珍珠层粉/壳聚糖多孔支架的制备及分析测试

用负压真空冷冻干燥机在-20℃条件下冷冻干燥48h制备珍珠层粉/壳聚糖复合多孔支架。用扫描电子显微镜(SEM)观察支架表面形貌,通过X射线衍射分析(XRD)、傅里叶红外光谱(FTIR)等手段对材料进行表征测试,检测其降解性能,并将兔骨髓间充质干细胞与支架共培养检测材料细胞毒性。结果表明复合支架具有稳固的三维多孔结构,孔隙率最大至91.64%,孔径在100~300μm范围,降解性能适宜,且冷冻干燥制备方法对珍珠层粉的结构无明显破坏,并具有良好的生物相容性,有利于细胞的生长。     

The evaluation of a social adaptive website for cultural events

In this paper, we present an evaluation of a social adaptive website in the domain of cultural events, iCITY DSA, which provides information about cultural resources and events that promote the cultural heritage in the city of Turin. Using this evaluation, our objective was to investigate the actual usage of a social adaptive website, in an effort to discover the real behavior of users, the unforeseen correlations among user actions and the consequent interactive behavior, the accuracy of both system and social recommendations and their impact on the users themselves, and the role of tagging in the user modeling process. The major contributions of the paper are manifold: insights into user interactions with social adaptive systems; guidelines for future designs; evaluation of the tagging activity and tag meanings in relation to the application domain and thus their impact on the representation of the user model; and a demonstration of how a combination and interplay of evaluation methodologies (e.g., quantitative and qualitative) can enhance our comprehension of evaluation data.     

The canonical processes of a dramatized approach to information presentation

This paper describes the application “Carletto the spider” in terms of the mapping with the canonical processes of media production. “Carletto the spider” is a character-based guide to a historical site and implements the Dramatour approach for the design of drama-based interactive presentations. Dramatization makes presentations more engaging, thus improving the reception of the content by the user. The major technical issue of the approach is the segmentation of the presentation into audiovisual units that are edited on-the-fly in a way that guarantees dramatic continuity while adapting to the user response. We describe the workflow of the application and its mapping to the canonical processes of media production, envisaging possible standardizations for the application portability.     

Enhancing cultural recommendations through social and linked open data

In this article, we describe a hybrid recommender system (RS) in the artistic and cultural heritage area, which takes into account the activities on social media performed by the target user and her friends, and takes advantage of linked open data (LOD) sources. Concretely, the proposed RS (1) extracts information from Facebook by analyzing content generated by users and their friends; (2) performs disambiguation tasks through LOD tools; (3) profiles the active user as a social graph; (4) provides her with personalized suggestions of artistic and cultural resources in the surroundings of the user’s current location. The last point is performed by integrating collaborative filtering algorithms with semantic technologies in order to leverage LOD sources such as DBpedia and Europeana. Based on the recommended points of cultural interest, the proposed system is also able to suggest to the active user itineraries among them, which meet her preferences and needs and are sensitive to her physical and social contexts as well. Experimental results on real users showed the effectiveness of the different modules of the proposed recommender.

     

The HP1a disordered C terminus and chromo shadow domain cooperate to select target peptide partners

Drosophila melanogaster heterochromatin protein 1a (HP1a) is essential for compacted heterochromatin structure and the associated gene silencing. Its chromo shadow domain (CSD) is well known for binding to peptides that contain a PXVXL motif. Heterochromatin protein 2 (HP2) is a non-histone chromosomal protein that associates with HP1a in the pericentric heterochromatin, telomeres, and the fourth chromosome. Using NMR spectroscopy, fluorescence polarization, and site-directed mutagenesis, we identified an LCVKI motif in HP2 that binds to the HP1a CSD. The binding affinity of the HP2 fragment is approximately two orders of magnitude higher than that of peptides from PIWI (with a PRVKV motif), AF10 (with a PLVVL motif), or CG15356 (with LYPLL and LSIVA motifs). To delineate differential interactions of the HP1a CSD, we characterized its structure, backbone dynamics, and dimerization constant. We found that the dimerization constant is bracketed by the affinities of HP2 and PIWI, which dock to the same HP1a homodimer surface. This suggests that HP2, but not PIWI, interaction can drive the homodimerization of HP1a. Interestingly, the integrity of the disordered C-terminal extension (CTE) of HP1a is essential for discriminatory binding, whereas swapping the PXVXL motifs does not confer specificity. Serine phosphorylation at the peptide binding surface of the CSD is thought to regulate heterochromatin assembly. Glutamic acid substitution at these sites destabilizes HP1a dimers, but improves the interaction with both binding partners. Our studies underscore the importance of CSD dimerization and cooperation with the CTE in forming distinct complexes of HP1a.     

User model interoperability: a survey

Nowadays a large number of user-adaptive systems has been developed. Commonly, the effort to build user models is repeated across applications and domains, due to the lack of interoperability and synchronization among user-adaptive systems. There is a strong need for the next generation of user models to be interoperable, i.e. to be able to exchange user model portions and to use the information that has been exchanged to enrich the user experience. This paper presents an overview of the well-established literature dealing with user model interoperability, discussing the most representative work which has provided valuable solutions to face interoperability issues. Based on a detailed decomposition and a deep analysis of the selected work, we have isolated a set of dimensions characterizing the user model interoperability process along which the work has been classified. Starting from this analysis, the paper presents some open issues and possible future deployments in the area.     

Low Prostate Concentration of Lycopene Is Associated with Development of Prostate Cancer in Patients with High-Grade Prostatic Intraepithelial Neoplasia

Prostate cancer (PC) is a frequent male malignancy and represents the second most diagnosed cancer in men. Since pre-cancerous lesions, i.e., the high-grade prostatic intraepithelial neoplasia (HGPIN), can be detected years before progression to PC, early diagnosis and chemoprevention are targeted strategies to reduce PC rates. Animal studies have shown that lycopene, a carotenoid contained in tomatoes, is a promising candidate for the chemoprevention of PC. However, its efficacy in humans remains controversial. The present study aimed to investigate the relevance of plasma and prostate concentration of lycopene after a lycopene-enriched diet in patients diagnosed with HGPIN. Thirty-two patients diagnosed with HGPIN were administered a lycopene-enriched diet (20–25 mg/day of lycopene; through 30 g/day of triple concentrated tomato paste) for 6 months. A 6-month follow-up prostate biopsy assessed progression to PC. Patients were classified into three groups according to the histopathological features of the 6-month follow-up biopsy results: prostatitis; HGPIN and PC. PSA and plasma lycopene levels were measured before and after the dietary lycopene supplementation. Prostatic lycopene concentration was only assessed after the supplementation diet. Only prostatic lycopene concentration showed significant differences between the three groups (p = 0.03). Prostatic lycopene concentration below a 1 ng/mg threshold was associated with PC at 6-month follow-up biopsy (p = 0.003). We observed no overall benefits from a 6-month lycopene supplementation, as the rate of HGPIN progression to PC in our population (9/32, 28%) was similar to rates reported in the literature. Baseline PSA levels also showed no significant changes after a lycopene-enriched diet. Our findings point to prostatic lycopene concentration as a promising biomarker of PC. Further prospective longitudinal studies are needed to assess the prognostic role of prostatic lycopene in PC.     

Apropos sonification: a broad view of data as music and sound

Numbers have been identified with symbolic data forever. The profound association of both with acoustics, music, and sonic art from Pythagoras to current work is beyond reproach. Recently, sonification looks for ways to realize symbolic data (representing results or measurements) as well as “raw” data (signals, impulses, images, etc.) into compositions. In the strictest sense, everything in a computer is symbolic, that is, represented by 0s and 1s. In the arts, the digital age has broadened and enhanced the conceptual landscape not simply through its servitude to the creative process, but as its partner. However, there is a rich history of the use of data that no doubt has paved the way for many of today’s experiments including my own.     

Awakening to a New Dawn: Perspectives of the Future of School Psychology.

The Invitational Conference on the Future of School Psychology held at Indiana University in Fall 2002 included a number of paper presentations and group discussions. Toward the end of the conference, four participants were asked to present their reactions, reflecting input from a practitioner, a graduate student in school psychology, a faculty member at a university program educating school psychologists in the United States, and a school psychologist/faculty member at a university from a different country (Greece). The purpose of this article is to share their combined perspectives of the future of school psychology. This article also includes their suggestions for the future direction of school psychology, and encourages all school psychologists to awaken to a change in the field. The article considers the educational and mental health needs of students, and offers a prospectus for the field including (a) data-supported services; (b) group-focused practices; (c) collaboration and partnerships with professionals, families, and schools; and (d) multicultural considerations. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

How scales influence user rating behaviour in recommender systems

Many websites allow users to rate items and share their ratings with others, for social or personalisation purposes. In recommender systems in particular, personalised suggestions are generated by predicting ratings for items that users are unaware of, based on the ratings users provided for other items. Explicit user ratings are collected by means of graphical widgets referred to as ‘rating scales’. Each system or website normally uses a specific rating scale, in many cases differing from scales used by other systems in their granularity, visual metaphor, numbering or availability of a neutral position. While many works in the field of survey design reported on the effects of rating scales on user ratings, these, however, are normally regarded as neutral tools when it comes to recommender systems. In this paper, we challenge this view and provide new empirical information about the impact of rating scales on user ratings, presenting the results of three new studies carried out in different domains. Based on these results, we demonstrate that a static mathematical mapping is not the best method to compare ratings coming from scales with different features, and suggest when it is possible to use linear functions instead.