DNA flow cytometric analysis in patients with operable non-small cell lung carcinoma

Fresh surgical specimens of tumors from 60 patients with previously untreated non-small cell lung carcinoma (NSCLC) who underwent radical surgery between January 1991 and October 1992 were investigated by means of flow-cytometry. The nuclear DNA measurement was carried out using a Facscan (Becton, Dickinson, USA). Analysis of the DNA content was performed in all 60 patients whilst cell cycle analysis was possible in 41 cases (68.3%). Forty-two of the 60 cases (70%) were aneuploid and 18 (30%) were diploid. The overall mean value of DNA index was 1.5. Diploid NSCLC were compared with aneuploid tumors: no significant differences in age distribution, sex ratio, histology and staging were found between the two groups (P > 0.05). An S-phase proportion of more than 10% was found in 30 out of 41 patients (73.2%). Early cancer deaths were reported in four patients (6.6%): the aneuploidy rate was very close in these patients (75%) and in the remaining surviving patients (69.6%). An S-phase proportion of more than 10% was found in 100% of early cancer deaths and in 70.2% of the remaining cases; such a difference seems of some importance although it was not statistically significant (P = 0.071). In conclusion, flow-cytometry studies seem to be a useful tool in the understanding of the biological behavior of patients with NSCLC. In the present prospective report there were no significant correlations between DNA measurements and clinical outcome, however, these results suggest that a high S-phase proportion should be seen as a possible prognostic indicator.(ABSTRACT TRUNCATED AT 250 WORDS)     

Effects of benazepril on stress testing blood pressure in essential hypertension

The effects of different doses of the angiotensin-converting enzyme inhibitor benazepril on cardiovascular response to a set of standardized laboratory tasks were analyzed. Eighteen patients (15 men and 3 women) with mild-to-moderate essential hypertension were randomly allocated to receive 10 or 20 mg of benazepril, or placebo, each administered once daily for 2 weeks, according to a double-blind, 3-period design. At the end of each treatment period, patients were examined at resting baseline and while performing mental arithmetic, handgrip and cycle ergometry tests. In comparison with placebo, the average reductions in resting systolic blood pressure (BP) were 8.7 mm Hg (95% confidence intervals [CI] -15.2 to -2.1) with 10 mg of benazepril, and 7.8 mm Hg (95% CI -14.4 to -1.3) with 20 mg; the corresponding reductions in resting diastolic BP were 5.1 mm Hg (95% CI -8.7 to -1.4) and 6.8 mm Hg (95% CI -10.4 to -3.1) (all p < 0.05). During mental arithmetic, the reductions in systolic BP were 10.4 mm Hg (95% CI -17.4 to -3.4) with 10 mg of benazepril, and 13.8 mm Hg (95% CI -20.8 to -6.8) with 20 mg; diastolic BP was reduced by 4.5 mm Hg (95% CI -8.5 to -0.5) and 8.3 mm Hg (95% CI -13.2 to -4.3), respectively (all p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)     

A dynamic programming approach to sequential pattern recognition

This paper presents the dynamic programming approach to the design of optimal pattern recognition systems when the costs of feature measurements describing the pattern samples are of considerable importance. A multistage or sequential pattern classifier which requires, on the average, a substantially smaller number of feature measurements than that required by an equally reliable nonsequential classifier is defined and constructed through the method of recursive optimization. Two methods of reducing the dimensionality in computation are presented for the cases where the observed feature measurements are 1) statistically independent, and 2) Markov dependent. Both models, in general, provide a ready solution to the optimal sequential classification problem. A generalization in the design of optimal classifiers capable of selecting a best sequence of feature measurements is also discussed. Computer simulated experiments in character recognition are shown to illustrate the feasibility of this approach.     

Design of the winding–bushing interconnections in large power transformers

The paper presents the improvement in design of the conductor connecting the windings and bushings in oil power transformers. This is a sensitive part of large transformers and there is a need to optimise its manufacturing time and costs. The thermal problem of the heating of this conductor with increased insulation thickness on a part of it is treated using a non-linear two-dimensional thermal model. The experiments performed made determining of some problematic parameters (heat resistance of oil-paper insulation and the paper to oil convection heat transfer coefficient) of the thermal model feasible. The results obtained are of practical interest in the design practice of interconnections, but also affect the important parameters of heat transfer by devices with oil immersed paper insulation.     

An optimum finite sequential procedure for feature selection and pattern classification

In this paper a quite general formulation of sequential pattern recognition processes is presented. Within the framework of this formulation, a procedure is obtained for the simultaneous optimization of the stopping rule and the stage-by-stage ordering of features as the process proceeds. This optimization procedure is based on dynamic programming and uses as an index of performance the expected cost of the process, including both the cost of feature measurement and the cost of classification errors. A simple example illustrates the important computational aspects of the procedure and indicates the form of the solution.     

A system for classifying mechanical injuries of the eye (globe). The Ocular Trauma Classification Group

PURPOSE: To develop a classification system for mechanical injuries of the eye. METHODS: The Ocular Trauma Classification Group, a committee of 13 ophthalmologists from seven separate institutions, was organized to discuss the standardization of ocular trauma classification. To develop the classification system, the group reviewed trauma classification systems in ophthalmology and general medicine and, in detail, reports on the characteristics and outcomes of eye trauma, then established a classification system based on standard terminology and features of eye injuries at initial examination that have demonstrated prognostic significance. RESULTS: This system classifies both open-globe and closed-globe injuries according to four separate variables: type of injury, based on the mechanism of injury; grade of injury, defined by visual acuity in the injured eye at initial examination; pupil, defined as the presence or absence of a relative afferent pupillary defect in the injured eye; and zone of injury, based on the anteroposterior extent of the injury. This system is designed to be used by ophthalmologists and nonophthalmologists who care for patients or conduct research on ocular injuries. An ocular injury is classified during the initial examination or at the time of the primary surgical intervention and does not require extraordinary testing. CONCLUSIONS: This classification system will categorize ocular injuries at the time of initial examination. It is designed to promote the use of standard terminology and assessment, with applications to clinical management and research stud ies regarding eye injuries.     

Terrain height measurement accuracy of interferometric syntheticaperture radars

Presents a simple expression for the accuracy with which an interferometer synthetic-aperture radar (IFSAR) can measure terrain elevation. The expression, derived analytically and confirmed by Monte Carlo simulation, accounts for thermal noise, resolution cell size, terrain slope and roughness, volume scattering above the terrain, radar-terrain geometry, interferometer baseline, and radar frequency. This paper takes a “glint” approach to assessing the impact of scatterers distributed in angle. The results show that there is a residual uncertainty in the height of a pixel due to its angular extent, even when the signal-to-noise ratio is very large. The analysis identifies an optimum range resolution for minimizing the height uncertainty for a particular terrain slope     

A wet process technology applied to jojoba seed to obtain oil and detoxified protein meal

An industrial wet process to obtain oil and meal from jo-joba was set up. The process sequence consists of breaking the seeds, homogenizing with water of suitable pH and temperature, and centrifuging to accomplish separation into oil, process water and wet meal. Oil is obtained with a yield of 70–75% and requires no supplementary refining treatment for the industrial purposes for which it is destined. The meal obtained is devoid of the toxic components simmondsin and simmondsin-2′ ferulate, and the protein content may be considered unchanged. The procedure contemplates a drying treatment for the meal with a view to using it as animal feed. This system is simple, economical and flexible in use.     

Racial differences in nitric oxide-mediated vasodilator response to mental stress in the forearm circulation

An abnormal hemodynamic response to stressful stimuli has been proposed as a mechanism involved in the higher prevalence of hypertension in blacks. Given the important role of nitric oxide (NO) in the regulation of cardiovascular homeostasis, we investigated the possibility of racial differences in vascular NO activity during mental stress. To test this hypothesis, we compared the forearm blood flow (FBF) response to mental stress in 14 white and 12 black healthy subjects during intra-arterial infusion of either saline or NO synthesis inhibitor N(G)-monomethyl-L-arginine (L-NMMA; 4 micromol/min). We also examined vascular responses of the two groups to intra-arterial infusion of sodium nitroprusside (0.8 to 3.2 microg/min), an exogenous NO donor. During saline infusion, the increase in FBF from baseline induced by mental stress was significantly higher in whites than in blacks (109+/-20% versus 58+/-8%; P=0.03). L-NMMA significantly reduced stress-induced increase in FBF in whites (from 109+/-20% to 54+/-11%; P=0.004) but not in blacks (from 58+/-8% to 42+/-10%; P=0.24); thus, the vasodilator effect of stress testing during L-NMMA was similar in whites and blacks (54+/-11% versus 42+/-10%; P=0.44). The vasodilator response to sodium nitroprusside was also lower in blacks than in whites (maximum flow, 6.9+/-2 versus 11.6+/-3.5 mL x min(-1) x dL(-1); P=0.001) and was not significantly modified by L-NMMA in either group. Our findings indicate that blacks have a reduced NO-dependent vasodilator activity during mental stress. This difference seems related to reduced sensitivity of smooth muscle to the vasodilator effect of NO and may play some role in the increased prevalence of hypertension and its complications in blacks.