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排序方式: 共有369条查询结果,搜索用时 15 毫秒
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Dalal Bakhos-Douaihy Elie Seaayfan Sylvie Demaretz Martin Komhoff Kamel Laghmani 《International journal of molecular sciences》2021,22(4)
Mutations in the Na-K-2Cl co-transporter NKCC2 lead to type I Bartter syndrome, a life-threatening kidney disease. We previously showed that export from the ER constitutes the limiting step in NKCC2 maturation and cell surface expression. Yet, the molecular mechanisms involved in this process remain obscure. Here, we report the identification of chaperone stress 70 protein (STCH) and the stress-inducible heat shock protein 70 (Hsp70), as two novel binding partners of the ER-resident form of NKCC2. STCH knock-down increased total NKCC2 expression whereas Hsp70 knock-down or its inhibition by YM-01 had the opposite effect. Accordingly, overexpressing of STCH and Hsp70 exerted opposite actions on total protein abundance of NKCC2 and its folding mutants. Cycloheximide chase assay showed that in cells over-expressing STCH, NKCC2 stability and maturation are heavily impaired. In contrast to STCH, Hsp70 co-expression increased NKCC2 maturation. Interestingly, treatment by protein degradation inhibitors revealed that in addition to the proteasome, the ER associated degradation (ERAD) of NKCC2 mediated by STCH, involves also the ER-to-lysosome-associated degradation pathway. In summary, our data are consistent with STCH and Hsp70 having differential and antagonistic effects with regard to NKCC2 biogenesis. These findings may have an impact on our understanding and potential treatment of diseases related to aberrant NKCC2 trafficking and expression. 相似文献
5.
Jialing Liu Yadav V. Sehgal H. Olson J.M. Haifeng Liu Elia N. 《Automatic Control, IEEE Transactions on》2008,53(8):1817-1825
In this paper, we study the phase transition behavior emerging from the interactions among multiple agents in the presence of noise. We propose a simple discrete-time model in which a group of non-mobile agents form either a fixed connected graph or a random graph process, and each agent, taking bipolar value either +1 or -1, updates its value according to its previous value and the noisy measurements of the values of the agents connected to it. We present proofs for the occurrence of the following phase transition behavior: At a noise level higher than some threshold, the system generates symmetric behavior (vapor or melt of magnetization) or disagreement; whereas at a noise level lower than the threshold, the system exhibits spontaneous symmetry breaking (solid or magnetization) or consensus. The threshold is found analytically. The phase transition occurs for any dimension. Finally, we demonstrate the phase transition behavior and all analytic results using simulations. This result may be found useful in the study of the collective behavior of complex systems under communication constraints. 相似文献
6.
Mickaël Daniel Yves Lionel Elie Thomas Philippe Claude 《Sensors and actuators. A, Physical》2008,147(1):263-272
This paper introduces the conceptual architecture of a fully integrated, truly self-powered structural health monitoring (SHM) scheme. The challenge here is to power an array of numerous distributed actuators and sensors as well as wireless data transmission modules without recurring to heavy and costly wiring. Based on microgenerators which directly convert ambient mechanical energy into electrical energy, using the synchronized switch harvesting (SSH) method, the proposed solution allows avoiding the periodic replacement or reloading of batteries. This addresses environmental and economic issues at the same time, knowing that such elements are heavy, polluting and might be installed in rather inaccessible locations. Indeed, especially in airborne structures saving weight and maintenance cost is of priority importance.Previous work showed that such microgenerators provide a stand-alone power source, whose performances meet the requirements of autonomous wireless transmitters (AWTs) that comprise an acoustic Lamb wave's actuator and a radio frequency (RF) emitter (D. Guyomar, Y. Jayet, L. Petit, E. Lefeuvre, T. Monnier, C. Richard, M. Lallart, Synchronized switch harvesting applied to self-powered smart systems: Piezoactive microgenerators for autonomous wireless transmitters, Sens Actuators A: Phys. 138 (1) (2007) 151–160, doi:10.1016/j.sna.2007.04.009). Following this work, the present contribution presents a further step towards the integration of the SHM technique. It shows the ability of our microgenerators to provide enough energy to give logical autonomy to each self-powered sensing node, named autonomous wireless receiver (AWR), and thus to provide some local (decentralized) pre-processing ability to the SHM system.A preliminary design of the device using off-the-shelf electronics and surface mounted piezoelectric patches will be presented. Since the existence of a positive energy balance between the harvesting capabilities of the SSH technique and the energy requirements of the proposed device will be proved, the system formed by the combination of the AWR with the previously developed AWT, is a proof of concept of truly self-powered smart systems for damage detection in simple structures, setting apart application-specific optimization or miniaturization concerns that will be addressed in future works. 相似文献
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Foehn Philipp Brescianini Dario Kaufmann Elia Cieslewski Titus Gehrig Mathias Muglikar Manasi Scaramuzza Davide 《Autonomous Robots》2022,46(1):307-320
Autonomous Robots - This paper presents a novel system for autonomous, vision-based drone racing combining learned data abstraction, nonlinear filtering, and time-optimal trajectory planning. The... 相似文献
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Giuseppe Antonio Elia Krystan Marquardt Katrin Hoeppner Sebastien Fantini Rongying Lin Etienne Knipping Willi Peters Jean‐Francois Drillet Stefano Passerini Robert Hahn 《Advanced materials (Deerfield Beach, Fla.)》2016,28(35):7564-7579
A critical overview of the latest developments in the aluminum battery technologies is reported. The substitution of lithium with alternative metal anodes characterized by lower cost and higher abundance is nowadays one of the most widely explored paths to reduce the cost of electrochemical storage systems and enable long‐term sustainability. Aluminum based secondary batteries could be a viable alternative to the present Li‐ion technology because of their high volumetric capacity (8040 mAh cm?3 for Al vs 2046 mAh cm?3 for Li). Additionally, the low cost aluminum makes these batteries appealing for large‐scale electrical energy storage. Here, we describe the evolution of the various aluminum systems, starting from those based on aqueous electrolytes to, in more details, those based on non‐aqueous electrolytes. Particular attention has been dedicated to the latest development of electrolytic media characterized by low reactivity towards other cell components. The attention is then focused on electrode materials enabling the reversible aluminum intercalation‐deintercalation process. Finally, we touch on the topic of high‐capacity aluminum‐sulfur batteries, attempting to forecast their chances to reach the status of practical energy storage systems. 相似文献
9.
Corrosion of steel in reinforced concrete structures is a recurrent problem affecting civil engineering structures and costing the world billions of dollars per year. This physical phenomenon mainly results from chloride ingress or concrete carbonation. Corrosion can be diagnosed through a nondestructive method such as half-cell potential measurements. The present paper studies this method on a reinforced concrete wall containing eighteen unconnected steel bars and subjected to chloride-induced macrocell corrosion. Three corrosion systems with different configurations of connections between the steel bars are generated, involving three different anode-to-cathode surface ratios. Then, half-cell potential variations are observed versus macrocell corrosion current. The results lead to a critical discussion regarding the physical relevance of the usual potential threshold method to detect corroding rebars in reinforced concrete structures. In addition, the experiments demonstrate that electrical continuity between reinforcing steel bars is not necessary to get meaningful information about the macrocell corrosion system. At last, the paper show that the electric field (potential gradient) relative to a macrocell corrosion system may be measured by connecting the measurement system (reference electrode?+?voltmeter) to any electrochemical system in electrolytic contact with the concrete. 相似文献
10.
In this paper, the problem of residual variance estimation is examined. The problem is analyzed in a general setting which covers non-additive heteroscedastic noise under non-iid sampling. To address the estimation problem, we suggest a method based on nearest neighbor graphs and we discuss its convergence properties under the assumption of a Hölder continuous regression function. The universality of the estimator makes it an ideal tool in problems with only little prior knowledge available. 相似文献