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1.
Urska Vrhovsek Lara Giongo Fulvio Mattivi Roberto Viola 《European Food Research and Technology》2008,226(4):817-824
This study describes the first application of a new procedure for acid hydrolysis of Rubus ellagitannins in methanol, which allows quantification of all the major reaction products (ellagic acid, methyl-sanguisorboate,
methyl gallate and an unknown ellagic acid derivative) and estimation of the mean degree of polymerization (mDP) of Rubus ellagitannins. The ellagitannins of 21 raspberry and 15 blackberry cultivars produced in Trentino, Italy during 2005 were
extracted and characterised. The total content of ellagitannins was shown to vary by a factor of 2 among raspberry cultivars
and by a factor of 4 among blackberry cultivars. 相似文献
2.
Bobnar V Erste A Gradisar U Filipic C Levstik A Kutnjak Z 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2011,58(11):2270-2275
It has long been considered that polar nanoregions in relaxors form at Burns temperature T(d) ≈ 600K. High-temperature dielectric investigations of Pb(Mg(1/3)Nb(2/3)) O(3) (PMN) single crystal, PMN-PbTiO(3) ceramics, and (Pb,La) (Zr,Ti)O(3) ceramics reveal, however, that dielectric dispersion, detected around 600K, is due to the Maxwell-Wagner-type contributions of surface layers. The intrinsic response was analyzed in terms of the universal scaling, taking into account the asymptotic and the correction-to-scaling behavior, and the results imply much higher T(d) or formation of polar nanoregions in a broad temperature range. High values of the dielectric constant indicate, however, that polar order already exists at the highest measured temperatures of 800K. The obtained critical exponents indicate critical behavior associated with universality classes typically found in spin glasses. 相似文献
3.
Monika Savarin Urska Kamensek Katarina Znidar Vesna Todorovic Gregor Sersa Maja Cemazar 《International journal of molecular sciences》2021,22(6)
Targeting tumor vasculature through specific endothelial cell markers represents a promising approach for cancer treatment. Here our aim was to construct an antibiotic resistance gene-free plasmid encoding shRNAs to simultaneously target two endothelial cell markers, CD105 and CD146, and to test its functionality and therapeutic potential in vitro when delivered by gene electrotransfer (GET) and combined with irradiation (IR). Functionality of the plasmid was evaluated by determining the silencing of the targeted genes using qRT-PCR. Antiproliferative and antiangiogenic effects were determined by the cytotoxicity assay tube formation assay and wound healing assay in murine endothelial cells 2H-11. The functionality of the plasmid construct was also evaluated in malignant melanoma tumor cell line B16F10. Additionally, potential activation of immune response was measured by induction of DNA sensor STING and proinflammatory cytokines by qRT-PCR in endothelial cells 2H-11. We demonstrated that the plasmid construction was successful and can efficiently silence the expression of the two targeted genes. As a consequence of silencing, reduced migration rate and angiogenic potential was confirmed in 2H-11 endothelial cells. Furthermore, induction of DNA sensor STING and proinflammatory cytokines were determined, which could add to the therapeutic effectiveness when used in vivo. To conclude, we successfully constructed a novel plasmid DNA with two shRNAs, which holds a great promise for further in vivo testing. 相似文献
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5.
Eliana Fortes Gris Fulvio Mattivi Eduardo Antonio Ferreira Urska Vrhovsek Rozangela Curi Pedrosa Marilde T. Bordignon-Luiz 《Food chemistry》2011
The free flavan-3-ol and proanthocyanidin (PA) profile and the antioxidant capacity of wines Vitis vinifera L., 2006 and 2007 vintages, from the São Joaquim region, at southern Brazil, are reported here for the first time. Catechin and epicatechin were the two main monomers in the wine samples, followed by gallocatechin and epigallocatechin; and the PA B1 was the main dimer. The terminal units of the PAs were constituted mainly by catechin units, with the co-presence of epicatechin, gallocatechin, epigallocatechin and traces of epicatechin gallate. The epicatechin and epigallocatechin units were the main constituents of the extension units of PAs with the co-presence of catechin and epicatechin gallate. The values for the mean degree of polymerisation ranged from 4.9 to 9.8. The wine samples demonstrated effective scavenging activity against DPPH and ABTS radicals and against lipid peroxidation in vitro. A positive correlation existed between flavan-3-ol content and antioxidant capacity in vitro. 相似文献
6.
Danijela VUJOEVI Uro CVELBAR Urka REPNIK Martina MODIC Saa LAZOVI Tina ZAVANIK-BERGANT Nevena PUA Boban MUGOA Evangelos GOGOLIDES Zoran Lj PETROVI Miran MOZETI 《等离子体科学和技术》2017,19(7)
The degradation of Escherichia coli bacteria by treatment with cold,weakly ionised,highly dissociated oxygen plasma,with an electron temperature of 3 eV,a plasma density of 8 × 1015 m-3 and a neutral oxygen atom density of 3.5 × 1021 m-3 was studied.To determine the 'real' plasma effects,two methods were used for evaluation and determination,as well as a comparison of the number of bacteria that had survived:the standard plate count technique (PCT) and advanced fluorescence-activated cell sorting (FACS).Bacteria were deposited onto glass substrates and kept below 50 ℃ during the experiments with oxygen plasma.The results showed that the bacteria had fully degraded after about 2 min of plasma treatment,depending slightly on the amount of bacteria that had been deposited on the substrates.The very precise determination of the O flux on the substrates and the two-method comparison allowed for the determination of the critical dose of oxygen atoms required for the destruction of a bacterial cell wall-about 6 × 1024 m-2-as well as deactivation of the substrates-about 8 × 1025 m-2.These results were taken in order to discuss other results obtained by comparable studies and scientific method evaluations in the determination of plasma effects on bacteria. 相似文献
7.
The degradation of?Escherichia coli?bacteria?by treatment with?cold,?weakly ionised, highly dissociated oxygen plasma,?with?an?electron temperature of 3 e V, a?plasma density of 8?×?10~(15)m~(-3) and a?neutral oxygen atom density of 3.5?×?1021m~(-3) was studied. To determine the ‘real' plasma effects,?two methods were used for evaluation and determination, as well as a comparison of the number of?bacteria that had?survived: the standard plate count technique(PCT) and?advanced fluorescence-activated cell sorting(FACS). Bacteria were deposited onto glass substrates and kept below 50 °C during the experiments with oxygen plasma. The results showed that the?bacteria had?fully degraded after about 2 min of plasma treatment, depending slightly on the amount of bacteria that had been?deposited on the substrates. The very?precise determination of the O flux on?the substrates and the two-method comparison allowed for the determination of the critical dose of oxygen atoms required for the destruction of a bacterial cell wall—about 6?×?10~(24)m~(-2)—as well as deactivation of the substrates—about 8?×?1025m~(-2).These results were taken in order to discuss other results obtained by comparable studies and scientific method?evaluations in the determination of plasma effects on bacteria. 相似文献
8.
正坎皮拉公司办公楼由天然石材筑成,大厅宽敞,内设办公室和行政区域,安保仓库配有运输区、储藏区。我们把坎皮拉公司办公楼做了大规模翻修,采用高质量、具代表性内部固定设施。仓库是一座混凝土独立建筑,半开放式设计尤为引人注目,出于为仓储考虑,屋顶上的灯倾斜设置,天花板则形似漂浮在这些灯上一样,整栋建筑看起来似乎没有封闭,却充满活力。 相似文献
9.
Marija Smiljkovic Dr. Minos‐Timotheos Matsoukas Dr. Eftichia Kritsi Dr. Urska Zelenko Dr. Simona Golic Grdadolnik Dr. Ricardo C. Calhelha Dr. Isabel C. F. R. Ferreira Dr. Snezana Sankovic‐Babic Dr. Jasmina Glamoclija Dr. Theano Fotopoulou Dr. Maria Koufaki Dr. Panagiotis Zoumpoulakis Dr. Marina Sokovic 《ChemMedChem》2018,13(3):251-258
Four heteroaromatic compounds bearing nitrate esters were selected using a virtual‐screening procedure as putative sterol 14α‐demethylase (CYP51) Candida albicans inhibitors. Compounds were examined for their inhibition on C. albicans growth and biofilm formation as well as for their toxicity. NMR spectroscopy studies, in silico docking, and molecular dynamics simulations were used to investigate further the selectivity of these compounds to fungal CYP51. All compounds exhibited good antimicrobial properties, indicated with low minimal inhibitory concentrations and ability to inhibit formation of fungal biofilm. Moreover, all of the compounds had the ability to inhibit growth of C. albicans cells. N‐(2‐Nitrooxyethyl)‐1Η‐indole‐2‐carboxamide was the only compound with selectivity on C. albicans CYP51 that did not exhibit cytotoxic effect on cells isolated from liver and should be further investigated for selective application in new leads for the treatment of candidiasis. 相似文献