半透明物体漫散射效果的实时绘制与材质编辑

半透明物体透明效果的真实感绘制是近年来研究的热点,提出一种针对半透明物体漫散射效果的实时真实感绘制与材质动态编辑方法--基于双向表面散射反射率函数(BSSRDF)的Dipole近似.通过主元分析将Dipole近似中的漫散射材质甬数分解为与形状相关甬数和与半透明材质相关函数的乘积形式;利用该分解表示,在预辐射传输的实时真实感绘制框架下,通过对散射传输的预计算来实现在多种光源环境下对半透明物体材质的实时编辑.此外,还提出一种对预计算辐射传输数据在空域上进行二次小波压缩的方法,利用表面点在空间分布位置的相关性,在保证绘制质量的前提下,大大压缩了数据,提升了绘制效率.实验结果表明,文中方法可以生成具有高度真实感的半透明效果并保证实时的绘制速度.     

Fluorescent Amino Acid Initiated de novo Cyclic Peptides for the Label-Free Assessment of Cell Permeability**

The major obstacle in applying peptides to intracellular targets is their low inherent cell permeability. Standard approaches to attach a fluorophore (e. g. FITC, TAMRA) can change the physicochemical properties of the parent peptide and influence their ability to penetrate and localize in cells. We report a label-free strategy for evaluating the cell permeability of cyclic peptide leads. Fluorescent tryptophan analogues 4-cyanotryptophan (4CNW) and β-(1-azulenyl)-L-alanine (AzAla) were incorporated into in vitro translated macrocyclic peptides by initiator reprogramming. We then demonstrate these efficient blue fluorescent emitters are good tools for monitoring peptide penetration into cells.     

Kernel density estimation based on Ripley’s correction

In this paper, we investigate a technique inspired by Ripley’s circumference method to correct bias of density estimation of edges (or frontiers) of regions. The idea of the method was theoretical and difficult to implement. We provide a simple technique – based of properties of Gaussian kernels – to efficiently compute weights to correct border bias on frontiers of the region of interest, with an automatic selection of an optimal radius for the method. We illustrate the use of that technique to visualize hot spots of car accidents and campsite locations, as well as location of bike thefts.     

Initial Molecular Recognition Steps of McjA Precursor during Microcin J25 Lasso Peptide Maturation

Microcin J25 (MccJ25) has emerged as an excellent model to understand the maturation of ribosomal precursor peptides into the entangled lasso fold. MccJ25 biosynthesis relies on the post‐translational modification of the precursor McjA by the ATP‐dependent protease McjB and the lactam synthetase McjC. Here, using NMR spectroscopy, we showed that McjA is an intrinsically disordered protein without detectable conformational preference, which emphasizes the active role of the maturation machinery on the three‐dimensional folding of MccJ25. We further showed that the N‐terminal region of the leader peptide is involved in interaction with both maturation enzymes and identified a predominant interaction of V43–S55 in the core McjA sequence with McjC. Moreover, we demonstrated that residues K23–Q34 in the N‐terminal McjA leader peptide tend to adopt a helical conformation in the presence of membrane mimics, implying a role in directing McjA to the membrane in the vicinity of the lasso synthetase/export machinery. These data provide valuable insights into the initial molecular recognition steps in the MccJ25 maturation process.     

Quantitative analysis of mitoxantrone by surface-enhanced resonance Raman scattering

Mitoxantrone is an anticancer agent for which it is important to know the concentration in blood during therapy. Current methods of analysis are cumbersome, requiring a pretreatment stage. A method based on surface-enhanced resonance Raman scattering (SERRS) has been developed using a flow cell and silver colloid as the SERRS substrate. It is simple, sensitive, fast, and reliable. Both blood plasma and serum can be analyzed directly, but fresh serum is preferred here due to reduced fluorescence in the clinical samples available. Fluorescence is reduced further by the dilution of the serum in the flow cell and by quenching by the silver of surface-adsorbed material. The effectiveness of the latter process is dependent on the contact time between the serum and the silver. The linear range encompasses the range of concentrations detected previously in patient samples using HPLC methods. In a comparative study of a series of samples taken from a patient at different times, there is good agreement between the results obtained by HPLC and SERRS with no significant difference between them at the 95% limit. The limit of detection in serum using the final optimized procedure for SERRS was 4.0 x 10(-11) M (0.02 ng/mL) mitoxantrone. The ease with which the SERRS analysis can be carried out makes it the preferred choice of technique for mitoxantrone analysis.     

Multilevel monolithic inductors in silicon technology

Multilevel monolithic inductors implemented in standard BiCMOS technology are presented. Use of top metal layers shunted with vias provides Q values approaching 10 at 2.4 GHz and above 6 at 900 MHz for a 2 nH inductor. There is no modification to the conventional wiring metallurgy and no need for extra processing steps