Electrochemical Quantification of Neurotransmitters in Single Live Cell Vesicles Shows Exocytosis is Predominantly Partial

Exocytosis plays an essential role in the communication between cells in the nervous system. Understanding the regulation of neurotransmitter release during exocytosis and the amount of neurotransmitter content that is stored in vesicles is of importance, as it provides fundamental insights to understand how the brain works and how neurons elicit a certain behavior. In this minireview, we summarize recent progress in amperometric measurements for monitoring exocytosis in single cells and electrochemical cytometry measurements of vesicular neurotransmitter content in individual vesicles. Important steps have increased our understanding of the different mechanisms of exocytosis. Increasing evidence is firmly establishing that partial release is the primary mechanism of release in multiple cell types.     

Psychological testing in evaluation of child sexual abuse

In order to assess the utility of psychological testing in evaluating allegations of child sexual abuse, the empirical literature is reviewed in an attempt to answer two questions. First, are there systematic and significant differences on psychological tests between sexually abused and nonabused children? Second, are these differences on psychological testing a direct result of sexual abuse, or are they a result of other coexisting factors that might cause psychological distress? Cognitive measures, personality inventories, symptomatology checklists, and projective tests with sexually abused children are reviewed. While a variety of standardized instruments have been administered to sexually abused children, relatively few have been utilized in empirical studies. Findings have been mixed, with stronger differences between sexually abused and nonabused children generally found on measures completed by parents than on measures administered directly to children. Sexually abused children often fall between nonabused and psychiatric groups. Use of measures specific to sexual abuse is advocated. While psychological tests may currently have limited use in validating suspected sexual abuse, they may be extremely useful in the clinical treatment of the child.     

An efficient algorithm for characteristic tracking on two-dimensional triangular meshes

Tracking characteristics on unstructured meshes is an important part of many numerical methods in computational fluid mechanics. In this paper, we propose an efficient algorithm for characteristic tracking on two-dimensional unstructured triangular meshes. Numerical experiments, including an example for applying this algorithm with the Eulerian-Lagrangian localized adjoint method (ELLAM) to solve a convection-dominated convection-diffusion problem, are presented to demonstrate the efficiency of this algorithm.     

Separation of serotonin from catechols by capillary zone electrophoresis with electrochemical detection

Capillary zone electrophoresis with electrochemical detection is demonstrated with columns having only 9-microns inner diameter. Amperometric detection limits of 0.7 amol are reported for serotonin. The difficult problem of resolving serotonin and dopamine--two neurotransmitters of interest having similar electrophoretic mobilities--is addressed by chemical means to improve selectivity. These include buffer modification with 2-propanol and a system employing borate complexation of the catechol in combination with sodium dodecyl sulfate micelles.     

Time series analysis of wind speed using VAR and the generalized impulse response technique

This research examines the interdependence in time series wind speed data measured in the same location at four different heights. A multiple-equation system known as a vector autoregression is proposed for characterizing the time series dynamics of wind. Additionally, the recently developed method of generalized impulse response analysis provides insight into the cross-effects of the wind series and their responses to shocks. Findings are based on analysis of contemporaneous wind speed time histories taken at 13, 33, 70 and 160 ft above ground level with a sampling rate of 10 Hz. The results indicate that wind speeds measured at 70 ft was the most variable. Further, the turbulence persisted longer at the 70-ft measurement than at the other heights. The greatest interdependence is observed at 13 ft. Gusts at 160 ft led to the greatest persistence to an “own” shock and led to greatest persistence in the responses of the other wind series.     

Investigation of fusion reactions in palladium and titanium tritide using galvanostatic,coulometric, and hydrogen permeation techniques

We describe several electrochemical methods used to investigate the possibility of cold fusion phenomena in palladium and titanium tritide cathodes. We performed long-term (up to 77 days) electrolysis experiments with electrochemical cells of the University of Utah type at current densities as high as 1 A/cm², while monitoring neutron and tritium levels. With some cells, we pulsed the current to determine if neutron bursts would result. In another cell, we used titanium tritide as the cathode to determine if D-T reactions yielding neutrons would occur. In no instance were levels of neutrons or tritium significantly above background except in the titanium tritide cell where isotopic exchange, occcurring between the electrode and the electrolyte, resulted in significant tritium levels. We also combined x-ray photoelectron spectroscopy (XPS) and electrochemical hydrogen permeation experiments to determine the effectiveness of various Pd surface treatment procedures on the resultant electrochemical hydrogen absorption efficiency. Electroanalytical and thermal desorption/gas analysis techniques indicated the maximum loading of H in Pd was to a ratio of HPd=0.8.     

Power up! Using Digital Gaming Techniques to Enhance Library Instruction

ABSTRACT

Incorporating digital gaming into education is an increasingly popular topic in academia. Educators’ views of digital gaming (e.g., computer, video, and online games) have shifted from categorizing these games as an entertaining, yet pedagogically void activity to a promising medium that engages students in the learning process. With over 70% of college students enthusiastically playing digital games, educators are tapping into digital gaming as an effective means to teach millennials problem solving and communication skills. This article investigates gamer characteristics, motivational and engaging aspects of digital games, and the parallels between gaming and the information retrieval process. The authors build on this information to present approaches to incorporate digital gaming techniques into the library classroom since it is not feasible for all libraries to create instructional digital games.     

Detection of low levels of trichloroethylene vapor with Raman spectrometry

The response of a novel fiber-optic Raman probe to low levels of trichloroethylene (TCE) vapors is characterized. The detection limit of the current probe for TCE vapor is 34 mg/L, and the probe exhibits a fully reversible response. The probe uses an organic-polymer, low-density polyethylene to concentrate TCE vapors in the optical path of the fiber-optic Raman spectrometer. The relative standard deviation for measurement of 677 mg/L of TCE in the vapor is 0.3%.     

Degenerate Two-Phase Incompressible Flow IV: Local Refinement and Domain Decomposition

This is the fourth paper of a series in which we analyze mathematical properties and develop numerical methods for a degenerate elliptic-parabolic partial differential system which describes the flow of two incompressible, immiscible fluids in porous media. In this paper we describe a finite element approximation for this system on locally refined grids. This adaptive approximation is based on a mixed finite element method for the elliptic pressure equation and a Galerkin finite element method for the degenerate parabolic saturation equation. Both discrete stability and sharp a priori error estimates are established for this approximation. Iterative techniques of domain decomposition type for solving it are discussed, and numerical results are presented.