Further characterisation of protease inhibitors from pigeon pea (cajanus cajan (l) millsp) seeds

Cajanus trypsin inhibitor (CTI) and Cajanus trypsin-chymotrypsin inhibitor (CTCI) previously purified from cv TAT-10 were further characterised. The modification of the inhibitors revealed the presence of lysine at the trypsin reactive site in both CTI and CTCI. Modification of tyrosine at the reactive site of CTCI did not abolish chymotrypsin inhibition suggesting the presence of leucine or phenylalanine as reported in other chymotrypsin inhibitors. CTCI did not contain tryptophan. Dissociation constants for the inhibitors with bovine trypsin were in the region of 0.69 nmol (CTCI) and 0.029 nmol (CTI). Although the protease inhibitors lost their inhibitory activity on exposure to 2-mercaptoethanol they remained attached to the enzyme. The inhibitors were not very effective against the protease from Helicoverpa armigera which is a serious field pest of Cajanus. Dissociation constants for the inhibitors with the larval enzyme were in the region of 100 nmol.     

Photoluminescence,Judd–Ofelt analysis and photometric characterization of Eu<Superscript>3+</Superscript> activated Ca<Subscript>0.5</Subscript>Gd(WO<Subscript>4</Subscript>)<Subscript>2</Subscript> red phosphor

The red emitting Ca_0.5Gd(WO₄)₂:Eu³⁺ was synthesized by a solid-state reaction method. X-ray diffraction patterns were used to characterize crystal structure as well as phase purity. The results suggest that the as synthesized powder phosphor possess scheelite crystal structure with tetragonal symmetry along with the space group of I4₁/a. SEM studies reveal that the as synthesized sample show polyhedral morphology with particle size of 5.5 µm. Photoluminescence excitation spectrum depicts that a broad band (from 200 to 300 nm) centered at 242 nm is attributed to the ligand to metal charge transfer transition of WO₄ ^2? and three intense with sharp absorption bands (observed at 394, 464 and 535 nm) are designated as f–f electronic transitions of Eu³⁺. Photoluminescence emission studies indicate that, under 394 nm UV excitation, a hypersensitive red emission was observed at 617 nm due to the transition from upper ⁵D₀ level to the ⁷F₂ lower level of Eu³⁺ ion. The spectroscopic behaviour of the as synthesized phosphor Ca_0.5Gd(WO₄)₂:Eu³⁺ was determined using Judd–Ofelt theory. The CIE color coordinates, colour correlated temperature and luminous efficacies of radiation were estimated. The as obtained results indicating that the Ca_0.5Gd(WO₄)₂:Eu³⁺ red phosphor is most suitable for solid state lighting applications.     

Tribological properties of modified jojoba oil as probable base stoke of engine lubricant

Journal of Mechanical Science and Technology - This paper investigates the tribological characteristics of modified jojoba oil (MJO) as a base stoke for SAE20W40 mineral oil (LO). In addition, a...     

Development and Applications of Coarse-Grained Models for RNA

In contrast to proteins, much less attention has been focused on the development of computational models for describing RNA molecules, which are being recognized as playing key roles in many cellular functions. Current atomically detailed force fields are not accurate enough to capture the properties of even simple nucleic acid constructs. In this article, we review our efforts to develop coarse-grained (CG) models that capture the underlying physics for the particular length scale of interest. Two models are discussed. One of them is the three interaction site (TIS) model, in which each nucleotide is represented by three beads corresponding to sugar, phosphate, and base. The other is the self-organized polymer (SOP) model, in which each nucleotide is represented as a single interaction center. Applications of the TIS model to study the complexity of hairpin formation and the effects of crowding in a shifting equilibrium between two conformations in human telomerase pseudoknot are described. The work on crowding illustrates a direct link to the activity of telomerase. We use the SOP model to describe the response of the Tetrahymena ribozyme to force. The simulated unfolding pathways agree well with single molecule pulling experiments. We also review predictions for the unfolding pathways for the Azoarcus ribozyme. The success of the CG applications to describe dynamics in RNA gives hope that more complex processes involving RNA-protein interactions can be tackled using variants of the proposed models.     

Correlation between central receiver size and solar field using flat heliostats

In Central Receiver Systems (CRSs), thousands of heliostats track the sunrays and reflect beam radiation on to a receiver surface. The size of the reflected image and the extent of reflection from the heliostats are one of the important criteria that need to be taken into account while designing a receiver, since spillage losses may vary from 2 to 16% of the total losses. The present study aims to determine the size of an external cylindrical receiver, such that the rays reflected from all the heliostats in the field are intercepted. A dimensionless correlation with respect to tower height and receiver size (diameter and height) as a function of heliostat size and its position is discussed in the paper. This correlation could be used as a first-order approximation to estimate the receiver dimensions. When applied to the Ivanpah Solar Electricity Generating Station (ISEGS) plant, the correlation yields satisfactory estimation of receiver dimensions.     

Effect of microwave heat treating processing on frictional behaviour of aluminium alloy 2900 composites

The sliding wear behaviour of microwave processed, SiC_p and Al₂O_3p reinforced aluminium alloy 2900 and 2024 metal matrix composites prepared by powder metallurgy method was investigated in a pin on disc system. The objective is to determine the effects of novel alloying elements of AA 2900, ceramic addition and microwave aging process on the strength to tribological properties. This composite is evaluated to be an effective replacement for conventionally available AA 2024 composites in brake applications. Compared to conventional heat treating processes, microwave processing used for heat treating the samples is observed to be novel method in improving the strength–microstructural–tribological properties. AA 2900 with 6 wt-% Al₂O₃ exhibited good strength to microstructure relationship with excellent wear characteristics compared to AA 2024 composites which are governed by alloying elements in AA 2900. 2 H aged AA 2900 with 6 wt-% Al₂O₃ sample exhibited good frictional coefficient values with good density and strength characteristics. Hence, it is observed that alloying elements in AA 2900 and microwave processing have enhanced the strength to tribological behaviour where the property enhancement is achieved only through ceramic reinforcements.     

Assessment of flow induced vibration in a sodium-sodium heat exchanger

The 500 MWe Prototype Fast Breeder Reactor (PFBR) is under construction at Kalpakkam. It is a liquid metal sodium cooled pool type fast reactor with all primary components located inside a sodium pool. The heat produced due to fission in the core is transported by primary sodium to the secondary sodium in a sodium to sodium Intermediate Heat Exchanger (IHX), which in turn is transferred to water in the steam generator. PFBR IHX is a shell and tube type heat exchanger with primary sodium on shell side and secondary sodium in the tube side. Since IHX is one of the critical components placed inside the radioactive primary sodium, trouble-free operation of the IHX is very much essential for power plant availability. To validate the design and the adequacy of the support system provided for the IHX, flow induced vibration (FIV) experiments were carried out in a water test loop on a 60° sector model. This paper discusses the flow induced vibration measurements carried out in 60° sector model of IHX, the modeling criteria, the results and conclusion.     

Continuous anisotropic representation of coarse-grained potentials for proteins by spherical harmonics synthesis

A new method is presented for extracting statistical potentials dependent on the relative side chain and backbone orientations in proteins. Coarse-grained, anisotropic potentials are constructed for short-, medium-, and long-range interactions using the Boltzmann method and a database of non-homologous protein structures. The new orientation-dependent potentials are analyzed using a spherical harmonics decomposition method with real eigenfunctions. This method permits a more realistic, continuous angular representation of the coarse-grained potentials. Results of tests for discriminating the native protein conformations from large sets of decoy proteins, show that the new continuous distance- and orientation-dependent potentials present significantly improved performance. Novel graphical representations are developed and used to depict the orientational dependence of the interaction potentials. These new continuous anisotropic statistical potentials could be instrumental in developing new computational methods for structure prediction, threading and coarse-grained simulations.