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排序方式: 共有274条查询结果,搜索用时 31 毫秒
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Every general practitioner has to deal with urologic emergencies. The most frequent illnesses are urinary retention, acute scrotum, priapism, macrohematuria, nephritic colic, obstructive pyelonephritis and pyonephrosis. Whereas urinary retention, as well as acute ureteric stone colic must generally be treated by the practitioner, the urologist must often be consulted in case of an acute scrotum or for priapism. Testicular torsion is one situation, where surgical treatment needs to be performed within 6 hours. Of utmost importance is his timely assistance with the obstructive pyelonephritis and pyonephrosis. These are initially often not recognized, especially because the first ultrasound examination of the intrarenal pyelone may not show a dilatation of the collecting system despite obstruction. If the adequate treatment with drainage and antibiotics is applied too late, this can result in serious and potentially lethal consequences. 相似文献
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Thomas M. Ackermann Dr. Lars Allmendinger Dr. Georg Höfner Prof. Dr. Klaus T. Wanner 《ChemMedChem》2021,16(1):199-215
This study describes the first binding assay for glycine transporter 2 (GlyT2) following the concept of MS Binding Assays. The selective GlyT2 inhibitor Org25543 was employed as a reporter ligand and it was quantified with a highly sensitive and rapid LC-ESI-MS/MS method. Binding of Org25543 at GlyT2 was characterized in kinetic and saturation experiments with an off-rate of 7.07×10−3 s−1, an on-rate of 1.01×106 M−1 s−1, and an equilibrium dissociation constant of 7.45 nM. Furthermore, the inhibitory constants of 19 GlyT ligands were determined in competition experiments. The validity of the GlyT2 affinities determined with the binding assay was examined by a comparison with published inhibitory potencies from various functional assays. With the capability for affinity determination towards GlyT2 the developed MS Binding Assays provide the first tool for affinity profiling of potential ligands and it represents a valuable new alternative to functional assays addressing GlyT2. 相似文献
4.
Zhen EY Berna MJ Jin Z Pritt ML Watson DE Ackermann BL Hale JE 《Proteomics. Clinical applications》2007,1(7):661-671
Heart fatty acid binding protein (Fabp3) is a cytosolic protein expressed primarily in heart, and to a lesser extent in skeletal muscle, brain, and kidney. During myocardial injury, the Fabp3 level in serum is elevated rapidly, making it an ideal early marker for myocardial infarction. In this study, an MS‐based selected reaction monitoring method (LC‐SRM) was developed for quantifying Fabp3 in rat serum. Fabp3 was enriched first through an immobilized antibody, and the protein was digested on beads directly. A marker peptide of Fabp3 was quantified using LC‐SRM with a stable isotope‐labeled peptide standard. For six quality control samples with Fabp3 ranging from 0.256 to 25 ng, the average recovery following the procedure was about 73%, and the precision (%CV) between replicates was less than 7%. The Fabp3 concentrations in rat serum peaked 1 h after isoproterenol treatment, and returned to baseline levels 24 h after the dose. Elevated Fabp3 levels were also detected in rats administered with a PPAR α/δ agonist, which has shown to cause skeletal muscle necrosis. Fabp3 can be used as a biomarker for both cardiac and skeletal necroses. The cross‐validation of the LC‐SRM method with an existing ELISA method is described. 相似文献
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J. Ackermann C. Videlot T.N. Nguyen L. Wang P.M. Sarro F. Fages 《Advanced materials (Deerfield Beach, Fla.)》2004,16(19):1709-1712
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Quiberoni A Tremblay D Ackermann HW Moineau S Reinheimer JA 《Journal of dairy science》2006,89(10):3791-3799
Phage infections still represent a serious risk to the dairy industry, in which Streptococcus thermophilus is used in starter cultures for the manufacture of yogurt and cheese. The goal of the present study was to analyze the biodiversity of the virulent S. thermophilus phage population in one Argentinean cheese plant. Ten distinct S. thermophilus phages were isolated from cheese whey samples collected in a 2-mo survey. They were then characterized by their morphology, host range, and restriction patterns. These phages were also classified within the 2 main groups of S. thermophilus phages (cos- and pac-type) using a newly adapted multiplex PCR method. Six phages were classified as cos-type phages, whereas the 4 others belonged to the pac-type group. This study illustrates the phage diversity that can be found in one factory that rotates several cultures of S. thermophilus. Limiting the number of starter cultures is likely to reduce phage biodiversity within a fermentation facility. 相似文献
8.
Ackermann T. Ancell G. Borup L.D. Eriksen P.B. Ernst B. Groome F. Lange M. Mohrlen C. Orths A.G. O'Sullivan J. de la Torre M. 《Power and Energy Magazine, IEEE》2009,7(6):65-75
The power systems in Denmark, Spain, Ireland, and New Zealand have some of the highest wind penetrations in the world (see Table 1). The management of the different power systems to date, with increasing amounts of wind energy, has been successful. There have been no incidents in which the wind has directly or indirectly been a major factor causing operational problems for the system. However, there are a number of parameters that are being monitored that indicate the need for active management in the near future (and in some cases already today). In this article, we briefly describe the situations in these four countries, giving special emphasis to the market integration of wind power, the use of wind forecasting, and curtailment experience. The final section provides an overview of the main wind forecasting methodologies and challenges. 相似文献
9.
G. Fechner H. Ackermann und B. Lexow 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1969,140(3):145-149
Zusammenfassung Es wird über eine kombinierte dünnschichtchromatographisch-enzymatische Methode zur Bestimmung von Rückständen in der Milch nach Dimethoatbehandlung von Milchkühen beschrieben. Die Nachweisgrenze liegt für Dimethoat bei 0,02–0,04 mg/l und für PO-Dimethoat bei 0,002–0,004 mg/l.Untersuchungen an lactierenden Rindern nach dem Aufgieß-Verfahren einer 4%igen Dimethoat-Formulierung für die Bekämpfung der Dasselfliegenlarve beim Rind zeigten relativ hohe Rückstandswerte an Dimethoat und seinem PO-Isologen in den folgenden Gemelken an, so daß für den genannten Bereich dieser Wirkstoff nicht empfohlen werden kann.
Determination of dimethoate and PO-dimethoate in milk by thinlayer-chromatographie
Summary A combined thinlayer-chromatographic and enzymatic method is described to analyse residues in milk from cows treated with dimethoate (0.0—dimethyl-S-(N-methyl-carbamoylmethyl)dithio-phosphate). The detection limit for dimethoate is between 0,02–0,04 mg/l and for PO-dimethoate between 0,002–0,004 mg/l.Investigations with lactating cows showed relatively high residue values for dimethoate and its PO-isologues in milk so that these substances cannot be recommended for warble fly control measurements.相似文献
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