did not. Males confined in wet -cellulose containing aqueous or organic extracts of culture broths derived from phloem tissue and containing the root pathogen, Heterobasidion annosum, ingested less substrate than beetles confined to control preparations. In an assay using logs from uninoculated ponderosa pines, the mean lengths of phloem in the digestive tracts increased as time spent feeding increased. Males confined to the phloem of basal logs cut from ponderosa pines artificially inoculated with H. annosum ingested significantly less phloem than beetles in logs cut from trees that were (combined) mock-inoculated or uninoculated and did not contain the pathogen. However, individual pathogen-containing treatments were not significantly different from uninoculated controls. It was concluded that altered feeding rates are not a major factor which may explain why diseased ponderosa pines are colonized by I. paraconfusus.     

On sets of numbers accepted by P/T systems composed by join

Recently, some studies linked the computational power of abstract computing systems based on multiset rewriting to models of Petri nets and the computation power of these nets to their topology. In turn, the computational power of these abstract computing devices can be understood by just looking at their topology, that is, information flow.Here we continue this line of research by introducing J languages and proving that they can be accepted by place/transition systems whose underlying net is composed only by join. Moreover, we study how J languages relate to other families of formal languages.     

The role of spectral resolution and classifier complexity in the analysis of hyperspectral images of forest areas

Remote sensing hyperspectral sensors are important and powerful instruments for addressing classification problems in complex forest scenarios, as they allow one a detailed characterization of the spectral behavior of the considered information classes. However, the processing of hyperspectral data is particularly complex both from a theoretical viewpoint [e.g. problems related to the Hughes phenomenon (Hughes, 1968) and from a computational perspective. Despite many previous investigations that have been presented in the literature on feature reduction and feature extraction in hyperspectral data, only a few studies have analyzed the role of spectral resolution on the classification accuracy in different application domains. In this paper, we present an empirical study aimed at understanding the relationship among spectral resolution, classifier complexity, and classification accuracy obtained with hyperspectral sensors for the classification of forest areas. We considered two different test sets characterized by images acquired by an AISA Eagle sensor over 126 bands with a spectral resolution of 4.6 nm, and we subsequently degraded its spectral resolution to 9.2, 13.8, 18.4, 23, 27.6, 32.2 and 36.8 nm. A series of classification experiments were carried out with bands at each of the degraded spectral resolutions, and bands selected with a feature selection algorithm at the highest spectral resolution (4.6 nm). The classification experiments were carried out with three different classifiers: Support Vector Machine, Gaussian Maximum Likelihood with Leave-One-Out-Covariance estimator, and Linear Discriminant Analysis. From the experimental results, important conclusions can be made about the choice of the spectral resolution of hyperspectral sensors as applied to forest areas, also in relation to the complexity of the adopted classification methodology. The outcome of these experiments are also applicable in terms of directing the user towards a more efficient use of the current instruments (e.g. programming of the spectral channels to be acquired) and classification techniques in forest applications, as well as in the design of future hyperspectral sensors.     

Functional Redesign of Mantis 2.0, a Hybrid Leg-Wheel Robot for Surveillance and Inspection

The paper discusses the redesign of the second version of the Mantis hybrid leg-wheel mobile robot, conceived for surveillance and inspection tasks in unstructured indoor and outdoor environments. This small-scale ground mobile robot is characterized by a main body equipped with two front actuated wheels, a passive rear axle and two rotating legs. Motion on flat and even ground is purely wheeled in order to obtain high speed, high energetic efficiency and stable camera vision; only in case of obstacles or ground irregularities the front legs realize a mixed leg-wheel locomotion to increase the robot climbing ability; in particular, the outer profile of the legs, inspired by the praying mantis, is specially designed to climb square steps. The multibody simulations and the experimental tests on the first prototype have shown the effectiveness of the mixed leg-wheel locomotion not only for step climbing, but also on uneven and yielding terrains. Nevertheless, extensive experimental tests have shown that the front wheels may slip in the last phase of step climbing in case of contact with some materials. In order to overcome this problem, the leg design has been modified with the introduction of auxiliary passive wheels, which reduce friction between legs and step upper surface; these wheels are connected to the legs by one-way bearings, in order to rotate only when they are pulled by the front wheels, and remaining locked when they have to push forward the robot. The influence of the auxiliary wheels on the front wheels slippage is investigated by means of theoretical analysis and multibody simulations.