Comparison of three monoclonal antibody pools for the detection of respiratory viral antigen in respiratory secretions

The aim of this study was to compare the sensitivities of commercial monoclonal antibody pools to be used as an initial rapid screen for detection of viral antigens in respiratory secretions. The availability of commercial monoclonal antibodies has dramatically improved the detection of viruses by immunofluorescence techniques in exfoliated cells obtained from respiratory secretions. Several companies have recently introduced monoclonal antibody pools to detect the presence of respiratory viruses in a single preparation. Ninety-four stored slide preparations that had previously been examined by individual monoclonal antibodies were tested using three commercial monoclonal antibody pools produced by Sanofi (UK), Dako (UK), and Quadratech (UK). These monoclonal antibody pools had a sensitivity of 79.6%, 90.9%, and 100%, respectively, when compared with the original results. The overall intensity of immunofluorescence was also examined.     

The influence of viewing distance on subjective impairment assessment

In this paper, the influence of viewing distance on subjective assessment of the impairment in video sequences is investigated. Subjective tests using the double-stimulus impairment scale variant II (DSIS II) method have been conducted at viewing distances of 5H (where H stands for the screen height) and 3H, respectively. Several statistical measures have been used to analyze the influence, including correlations and ANOVA (analysis of variance) tests. The results reveal that there is a very high correlation between the subjective scores, the variances are similar under the two viewing distances, the means of subjective data at these two viewing distances are the same, and there is no interaction between the viewing distance and the other two factors, i.e., the codec system and the source sequence. Throughout the tests, there is no evidence that a closer viewing distance such as 3H will vary the subjective test result statistically significantly.     

Cryptosporidium Log-inactivation with Ozone Using Effluent CT10, Geometric Mean CT10, Extended Integrated CT10 and Extended CSTR Calculations

The draft Long-Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR) contains Cryptosporidium log-inactivation CT tables (ozone-in-water concentration [residual], “C” times contact time, T). Depending on water temperature, Cryptosporidium CT values that are listed are 15 to 25 times greater than CT values for equivalent Giardia log-inactivation credit. The elevated operating dose required for Cryptosporidium log-inactivation credit has the potential to increase disinfection by-product (DBP) formation (e.g., bromate). Calculating CT value accurately will minimize ozone dose, which will decrease operating cost and lower DBP formation, and at the same time maintain disinfection protection through implementation of scientifically based safety factors. Various methods are available for calculating CT value. The method chosen depends largely on the available information concerning ozone residual characteristics and hydrodynamic features of the ozone contactor, plus local regulatory requirements. Four methods are discussed in this paper. Each method can be used to calculate Giardia, virus, and Cryptosporidium log-inactivation credit.     

Nanostructured polymer blends: Synthesis and structure

Nanostructured polymer blends prepared via anionic ring opening polymerizations of cyclic monomers in the presence of a pre-made polymer melt exhibit a number of special properties over traditional polymer blends and homopolymers. Here, we report on a simple and versatile method of in situ polymerization of macrocyclic carbonates in the presence of a maleic anhydride polypropylene (mPP) matrix and a surface-active compatibilizer (i.e. PC grafted onto a mPP backbone generated in situ) to yield a micro- and nanostructured polymer blends consisting of a polycarbonate (PC) minor phase, and a polypropylene (PP) major phase. By varying the processing conditions and concentration of the macrocyclic carbonate it was possible to reduce the size of the PC dispersions to an average minor diameter of 150 nm. NMR and TEM characterizations indicate that the PC dispersions do not influence crystal content in the PP phase. Overall, the results point to a simple strategy and versatile route to new polymeric materials with enhanced benefits.     

Unicode Steganographic Exploits: Maintaining Enterprise Border Security

Unicode is rapidly becoming the preferred means for representing symbols used in creating multimedia content, especially for information that's presented in multiple languages. This article discusses a unicode vulnerability that makes such content susceptible to being used for creation of covert channel communications. We also developed a solution architecture, the unified secure message augmentation (USMA) service. The USMA service incorporates rules (in an XML vocabulary) that we can apply to unicode transmissions that will detect an attempt to transmit a potential exploit, alert network managers to the presence of the unicode anomaly, and take action to mitigate the exploit.     

Designing the world we live in

Engineering designers, who decide the physical shape and technical features of the built world, tend to forget that their predecessors have been making design decisions for hundreds of years. This paper gives several historical examples of design experience which can supply useful insights today, because the essential nature of engineering has not changed.Adapted from: Eugene S. Ferguson,Engineering and the Mind's Eye. Cambridge, Massachusetts: MIT Press, 1992     

Measurement and prediction of pressure drop in two-phase flow

Experimental data for air–water two-phase co-current flow in two different pipe diameters were used to test the prediction of pressure drop by a number of existing theories and correlations. Several models are shown to be useful for prediction, particularly with the stratified regimes which have proved difficult to handle in the past. The model suggested by Olujic proved to be of particular value.     

Effect of surface boundary on neuronal magnetic stimulation

The effect of the surface boundary between free space and a conducting medium on the excitation properties of neurons by magnetic fields are analyzed. The electric field and the spatial derivative of the induced field generated by a coil mounted both parallel and perpendicular to the surface of a semi-infinite conducting medium were calculated using the method of images. An imaginary axon is located in the same relative position from the coil in both configurations and the excitation properties are compared. The calculations are expressed in terms of the activating function for the electrical stimulation of axons. The calculations indicate that the activating function for magnetic stimulation is biphasic as opposed to triphasic for electrical stimulation. The large spatial extent of the magnetically induced electric field compared to the electric field generated by point source electrode suggests a different mode of excitation for neuronal structures in the CNS. The field distribution have been verified experimentally and are important for the understanding of the mechanisms of magnetic stimulation of neural tissue.