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Jean-Charles Créput Abderrafiaâ Koukam 《Soft Computing - A Fusion of Foundations, Methodologies and Applications》2008,12(11):1125-1141
The paper presents an extension of the self- organizing map (SOM) by embedding it into an evolutionary algorithm to solve
the Vehicle Routing Problem (VRP). We call it the memetic SOM. The approach is based on the standard SOM algorithm used as
a main operator in a population based search. This operator is combined with other derived operators specifically dedicated
for greedy insertion moves, a fitness evaluation and a selection operator. The main operators have a similar structure based
on the closest point findings and local moves performed in the plane. They can be interpreted as performing parallels and
massive insertions, simulating the behavior of agents which interact continuously, having localized and limited abilities.
This self-organizing process is intended to allow adaptation to noisy data as well as to confer robustness according to demand
fluctuation. Selection is intended to guide the population based search toward useful solution compromises. We show that the
approach performs better, with respect to solution quality and/or computation time, than other neural network applications
to the VRP presented in the literature. As well, it substantially reduces the gap to classical Operations Research heuristics,
specifically on the large VRP instances with time duration constraint. 相似文献
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Erika J. Obando Montoya Céline Mélin Nathalie Blanc Arnaud Lanoue Emilien Foureau Leslie Boudesocque Gildas Prie Andrew J. Simkin Joël Crèche Lucia Atehortùa Nathalie Giglioli‐Guivarc'h Marc Clastre Vincent Courdavault Nicolas Papon 《Yeast (Chichester, England)》2014,31(7):243-251
Candida guilliermondii (teleomorph Meyerozyma guilliermondii) is an ascomycetous species belonging to the fungal CTG clade. This yeast remains actively studied as a result of its moderate clinical importance and most of all for its potential uses in biotechnology. The aim of the present study was to establish a convenient transformation system for C. guilliermondii by developing both a methionine auxotroph recipient strain and a functional MET gene as selection marker. We first disrupted the MET2 and MET15 genes encoding homoserine‐O‐acetyltransferase and O‐acetylserine O‐acetylhomoserine sulphydrylase, respectively. The met2 mutant was shown to be a methionine auxotroph in contrast to met15 which was not. Interestingly, met2 and met15 mutants formed brown colonies when cultured on lead‐containing medium, contrary to the wild‐type strain, which develop as white colonies on this medium. The MET2 wild‐type allele was successfully used to transfer a yellow fluorescent protein (YFP) gene‐expressing vector into the met2 recipient strain. In addition, we showed that the loss of the MET2‐containing YFP‐expressing plasmid can be easily observed on lead‐containing medium. The MET2 wild‐type allele, flanked by two short repeated sequences, was then used to disrupt the LYS2 gene (encoding the α‐aminoadipate reductase) in the C. guilliermondii met2 recipient strain. The resulting lys2 mutants displayed, as expected, auxotrophy for lysine. Unfortunately, all our attempts to pop‐out the MET2 marker (following the recombination of the bordering repeat sequences) from a target lys2 locus were unsuccessful using white/brown colony colour screening. Nevertheless, this MET2 transformation/disruption system represents a new versatile genetic tool for C. guilliermondii. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
5.
Duhamel Cécilie Caballero Jacqueline Couvant Thierry Crépin Jérôme Gaslain Fabrice Guerre Catherine Le Hong-Thaï Wehbi Mickaël 《Oxidation of Metals》2017,88(3-4):447-457
Oxidation of Metals - The present work focuses on the intergranular oxidation of Alloy 600 and its weld material Alloy 82 after exposure in simulated primary water at 340–360 °C... 相似文献
6.
A new liquid chromatographic separation method was developed for the speciation of the four main arsenic compounds present in water. Arsenite (As(III)), dimethylarsinic acid (DMA), monomethylarsonic acid (MMA) and arsenate (As(V)) were separated on a recently introduced stationary phase: porous graphitic carbon (PGC). The separation was first obtained under formic acid gradient conditions, but an adsorption phenomenon of As(V) on PGC was observed. To overcome this problem, As(V) was backflushed, and an efficient separation of the four solutes was achieved within 10 min. Extremely low detection limits (ranging from 10 to 70 ng x L(-1)) were obtained by coupling LC with an ICPMS. The method was successfully applied to different spiked mineral waters and a naturally arsenic-containing freshwater. 相似文献
7.
Moya KL Salès N Hässig R Créminon C Grassi J Di Giamberardino L 《Microscopy research and technique》2000,50(1):58-65
We examined the localization of PrP(c) in normal brain using free-floating section immunohistochemistry and monclonal antibody 3F4. In the mature hamster and baboon brain, PrP(c) is localized to the neuropil with a synaptic distribution and the PrP(c) immunoreactivity is denser in regions known for ongoing plasticity. Cell bodies and major fiber tracts have little or no PrP(c) immunoreactivity. At the electron microscopic level, PrP(c) immunoreactivity decorates synaptic profiles, both pre- and postsynaptically. Results obtained with two additional antibodies, 3B5 and Pri-304, showed similar patterns of PrP(c) bands on Western blots, although Pri-304 was less sensitive. On sections through the adult hamster hippocampus, 3B5 and Pri-304 both stained the synaptic neuropil while cell bodies in the pyramidal and dentate granule cell layers were not immunoreactive. Pri-304 differentiated between synaptic layers in the hippocampus and closely resembled the pattern of staining obtained with 3F4. Preliminary results of developing brain showed that PrP(c) is initially localized along fiber tracts in the neonate brain. These results show that PrP(c) has a synaptic distribution in the adult brain and suggest that there are important changes in its distribution during brain development. These results also characterize two additional reagents for studies of PrP(c) localization. 相似文献
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A Ducastaing JL Azanza JM Robin J Raymond P Créac'h 《Canadian Metallurgical Quarterly》1976,58(7):771-782
Horse spleen cathepsin D (3.4.23.5.) was purified from crude extract by sodium chloride and ethanol precipitation, column chromatography fractionation on DEAE cellulose and CM Sephadex, re-chromatography on DEAE cellulose and gel filtration. The enzyme has been purified about 3.000 folds with a yield of 30 per cent. The purified enzyme seems to be homogeneous on Sephadex G100, one protein band is apparent on disc electrophoresis. Determined by dansylation the N-terminal amino acid is glycine. A molecular weight of 42,500 +/- 3,000 was obtained with Sephadex G100 gel filtration and light scattering measurements. Amino acid analysis and chemical determinations were performed: cathepsin D is a glycoprotein (2 or 3 osamine residues) including 344 amino acids and 4 disulfide bonds. Spectrophotometric data show that E1cm/1 mg/ml = 1.01 at lambda = 280 nm. ORD measurements indicate about 20 per cent of helicoidal content in the molecule. 相似文献
10.
Investigated kinematic reasoning, the equation of the dimensions of time, space, and velocity. 282 students, aged 8–17 yrs, made judgments about a spatial representation of duration (the mark made by a vibrator on moving tape) and about the velocity of the tape. Results indicate 2 types of errors: (a) errors concerning the inverse relationship between velocity and space (VE errors) and (b) errors regarding the differentiation of duration relative to kinematic frame (T errors). VE type errors were mostly observed at 8–9 yrs of age, while T errors were observed between 10 and 17 yrs of age. An important result was an observed "return" to the T type error (after a time lapse of 1 yr) with Ss producing correct response patterns. Errors are analyzed within the context of a theoretical model of kinematic reasoning. (29 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献