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排序方式: 共有149条查询结果,搜索用时 15 毫秒
1.
Yuta Uenoyama Atsushi Matsuda Kazune Ohashi Koji Ueda Misaki Yokoyama Takuya Kyoutou Kouji Kishi Youichi Takahama Masaaki Nagai Takaaki Ohbayashi Osamu Hotta Hideki Matsuzaki 《International journal of molecular sciences》2022,23(9)
Aberrant glycosylation of IgA1 is involved in the development of IgA nephropathy (IgAN). There are many reports of IgAN markers focusing on the glycoform of IgA1. None have been clinically applied as a routine test. In this study, we established an automated sandwich immunoassay system for detecting aberrant glycosylated IgA1, using Wisteria floribunda agglutinin (WFA) and anti-IgA1 monoclonal antibody. The diagnostic performance as an IgAN marker was evaluated. The usefulness of WFA for immunoassays was investigated by lectin microarray. A reliable standard for quantitative immunoassay measurements was designed by modifying a purified IgA1 substrate. A validation study using multiple serum specimens was performed using the established WFA-antibody sandwich automated immunoassay. Lectin microarray results showed that WFA specifically recognized N-glycans of agglutinated IgA1 in IgAN patients. The constructed IgA1 standard exhibited a wide dynamic range and high reactivity. In the validation study, serum WFA-reactive IgA1 (WFA+-IgA1) differed significantly between healthy control subjects and IgAN patients. The findings indicate that WFA is a suitable lectin that specifically targets abnormal agglutinated IgA1 in serum. We also describe an automated immunoassay system for detecting WFA+-IgA1, focusing on N-glycans. 相似文献
2.
Kenji Imai Koji Takai Tatsunori Hanai Makoto Shiraki Yusuke Suzuki Hideki Hayashi Takafumi Naiki Youichi Nishigaki Eiichi Tomita Masahito Shimizu Hisataka Moriwaki 《International journal of molecular sciences》2014,15(7):11294-11306
Obesity-related metabolic abnormalities, including adipokine imbalance and chronic inflammation, are involved in liver carcinogenesis. Chemerin, a novel adipokine, plays a critical role in adipogenesis, energy metabolism, and inflammation. We evaluated the impact of serum chemerin levels on liver functional reserves in hepatocellular carcinoma (HCC) patients and on the recurrence and prognosis of HCC. This study included 44 patients with any stage of HCC who underwent curative treatment at Gifu Municipal Hospital (Gifu, Japan) between 2006 and 2007. Recurrence-free survival and overall survival were estimated using the Kaplan-Meier method. Serum albumin levels (Pearson’s correlation coefficient; r = 0.3110, p = 0.0399), platelet counts (r = 0.4159, p = 0.0050), and prothrombin times (r = 0.3775, p = 0.0115) were significantly correlated with serum chemerin levels in patients with HCC, and they were inversely correlated with Child-Pugh scores (r = −0.3732, p = 0.0126), serum alanine aminotransferase levels (r = −0.3864, p = 0.0105), and total bilirubin levels (r = −0.4023, p = 0.0068). Among these variables, a multiple comparison test identified that platelet counts and total bilirubin levels were associated with serum chemerin levels (p < 0.0083). No significant correlation was found between serum chemerin levels and recurrence-free survival (p = 0.3691) or overall survival (p = 0.7916). In HCC patients, serum chemerin concentrations were correlated with liver functional reserves and platelet counts, but not with recurrence or prognosis. 相似文献
3.
4.
Moriwaki H Takagi Y Tanaka M Tsuruho K Okitsu K Maeda Y 《Environmental science & technology》2005,39(9):3388-3392
Perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) are shown to be globally distributed, environmentally persistent, and bioaccumulative. Although the toxicities of these compounds were reported, the cleanup procedure from the environment is not developed because of their inertness. In this report the sonochemical degradations of PFOS and PFOA to the products through the fission of the perfluorocarbon chains were observed and the half-life times of the PFOS and PFOA degradations under an argon atmosphere determined to be 43 and 22 min, respectively. The shortening of perfluorocarbon chain of PFOS and PFOA leads to the lowering of the toxicity in view of the decrease of the persistence, and the technique would contribute to the remediation of the environmental pollution by these compounds. 相似文献
5.
Keisuke Kobayashi Minoru Ryu Yutaka Doshida Youichi Mizuno Clive A. Randall 《Journal of the American Ceramic Society》2013,96(2):531-537
The (1?x)NaNbO3–(x)NaTaO3 solid solution was investigated for x ≤ 0.4 in terms of new high‐temperature and high‐permittivity dielectric system that is suitable for base metal inner electrode capacitor applications. The addition of Ta significantly enhanced the resistivity of the dielectric, resulting in superior resistivity than the dielectrics‐formulated BaTiO3 systems that dominate the multilayer ceramic capacitor dielectric devices. The voltage dependence of the permittivity was also superior to BaTiO3‐based materials, providing higher capacitance at higher temperatures. A transmission electron microscopy study illustrated that the grains had so‐called core‐shell structure. According to the electron diffraction analysis, the core region had an inhomogeneous structure between antiferroelectric and ferroelectric phases, and shell region had an incommensurate ferroelectric‐like structure. The core and shell region had Nb‐ and Ta‐rich composition, respectively, and their interface was compositionally sharp, implying that shell region was formed via a liquid phase during the sintering process with an incongruent Ta dissolution reprecipitation. We anticipate that these or similar materials based on the alkali‐niobate perovskites can be further enhanced to provide capacitor solutions from 150°C to 250°C, which is an important range for a number of new AC–DC invertor and engine control units. 相似文献
6.
7.
Chie Ando Toshimasa Suzuki Youichi Mizuno Hiroshi Kishi Sayuri Nakayama Mamoru Senna 《Journal of Materials Science》2008,43(18):6182-6192
In an attempt to obtain finest possible microparticles of BaTiO3 (BT) with highest possible tetragonality via a solid state route, starting mixtures comprising BaCO3 and TiO2 were subjected to various pretreatments including addition of glycine and mechanical activation. Reaction processes were
monitored by the changes in the weight, crystallinity, and morphology in detail. While mechanical activation with glycine
significantly increased the rate of reaction and homogeneity of the particle size of the product, BT, simultaneous particle
growth of BT was intolerably acute for micro-electronic devices. The fast particle coarsening was predominated by the coalescence
of BT tiny particles formed around titania. A mixture with higher homogeneity was attained by using finer starting materials
under wet mixing, avoiding significant mechanical stressing. Particle growth of BT was suppressed to ca. 100 nm to obtain
fully crystallized BT particles without significant loss of tetragonality and, hence, close to meet our requirements for MLCCs. 相似文献
8.
Yutaka Tokuda Youichi Nagae Hitoshi Sakane Jyoji Ito 《Journal of Electronic Materials》2010,39(6):719-722
Defect production behavior in hydrogen-implanted n-type silicon has been studied by varying the implantation temperature from 88 K to 303 K. Deep-level transient spectroscopy
has been used to reveal electron trap spectra for Schottky diodes fabricated at room temperature after implantation. Metastable
defects are observed in addition to vacancy- and hydrogen-related defects. It is found that the production rates of these
defects are greatly enhanced by hydrogen implantation near 270 K. It is suggested that hydrogen plays an important role in
enhancement of defect production rates, since such defect production behavior is not observed in He-implanted samples. 相似文献
9.
Yasuo Watanabe Masahiro Yamaguchi Jun Sakamoto Youichi Tamai 《Yeast (Chichester, England)》1993,9(3):213-220
Plasma membrane was isolated from the salt-tolerant yeast Candida versatilis and the ATPase in plasma membrane was characterized. The ATPase was a typical H+-ATPase with similar properties to the Saccharomyces cerevisiae and Zygosaccharomyces rouxii enzymes. It was reacted with antibody (IgG) raised against S. cerevisiae plasma membrane H+-ATPase. The ATPase activity was not changed by adding NaCl and KCl to the assay solutions, but was increased by NH, especially by ammonium sulfate. In vivo stimulation of ATPase activity was observed by the addition of NaCl into the culture medium, as observed in Z. rouxii. No in vivo activation of H+-ATPase by glucose metabolism was observed in C. versatilis cells and the activity was independent of the growth phase, like Z. rouxii and unlike S. cerevisiae cells. 相似文献
10.
Takashi Isoshima Youichi Okabayashi Eisuke Ito Masahiko Hara Whee Won Chin Jin Wook Han 《Organic Electronics》2013,14(8):1988-1991
Negative giant surface potential was realized in a vacuum-evaporated film of tris(7-propyl-8-hydroxyquinolinolato) aluminum(III) [Al(7-Prq)3]. Electroabsorption response of the film presented an inverted polarity to that of tris(8-hydroxyquinolinolato) aluminum (Alq3), suggesting opposite noncentrosymmetry of molecular orientation. Asymmetric dice model with molecular geometric effect has been proposed, and propyl substitution at 7 position of the ligands was indicated to affects the molecular posture on the surface to invert the polarity of noncentrosymmetry. Our results opened a new possibility of controlling molecular orientation in a film for device applications. 相似文献