全文获取类型
收费全文 | 198篇 |
免费 | 13篇 |
专业分类
综合类 | 1篇 |
化学工业 | 71篇 |
金属工艺 | 7篇 |
机械仪表 | 6篇 |
建筑科学 | 5篇 |
能源动力 | 15篇 |
轻工业 | 43篇 |
石油天然气 | 2篇 |
无线电 | 5篇 |
一般工业技术 | 22篇 |
冶金工业 | 4篇 |
原子能技术 | 1篇 |
自动化技术 | 29篇 |
出版年
2024年 | 1篇 |
2023年 | 3篇 |
2022年 | 14篇 |
2021年 | 19篇 |
2020年 | 10篇 |
2019年 | 15篇 |
2018年 | 5篇 |
2017年 | 2篇 |
2016年 | 14篇 |
2015年 | 3篇 |
2014年 | 9篇 |
2013年 | 17篇 |
2012年 | 21篇 |
2011年 | 18篇 |
2010年 | 7篇 |
2009年 | 14篇 |
2008年 | 9篇 |
2007年 | 4篇 |
2006年 | 7篇 |
2004年 | 3篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1996年 | 4篇 |
1995年 | 1篇 |
1990年 | 1篇 |
排序方式: 共有211条查询结果,搜索用时 15 毫秒
1.
Sanja Ramljak Matthias Schmitz Cendrine Repond Inga Zerr Luc Pellerin 《International journal of molecular sciences》2021,22(4)
The effect of a cellular prion protein (PrPc) deficiency on neuroenergetics was primarily analyzed via surveying the expression of genes specifically involved in lactate/pyruvate metabolism, such as monocarboxylate transporters (MCT1, MCT2, MCT4). The aim of the present study was to elucidate a potential involvement of PrPc in the regulation of energy metabolism in different brain regions. By using quantitative real-time polymerase chain reaction (qRT-PCR), we observed a marked reduction in MCT1 mRNA expression in the cortex of symptomatic Zürich I Prnp−/− mice, as compared to their wild-type (WT) counterparts. MCT1 downregulation in the cortex was accompanied with significantly decreased expression of the MCT1 functional interplayer, the Na+/K+ ATPase α2 subunit. Conversely, the MCT1 mRNA level was significantly raised in the cerebellum of Prnp−/− vs. WT control group, without a substantial change in the Na+/K+ ATPase α2 subunit expression. To validate the observed mRNA findings, we confirmed the observed change in MCT1 mRNA expression level in the cortex at the protein level. MCT4, highly expressed in tissues that rely on glycolysis as an energy source, exhibited a significant reduction in the hippocampus of Prnp−/− vs. WT mice. The present study demonstrates that a lack of PrPc leads to altered MCT1 and MCT4 mRNA/protein expression in different brain regions of Prnp−/− vs. WT mice. Our findings provide evidence that PrPc might affect the monocarboxylate intercellular transport, which needs to be confirmed in further studies. 相似文献
2.
Validation of plant part measurements using a 3D reconstruction method suitable for high-throughput seedling phenotyping 总被引:2,自引:0,他引:2
Franck Golbach Gert Kootstra Sanja Damjanovic Gerwoud Otten Rick van de Zedde 《Machine Vision and Applications》2016,27(5):663-680
In plant phenotyping, there is a demand for high-throughput, non-destructive systems that can accurately analyse various plant traits by measuring features such as plant volume, leaf area, and stem length. Existing vision-based systems either focus on speed using 2D imaging, which is consequently inaccurate, or on accuracy using time-consuming 3D methods. In this paper, we present a computer-vision system for seedling phenotyping that combines best of both approaches by utilizing a fast three-dimensional (3D) reconstruction method. We developed image processing methods for the identification and segmentation of plant organs (stem and leaf) from the 3D plant model. Various measurements of plant features such as plant volume, leaf area, and stem length are estimated based on these plant segments. We evaluate the accuracy of our system by comparing the measurements of our methods with ground truth measurements obtained destructively by hand. The results indicate that the proposed system is very promising. 相似文献
3.
This paper deals with the problem of optimization of job sequence in a two-machine flow shop problem in the presence of uncertainty.
It is assumed that the processing times of jobs on the machines are described by triangular fuzzy sets. A new optimization
algorithm based on Johnson”s algorithm for deterministic processing times and on an improvement of McCahon and Lee”s algorithm
is developed and presented. In order to compare fuzzy processing times, McCahon and Lee use mean values of their corresponding
fuzzy sets. It is shown that this approach cannot fully explore possible relationships between fuzzy sets. In order to overcome
this drawback we consider different fuzzy sets determined by λ-cuts of the fuzzy processing times. Extensive experiments show
that the new algorithm gives better solutions with respect to makespan than existing McCahon and Lee's algorithm. 相似文献
4.
In recent years the processing speed of computers has increased dramatically. This in turn has allowed search algorithms to execute more iterations in a given amount of real-time. Does this necessarily always lead to an improvement in the quality of final solutions? This paper is devoted to the investigation of that question. We present two variants of local search where the search time can be set as an input parameter. These two approaches are: a time-predefined variant of simulated annealing and an adaptation of the “great deluge” method. We present a comprehensive series of experiments which show that these approaches significantly outperform the previous best results (in terms of solution quality) on a range of benchmark exam timetabling problems. Of course, there is a price to pay for such better results: increased execution time. We discuss the impact of this trade-off between quality and execution time. In particular we discuss issues involving the proper estimation of the algorithm's execution time and the assessment of its importance. 相似文献
5.
A multi-agent architecture for dynamic scheduling of steel hot rolling 总被引:13,自引:0,他引:13
Peter I. Cowling Djamila Ouelhadj Sanja Petrovic 《Journal of Intelligent Manufacturing》2003,14(5):457-470
Steel production is a complex process and finding coherent and effective schedules for the wide variety of production steps, in a dynamic environment, is a challenging task. In this paper, we propose a multi-agent architecture for integrated dynamic scheduling of the hot strip mill (HSM) and the continuous caster. The scheduling systems of these processes have very different objectives and constraints, and operate in an environment where there is a substantial quantity of real-time information concerning production failures and customer requests. Each process is assigned to an agent which independently, seeks an optimal dynamic schedule at a local level taking into account local objectives, real-time information and information received from other agents. Each agent can react to real-time events in order to fix any problems that occur. We focus here, particularly, on the HSM agent which uses a tabu search heuristic to create good predictive–reactive schedules quickly. The other agents simulate the production of the coil orders and the real-time events, which occur during the scheduling process. When real-time events occur on the HSM, the HSM agent might decide whether to repair the current schedule or reschedule from scratch. To address this problem, a range of schedule repair and complete rescheduling strategies are investigated and their performance is assessed with respect to measures of utility, stability and robustness, using an experimental simulation framework. 相似文献
6.
Bojana Radojković Bore Jegdić Jovanka Pejić Dunja Marunkić Anđela Simović Sanja Eraković Pantović 《工业材料与腐蚀》2024,75(4):444-459
The influence of welding current and nitrogen content in argon shielding gas on the resistance of the welded joint (weld metal and heat-affected zone [HAZ]) of the stainless steel X5CrNi18-10 to the formation and growth of pits was investigated. Also, the susceptibility of the welded joint to intergranular corrosion was examined. Pitting corrosion resistance indicators were determined based on anodic potentiodynamic polarization measurements in NaCl + Na2SO4 solution, while susceptibility to intergranular corrosion was determined by the potentiokinetic method with a double loop (DL EPR). SEM/EDS was used to analyze the microstructure. It has been shown that higher nitrogen content in shielding gas leads to an increase in the resistance of welded joints (weld metal and HAZ) to the pit formation. However, an improvement in the resistance to the pit formation leads to a decline in the resistance of the welded joint to pit growth. An explanation of this phenomenon is proposed. Also, it was shown that the increase of the welding current increases the susceptibility of the welded joint to intergranular corrosion, while the higher nitrogen content has no effect. 相似文献
7.
Gorica Vuković Deyana Shtereva Vojislava Bursić Rositsa Mladenova Sanja Lazić 《LWT》2012,49(2):312-319
Babies and small children are especially sensitive population to the exposure to environmental contaminants. Their small mass and developing systems, including brain development may show adverse health effects from even low levels of contamination on a chronic or single dose case. In this paper one extraction method and two chromatographic techniques for the determination of pesticide residues in baby food were evaluated. A liquid chromatography–tandem mass spectrometry technique combined with electrospray ionization (ESI), (LC–MS/MS) and gas chromatography–mass spectrometry detection (GC–MSD) technique were applied in the detection of 50 pesticides in baby food. So-called QuEChERS (quick, easy, cheap, effective, rugged and safe) method was used as a sample preparation procedure. The recoveries were investigated at three levels (5, 10 and 50 μg/kg) and the results obtained showed compliance with the contemporary EU requirements with a few exceptions. LOQs for most of the tested pesticides were below the EU MRLs (10 μg/kg), except deltamethrin, cypermethrin, fenvalerate, phosalone and beta-cyfluthrin (LOQs were 10 μg/kg). Both techniques were applied in the analysis of 50 samples of baby food manufactured in Serbia. 相似文献
8.
Dr. Robert Kuhnert Dr. Lydia Kuhnert Dr. Menyhárt-B. Sárosi Sven George Dr. Dijana Draca Dr. Svetlana Paskas Dr. Bettina Hofmann Prof. Dr. Dieter Steinhilber Prof. Dr. Walther Honscha Prof. Dr. Sanja Mijatović Prof. Dr. Danijela Maksimović-Ivanić Prof. Dr. Evamarie Hey-Hawkins 《ChemMedChem》2022,17(1):e202100588
12-Lipoxygenase is crucial for tumour angiogenesis. 5,6,7-Trihydroxy-2-phenyl-4H-1-benzopyran-4-one (baicalein) is a suitable inhibitor for this enzyme but is rapidly metabolised in vivo. Thus, an improvement of the metabolic stability is necessary to enhance the therapeutic efficiency. An emerging approach to enhance metabolic stability of carbon-based pharmaceuticals is the use of metabolically stable, non-toxic boron clusters, such as dicarba-closo-dodecaborane(12)s (carboranes) as phenyl mimetics. Therefore, the unsubstituted phenyl ring of baicalein was replaced by meta-carborane, resulting in borcalein, the carborane analogue of baicalein. This substitution resulted in a decreased inhibitory activity toward 12-lipoxygenase, but led to increased toxicity in melanoma (A375, B16, B16F10) and colon cancer cell lines (SW480, HCT116, CT26CL25) with decreased tumour selectivity in comparison to baicalein. Surprisingly, borcalein displays a different mechanism of cytotoxicity with increased intracellular production of reactive oxygen species (ROS), reactive nitrogen species (RNS) and nitric oxide (NO). 相似文献
9.
Dipeptidyl peptides III (DPP III) is a dual-domain zinc exopeptidase that hydrolyzes peptides of varying sequence and size. Despite attempts to elucidate and narrow down the broad substrate-specificity of DPP III, there is no explanation as to why some of them, such as tynorphin (VVYPW), the truncated form of the endogenous heptapeptide spinorphin, are the slow-reacting substrates of DPP III compared to others, such as Leu-enkephalin. Using quantum molecular mechanics calculations followed by various molecular dynamics techniques, we describe for the first time the entire catalytic cycle of human DPP III, providing theoretical insight into the inhibitory mechanism of tynorphin. The chemical step of peptide bond hydrolysis and the substrate binding to the active site of the enzyme and release of the product were described for DPP III in complex with tynorphin and Leu-enkephalin and their products. We found that tynorphin is cleaved by the same reaction mechanism determined for Leu-enkephalin. More importantly, we showed that the product stabilization and regeneration of the enzyme, but not the nucleophilic attack of the catalytic water molecule and inversion at the nitrogen atom of the cleavable peptide bond, correspond to the rate-determining steps of the overall catalytic cycle of the enzyme. 相似文献
10.
Sanja Dogramadzi Maria Elena Giannaccini Christopher Harper Mohammad Sobhani Roger Woodman Jiyeon Choung 《Journal of Intelligent and Robotic Systems》2014,76(1):73-117
Robot manufacturers will be required to demonstrate objectively that all reasonably foreseeable hazards have been identified in any robotic product design that is to be marketed commercially. This is problematic for autonomous mobile robots because conventional methods, which have been developed for automatic systems do not assist safety analysts in identifying non-mission interactions with environmental features that are not directly associated with the robot’s design mission, and which may comprise the majority of the required tasks of autonomous robots. In this paper we develop a new variant of preliminary hazard analysis that is explicitly aimed at identifying non-mission interactions by means of new sets of guidewords not normally found in existing variants. We develop the required features of the method and describe its application to several small trials conducted at Bristol Robotics Laboratory in the 2011–2012 period. 相似文献