Family-Responsive Interventions, Perceived Organizational and Supervisor Support, Work-Family Conflict, and Psychological Strain.

This study examined organizational family-responsive policies, perceptions of the organization as family supportive, and supervisor support as issues that may be salient to the experience of conflict between paid employment (work) and family roles. Data were collected from 355 managerial personnel in New Zealand. Although work-family conflict and psychological strain were strongly linked, the availability of organizational policies had no significant association with levels of conflict or strain, whereas policy usage was related only to work-to-family interference and not to family-to-work interference. On the other hand, perceptions of the organization as family supportive and supervisor support for work-family balance displayed significant relationships with key variables, highlighting the importance of these variables for interventions designed to ameliorate the negative impact of work-family conflict on managerial well-being. Implications for the effective implementation of family-responsive interventions are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Quantifying blood flow and wall shear stresses in the outflow tract of chick embryonic hearts

Wall shear stresses (WSS) exerted by blood flow on cardiac tissues modulate growth and development of the heart. To study the role of hemodynamic conditions on cardiac morphogenesis, here, we present a methodology that combines imaging and finite element modeling to quantify the in vivo blood flow dynamics and WSS in the cardiac outflow tract (OFT) of early chicken embryos (day 3 out of 21-day incubation period). We found a distinct blood flow field and heterogeneous distribution of WSS in the chicken embryonic heart OFT, which have physiological implications for OFT morphogenesis.     

Pulse-burst laser-based 10kHz Thomson scattering measurements

Thomson scattering(TS),as a popular and reliable diagnostic technique,has successfully measured electron temperatures and electron number densities of plasmas for many years.However,conventional TS techniques using Nd:YAG lasers operate only at tens of hertz.Here,we present the development of a high-repetition-rate TS instrument based on a high-speed,pulse-burst laser system to greatly increase the temporal resolution of measurements.Successful instrument prototype testing was carried out by collecting TS light from laboratory helium and argon plasmas at 10 kHz.Calibration of the instrument detection sensitivity using nitrogen/oxygen rotational Raman scattering signal is also presented.Quantitative electron number densities and electron temperatures of the plasma were acquired at 10 kHz,for stable plasma discharges as,respectively,～0.9 eV and ～5.37×10~(21)m~(-3) for the argon plasma,and ～1eV and ～6.5×1021 m~(-3) for the helium plasma.     

Development of colorimetric analytical methods to monitor quaternary amine grafted surfaces

Quick and simple colorimetric analytical techniques were developed to monitor functionalization of surfaces with quaternary amine bearing polymers as complementary qualitative tests to the traditionally used antimicrobial test protocols. First, an established antimicrobial quaternary ammonium salt (QAS) was chosen as a probe compound and an acrylol functional group was introduced (acrylol‐QAS) to facilitate its polymerization with acrylic monomers. Surface graft polymerization was carried out with methyl methacrylate (MMA) and acrylol‐QAS on cotton fabric surface. The success of graft polymerization, durability after repetitive aqueous extraction, and the effect of crosslinking agents on the durability and extent of polymerization were tested with a fluorescein spot and a bromophenol solution test. The results of these colorimetric analytical methods were in perfect agreement with those of antimicrobial tests, XPS, and SEM analysis. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010     

The dual phases of the response to neonatal exposure to a VH family-restricted staphylococcal B cell superantigen

In vitro studies of several naturally occurring proteins have characterized VH family-specific B lymphocyte binding and stimulatory properties that appear analogous to those of T cell superantigens. To examine the in vivo consequences of exposure to a putative B cell superantigen, we treated neonatal BALB/c mice with a form of staphylococcal protein A (MS) devoid of Fcgamma binding activity, which retains the clan VHIII Fab binding specificity. In naive adults, about 5% of peripheral B cells and >13% of splenic IgM-secreting cells display MS binding activity, in association with high IgM and low IgG circulating anti-MS Ab titers. Neonatal exposure to MS elicited two distinct temporal phases of immune responsiveness. The early phase, representing the first approximately 5 wk of life, was associated with MS-specific B cell and T cell tolerance. Microfluorometric assays revealed that exposure caused a dramatic MS-specific B cell clonal loss in bone marrow and spleen, but levels normalized by about 3 wk of life. The late phase (>6 wk of age) was associated with spontaneous priming for MS-specific T cell responses and production of MS-specific IgG1 Abs despite long term persistently depressed in vivo and in vitro MS-specific IgM responses. In vivo challenge during the late phase induced high frequencies of MS-specific IgG-secreting cells, indicating recruitment of highly focused Ab responses that were predominantly encoded by rearrangements of the S107 family, a member of the VHIII clan. These studies document the immunodominance of the VH-restricted Fab binding site on staphylococcal protein A and demonstrate the diverse effects of a B cell superantigen on the emerging peripheral B cell compartment.     

A central region of Ku80 mediates interaction with Ku70 in vivo

Ku, the DNA binding component of DNA-dependent protein kinase (DNA-PK), is a heterodimer composed of 70 and 86 kDa subunits, known as Ku70 and Ku80 respectively . Defects in DNA-PK subunits have been shown to result in a reduced capacity to repair DNA double-strand breaks. Assembly of the Ku heterodimer is required to obtain DNA end binding activity and association of the DNA-PK catalytic subunit. The regions of the Ku subunits responsible for heterodimerization have not been clearly defined in vivo . A previous study has suggested that the C-terminus of Ku80 is required for interaction with Ku70. Here we examine Ku subunit interaction using N- and C-terminal Ku80 deletions in a GAL4-based two-hybrid system and an independent mammalian in vivo system. Our two-hybrid study suggests that the central region of Ku80, not its C-terminus, is capable of mediating interaction with Ku70. To determine if this region mediates interaction with Ku70 in mammalian cells we transfected xrs-6 cells, which lack endogenous Ku80, with epitope-tagged Ku80 deletions carrying a nuclear localization signal. Immunoprecipitation from transfected cell extracts revealed that the central domain identified by the GAL4 two-hybrid studies stabilizes and co-immunoprecipitates with endogenous xrs-6 Ku70. The central interaction domain maps to the internally deleted regions of Ku80 in the mutant cell lines XR-V9B and XR-V15B. These findings indicate that the internally deleted Ku80 mutations carried in these cell lines are incapable of heterodimerization with Ku70.     

Evaluation of current techniques for isolation of chars as natural adsorbents

Chars in soils or sediments may potentially influence the soil/sediment sorption behavior. Current techniques for the isolation of black carbon including chars rely often on acid demineralization, base extraction, and chemical oxidation to remove salts and minerals, humic acid, and refractory kerogen, respectively. Little is known about the potential effects of these chemical processes on the char surface and adsorptive properties. This study examined the effects of acid demineralization, base extraction, and acidic Cr2O7 2- oxidation on the surface areas, surface acidity, and benzene adsorption characteristics of laboratory-produced pinewood and wheat-residue chars, pure or mixed with soils, and a commercial activated carbon. Demineralization resulted in a small reduction in the char surface area, whereas base extraction showed no obvious effect. Neither demineralization nor base extraction caused an appreciable variation in benzene adsorption and presumably the char surface properties. By contrast, the Cr2O7 2- oxidation caused a >31% reduction in char surface area. The Boehm titration, supplemented by FTIR spectra, indicated that the surface acidity of oxidized chars increased by a factor between 2.3 and 12 compared to non-oxidized chars. Benzene adsorption with the oxidized chars was lower than that with the non-oxidized chars by a factor of >8.9; both the decrease in char surface area and the increase in char surface acidity contributed to the reduction in char adsorptive power. Although the Cr2O7 2-oxidation effectively removes resistant kerogen, it is not well suited for the isolation of chars as contaminant adsorbents because of its destructive nature. Alternative nondestructive techniques that preserve the char surface properties and effectively remove kerogen must be sought.     

Variation in in vitro alpha-amylase and protease activity is related to the virulence of Aspergillus flavus isolates

Variation in the ability of Aspergillus flavus isolates to spread between cotton boll locules was previously shown to be at least partially related to variation in the production of a specific polygalacturonase (pectinase P2C). To determine if non-pectolytic hydrolase differences between low- and high-virulence isolates exist and, thus, could also potentially contribute to virulence differences, the present investigation was undertaken. Two A. flavus isolates, AF12 with low virulence and lacking pectinase P2C and AF13 with high virulence and producing pectinase P2C, were compared for production of nonpectolytic hydrolases after growth in 10% potato dextrose broth. Activity of amylases, cellulases, xylanases, and proteases was quantified using the radial diffusion/cup plate technique followed by differential staining. Culture filtrates also were subjected to native polyacrylamide gel electrophoresis. Both isolates produced amylases, proteases, and xylanases, whereas cellulases were not detected for either. AF13 produced more amylase than AF12, and this difference was supported by amylase isoform differences between isolates on native polyacrylamide gel electrophoresis gels. AF13 also produced more protease than AF12; however, isoform differences between isolates were inconclusive. These variations in other hydrolytic activities (besides pectinases) may contribute to virulence differences in cotton bolls between AF12 and AF13.     

Cognitive ability predicts objectively measured counterproductive work behaviors.

Over the past 2 decades, increasing attention has been directed at the relationship between individual differences and counterproductive work behaviors (CWB). However, most of this research has focused on personality variables as potential predictors of CWB; surprisingly little research has investigated the link between counterproductivity and cognitive ability. This study presents the first focal investigation of the cognitive ability-CWB relationship. The authors measured organizational and interpersonal CWB using organizational records of formally recorded incidents (e.g., destruction of property, physical violence). In a predictive study, for a large sample of law enforcement job applicants, a standardized psychometric test of cognitive ability predicted CWB, whereas educational attainment did not. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Distributing working memory resources during problem solving.

This study examines how problem solvers distribute working memory demands over internal and external resources. Participants recorded notes while performing an arithmetic task. They recorded a majority of intermediate results and labeled many of those results (e.g., "C?=?10"). When more effort was required to take notes, participants recorded fewer results. Participants with a consistent goal structure recorded fewer results and with practice labeled fewer recorded results than those with varied goal structures. When notes were displayed in a consistent spatial arrangement participants labeled fewer recorded results than when notes appeared in varied locations. These findings indicate that individuals use explicit and implicit strategies for indexing intermediate results. The data support the view that individuals flexibly distribute working memory over internal and external resources in response to situational cost-benefit considerations. (PsycINFO Database Record (c) 2010 APA, all rights reserved)