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2.
Sensory activity mediates regulation of tyrosine hydroxylase (TH), the first enzyme in the dopamine biosynthetic pathway, in the rodent olfactory bulb. The current studies established for the first time primary cultures of neonatal mouse olfactory bulb expressing TH and tested whether L-type calcium channels mediate the activity-dependent regulation of the dopamine phenotype. After 1 d in vitro (DIV), a small population of TH-immunostained neurons that lacked extensive processes could be demonstrated. After an additional 2 DIV in serum-free medium, the number of TH neurons had doubled, and they exhibited long interdigitating processes. Membrane depolarization for 48 hr with 50 mM KCl produced a further 2.4-fold increase in the number of TH-immunoreactive neurons compared with control cultures. Increased TH neuron number required at least 36 hr of exposure to KCl. Forskolin, which increases intracellular cAMP levels, induced a 1.5- to 1.6-fold increase in the number of TH-immunostained neurons. Combined treatment with KCl and forskolin was not additive. Nifedipine, an L-type calcium channel blocker, completely prevented the depolarization-mediated increase in TH expression but did not block the response to forskolin. Treatment with Bay K8644, an L-type calcium channel agonist, also significantly increased the number of TH-expressing neurons. Depolarization also induced alterations in neuritic outgrowth, resulting in a stellate versus an elongate morphology that, in contrast, was not prevented by nifedipine. These results are the first demonstration that in vitro, as in vivo, depolarization increases TH expression in olfactory bulb and that L-type calcium channels mediate this activity-dependent regulation of the dopamine phenotype. 相似文献
3.
Structural engineers use design codes formulated to consider uncertainty for both reinforced concrete and structural steel
design. For a simple one-bay structural steel frame, we survey typical uncertainties and compute an interval solution for
displacements and forces. The naive solutions have large over-estimations, so we explore the Mullen-Muhanna assembly strategy,
scaling, and constraint propagation to achieve tight enclosures of the true ranges for displacements and forces in a fraction
of the CPU time typically used for simulations. That we compute tight enclosures, even for large parameter uncertainties used
in practice, suggests the promise of interval methods for much larger structures. 相似文献
4.
Norris Fran H.; Murphy Arthur D.; Baker Charlene K.; Perilla Julia L.; Rodriguez Francisco Gutiérrez; Rodriguez José de Jesús Gutiérrez 《Canadian Metallurgical Quarterly》2003,112(4):646
Prevalence rates of trauma and posttraumatic stress disorder (PTSD) were estimated from a probability sample of 2,509 adults from 4 cities in Mexico. PTSD was assessed according to Diagnostic and Statistical Manual of Mental Disorders (American Psychiatric Association, 1994) criteria using the Composite International Diagnostic Interview (CIDI; WHO, 1997). Lifetime prevalence of exposure and PTSD were 76% and 11.2%, respectively. Risk for PTSD was highest in Oaxaca (the poorest city), persons of lower socioeconomic status, and women. Conditional risk for PTSD was highest following sexual violence, but nonsexual violence and traumatic bereavement had greater overall impact because of their frequency. Of lifetime cases, 62% became chronic; only 42% received medical or professional care. The research demonstrates the importance of expanding the epidemiologic research base on trauma to include developing countries around the world. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
5.
The short contact time dissolution of vitrinite and inertinite concentrates of an Australian bituminous coal was studied under isothermal conditions with tetralin as solvent. The reactivity of the two maceral types and the yields and nature of the products were determined for a range of reaction temperatures (400, 420 and 440 °C) and reaction times (2–20 min). The dissolution of vitrinite proceeded at a significantly greater rate than for inertinite and was associated with greater selectivity to soluble non-gaseous products. At very short reaction times (2 min), the vitrinite underwent solubilizing reactions to yield products of higher molecular weight and greater oxygen functionality than was the case for inertinite, for which changes in the chemical nature of the products were negligible over the range of conditions studied. At longer reaction times (5 min), the composition of the vitrinite-derived products was similar to that of the inertinite-derived products. 相似文献
6.
M. A. Amer D. B. Kupranycz B. E. Baker 《Journal of the American Oil Chemists' Society》1985,62(11):1551-1557
Both summer and winter butterfats were fractionated using a laboratory procedure which was designed to simulate a commercial
fractionation process. The process is based on a slow, controlled cooling of the melted fat, a short stabilization time at
the fractionation temperature, and separation of the crystals from the liquid oil by vacuum filtration using a stainless steel
perforated disc. Fractionation temperatures of 29, 26, 23 and 19 C for winter butterfat and 29 and 19 C for summer butterfat
were used to obtain solid and liquid fractions at each temperature. Three replications at each temperature showed good reproducibility
of results. The fractions were characterized by their fatty acid and triglyceride compositions, melting and crystallization
behavior, iodine value, peroxide value and melting point.
Presented at the AOCS meeting in Philadelphia in May 1985. 相似文献
7.
C. B. Winkelmann E. Collin Yu. M. Bunkov H. Godfrin 《Journal of Low Temperature Physics》2004,135(1-2):3-14
We report systematic measurements of the response of a Vibrating Wire Resonator (VWR) in normal and superfluid liquid 3He. Special attention has been paid to the hydrodynamic regime of the superfluid B-phase, where the response parameters of the VWR do not follow a simple law. We show that a simple interpolation between the region where first order slip-corrections can be applied and the ballistic regime is insufficient. Measuring an empirical effective viscosity, we propose a temperature calibration method which allows the use of VWRs as a secondary thermometer at intermediate and high pressures in the temperature range 0.2 T c < T < 50 mK. 相似文献
8.
The performance of netted radar is evaluated in terms of a netted version of the radar equation and the ambiguity function. A software simulation has been developed to allow assessment of netted radar sensitivity and ambiguity properties in both two-and three-dimensional space. Simulation results demonstrate that coherent netted radar sensitivity depends not only on the radar parameters, but also on the numbers, locations and specification of transmit and receive nodes. The netted radar ambiguity function is affected by a combination of many factors. In addition to those that determine the form of the monostatic ambiguity function, the locations of transmit and receive nodes as well as the position and velocity of the target all are shown to play an important role in evaluating system performance. As a result of these dependencies, it is shown that there is severe degradation of range and Doppler resolution when a target crosses sufficiently close to a transmit-receive baseline. 相似文献
9.
J. K. Richard Weber† Collin D. Anderson Dennis R. Merkley Paul C. Nordine† 《Journal of the American Ceramic Society》1995,78(3):577-582
Solidification of aluminum oxide from undercooled melts was investigated in containerless experiments. Specimens were levitated in a gas jet, stabilized with an acoustic positioning device, and melted with cw CO2 laser beams. Cooling curves were obtained by optical pyrometry when the laser intensity was reduced. The materials examined were high-purity Verneuil sapphire, 99.5% polycrystalline alumina, and oxide materials recovered from the effluent of an aluminum-fueled rocket motor. The degree of undercooling, the apparent temperature behavior during the thermal arrest on solidification, and the structure of the materials formed were different in argon and oxygen atmospheres. Undercooling of the sapphire and alumina materials was 360 ± 10 K in an oxygen atmosphere and approximately 450 K in argon. Melting and solidification of high-purity sapphire resulted in a dendritic and porous polycrystalline material in oxygen. Dense, larger crystals were obtained in argon. Products formed from 99.5% alumina were discolored and the cores were white, indicating impurity segregation effects. More reproducible behavior was observed for the sapphire and 99.5% alumina than for the tungstencontaminated rocket motor effluent materials. 相似文献
10.