About the origins of residual stresses in <Emphasis Type="Italic">in situ</Emphasis> consolidated thermoplastic composite rings

In a context of cost reduction, in situ filament winding of thermoplastic matrix composites becomes an appealing process. As residual stresses could considerably affect the produced part, models were proposed to predict process-induced residual stresses. After developing a validated thermal model of the process, mainly three different aspects are here addressed: the continuous bonding occurring during the process, the effect of the processed layer on the structure, and the effect of the curvature of the mandrel. While stresses coming from the continuous bonding appeared to be negligible, consequent levels of stresses can be achieved due to an iterative compression of the structure by the tow (supposed to be under tension). The mandrel properties and the tow tension profile followed during winding are essential parameters that might induce several different stress states. A comparison between measured and computed end-to-end openings of split rings illustrates the accuracy of the proposed models.     

p34cdc2 expression and meiotic competence in growing goat oocytes

Proper control of environmental factors can be crucial to the identification of genes that influence susceptibility to a complex trait, especially for a trait such as lung cancer, for which the environmental factor (smoking) accounts for a significant etiologic fraction of the disease. An earlier segregation analysis of 337 Louisiana families, which incorporated direct measure of tobacco consumption, provided evidence for autosomal codominant inheritance of a major gene that influenced age at onset of lung cancer. Subsequent analyses were performed in which the families were stratified into two subsets based on birth cohort of the proband; results suggested the presence of heterogeneity that were postulated to reflect the influence of cohort trends in tobacco consumption. To evaluate this hypothesis further, we simulated a population of three-generation pedigrees in which an autosomal dominant mode of susceptibility to lung cancer was transmitted, but tobacco use varied across generations corresponding to published trends in smoking. A total of 200,000 individuals in families of various sizes, ages, and cigarette smoking habits were simulated from 1900 to 1980. From this population, 324 families (2,405 individuals) with 380 cases of lung cancer were ascertained through 328 lung cancer probands. Complex segregation analysis was performed using the REGTL program of S.A.G.E. in which pack-years of tobacco exposure were incorporated directly into the likelihood calculations. Although the no major gene, environmental, and Mendelian recessive hypotheses were rejected, both dominant and codominant transmission provided a good fit to the data. Thus in a population of simulated families with autosomal dominant susceptibility to lung cancer, intergenerational differences in tobacco consumption led to the detection of autosomal codominant transmission as an acceptable hypothesis. These results underscore the potential danger of segregation analysis of complex traits in which exposure to known environmental influences may differ across generations.     

Reduction of restenosis after angioplasty in an atheromatous rabbit model by suicide gene therapy

BACKGROUND: Gene delivery of the thymidine kinase (tk) gene combined with ganciclovir (GCV) limits intimal hyperplasia after abrasion of normal arteries. However, the low efficiency of adenoviral-mediated gene transfer to atherosclerotic arteries has raised concerns about the applicability of this strategy to the prevention of restenosis. METHODS AND RESULTS: A replication-defective adenoviral vector expressing tk (Ad-RSVtk) demonstrated selective toxicity toward GCV-treated arterial smooth muscle cells, with oligonucleolytic cleavage suggesting apoptosis. In vivo, after demonstration of tk expression after Ad-RSVtk delivery, the combination of Ad-RSVtk followed by GCV was tested in a rabbit model of angioplasty of atheromatous iliac arteries. Angioplasty (8 atm, 20 minutes) was performed by use of a hydrogel balloon coated with Ad-RSVtk (4x10(9) plaque forming units). GCV was infused (25 mg.kg(-1) I.V. BID) from days 2 through 7 after angioplasty in 8 of 12 rabbits. Four weeks later, morphometric analysis demonstrated a reduced intima-to-media ratio in the group receiving combination therapy compared with Ad-RSVtk alone (3.0+/-1.2 versus 5.2+/-0.5, P<.018). GCV per se had no effect on intimal hyperplasia after arterial injury. CONCLUSIONS: In vitro, Ad-RSVtk demonstrates selective toxicity toward GCV-treated arterial smooth muscle cells involving apoptosis. In vivo, GCV conditions reduction of neointimal formation after percutaneous delivery of Ad-RSVtk during angioplasty of atheromatous arteries.     

Freeze-fracture electron microscopic analysis of solutions of biological molecules

Freeze-fracture electron microscopy was used to study the morphology of proteins in solution. The sizes of the particles appearing on the fractured surfaces, replicated with tungsten-tantalum, were measured in a direction perpendicular to the shadowing angle. The distributions of the measured particle sizes could be correlated with the known shape and dimensions for each protein. It is concluded that freeze-fracture electron microscopy is a useful technique to study the morphology of biological molecules in solution, particularly hydrophobic proteins which may be difficult to study by other microscopic techniques.     

Correction of aerosol effects on multi-temporal images acquired with constant viewing angles: Application to Formosat-2 images

This paper presents a new method developed for the atmospheric correction of the images that will be acquired by the Venμs satellite after its launch expected in early 2010. Every two days, the Venμs mission will provide 10 m resolution images of 50 sites, in 12 narrow spectral bands ranging from 415 nm to 910 nm. The sun-synchronous Venμs orbit will have a 2-day repeat cycle, and the images of a given site will always be acquired from the same place, at the same local hour, with constant observation angles. Thanks to these characteristics, the directional effects will be considerably reduced since only the solar angles will slowly vary with time.The algorithm that will be implemented for the atmospheric correction of Venμs data is being developed using both radiative transfer simulations and the actual data acquired by the Formosat-2 satellite. Because of its one-day sun-synchronous repeat cycle, Formosat-2 acquires images with a sun-viewing geometry close to the one Venμs will offer. With this geometry, reflectance time series are free from directional effects on the short term, a feature which reduces the number of unknowns to retrieve. The atmospheric corrections algorithm exploits this feature and the two following assumptions:

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Aerosol optical properties vary quickly with time but slowly with location.

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Surface reflectances vary quickly with location but slowly with time.

Consequently, the top of atmosphere reflectance short term variations (10 to 15 days) are mainly due to the variations of aerosol optical properties, and it is thus possible to use these variations to characterise the atmospheric aerosols and to retrieve surface reflectances.This paper first describes the aerosol inversion method we developed and its results when applied to simulations. In the second part, we show the first tests of the method against three data sets acquired by Formosat-2 images with constant observation angles. Aeronet sun photometers measurements were available on all sites. Formosat-2 estimates of optical thickness compare favourably with Aeronet in situ measurements, leading to a noticeable improvement of the smoothness of time series of surface reflectances after atmospheric correction.     

Localization of an algebraic hypersurface by the exclusion algorithm

We describe a new algorithm for the localization of an algebraic hypersurfaceV inR ⁿ or Cⁿ. This algorithm computes a decreasing sequence of closed sets whose intersection isV. In the particular case of an hypersurface without any point at infinity, the notion of the asymptotic cone is used to determine a compact set containing this hypersurface. We give also a numerical version of this algorithm.     

Diagnosis of contagious bovine pleuropneumonia: problems and recent developments

While it is easy to diagnose contagious bovine pleuropneumonia (CBPP) in an animal in the acute clinical stage, subacute and chronic forms are more difficult to diagnose. Recourse to laboratory tests is essential to confirm any suspicion of CBPP. As standard diagnostic procedures (isolation, culture, biochemical tests, serological tests) are lacking in specificity and sensitivity, improvements are needed. Progress in molecular biology techniques has led to new tests, among which are the polymerase chain reaction (PCR) and the enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies. Application of these techniques to CBPP offers a number of advantages, and has considerably enhanced the specificity and sensitivity of diagnosis.