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Chemical composition of microalgae Heterochlorella luteoviridis and Dunaliella tertiolecta with emphasis on carotenoids
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Paula Alexandra Ribeiro Tafulo Raquel Barbosa Queirós Cristina Maria Delerue-Matos Maria Goreti Ferreira Sales 《Food research international (Ottawa, Ont.)》2010,43(6):1702-1709
The purpose of the present work is to determine the antioxidant capacity (AC) of 27 commercial beers. The AC indicates the degree of protection of a certain organism against oxidative damage provoked by reactive oxygen and nitrogen species.Assays were carried out by the following methods: (i) total radical trapping antioxidant parameter (TRAP); (ii) trolox equivalent antioxidant capacity (TEAC); (iii) trolox equivalent antioxidant capacity (DPPH); (iv) ferric-ion reducing antioxidant parameter (FRAP); (v) cupric reducing antioxidant capacity (CUPRAC); (vi) oxygen radical absorbance capacity (ORAC). Ascorbic acid (AA), gallic acid (GA) and trolox (TR) were used as standards.All beers showed antioxidant power, but a wide range of ACs was observed. The effect of several factors upon these differences was studied. Statistical differences were found between ACs of beers of different colours. ORAC method provided always higher experimental ACs, of significant statistical differences to other assays. 相似文献
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Felismina T.C. Moreira M. Goreti F. Sales 《Materials science & engineering. C, Materials for biological applications》2011,31(5):1121-1128
A new man-tailored biomimetic sensor for Chlorpromazine host-guest interactions and potentiometric transduction is presented. The artificial host was imprinted within methacrylic acid, 2-vinyl pyridine and 2-acrylamido-2-methyl-1-propanesulfonic acid based polymers. Molecularly imprinted particles were dispersed in 2-nitrophenyloctyl ether and entrapped in a poly(vinyl chloride) matrix. Slopes and detection limits ranged 51–67 mV/decade and 0.46–3.9 μg/mL, respectively, in steady state conditions. Sensors were independent from the pH of test solutions within 2.0–5.5. Good selectivity was observed towards oxytetracycline, doxytetracycline, ciprofloxacin, enrofloxacin, nalidixic acid, sulfadiazine, trimethoprim, glycine, hydroxylamine, cysteine and creatinine. Analytical features in flowing media were evaluated on a double-channel manifold, with a carrier solution of 5.0 × 10?2 mol/L phosphate buffer. Near-Nernstian response was observed over the concentration range 1.0 × 10?4 to 1.0 × 10?2 mol/L. Average slopes were about 48 mV/decade. The sensors were successfully applied to field monitoring of CPZ in fish samples, offering the advantages of simplicity, accuracy, automation feasibility and applicability to complex samples. 相似文献
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A 3D-mirror synthetic receptor for ciprofloxacin host–guest interactions and potentiometric transduction is presented. The host cavity was shaped on a polymeric surface assembled with methacrylic acid or 2-vinyl pyridine monomers by radical polymerization. Molecularly imprinted particles were dispersed in 2-nitrophenyl octyl ether and entrapped in a poly(vinyl chloride) matrix. The sensors exhibited a near-Nernstian response in steady state evaluations. Slopes and detection limits ranged from 26.8 to 50.0 mV decade−1 and 1.0 × 10−5 to 2.7 × 10−5 mol L−1, respectively. Good selectivity was observed for trimethoprim, enrofloxacin, tetracycline, cysteine, galactose, hydroxylamine, creatinine, ammonium chloride, sucrose, glucose, sulphamerazine and sulfadiazine. The sensors were successfully applied to the determination of ciprofloxacin concentrations in fish and in pharmaceuticals. The method presented offered the advantages of simplicity, accuracy, applicability to colored and turbid samples and automation feasibility, as well as confirming the use of molecularly imprinted polymers as ionophores for organic ion recognition in potentiometric transduction. 相似文献
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M. Fátima Barroso Aurora Silva Sandra Ramos M.T. Oliva-Teles Cristina Delerue-Matos M. Goreti F. Sales M.B.P.P. Oliveira 《Food chemistry》2009
Macro (Ca, Mg, K, Na) and micromineral (Fe, Zn, Cu) composition of 39 waters was analysed. Determinations were made by atomic flame spectrophotometry for macrominerals and electrothermic atomisation in graphite furnace for microminerals. 相似文献
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Supporting group decision‐making when the decision makers are spread around the world is a complex process. The mechanisms of automated negotiation, such as argumentation, can be used in Ubiquitous Group Decision Support Systems (UbiGDSS) to help decision makers find a solution based on their preferences. However, the decision‐making process is much more than just a simple criteria and alternative analysis. There are many cognitive and affective issues that affect the outcome, and these issues should not be ignored; otherwise, the quality of the decision could be compromised. In this paper, we detail an UbiGDSS architecture and explore 2 cognitive and affective methods that are essential to the group decision‐making process. We explain how agents can reason about self‐expertise and other decision makers' credibility, and how agents can verify and react to tendencies throughout the decision‐making process. We intend agents to achieve higher quality and more consensual decisions. In any simulation environment that we tested, agents that analysed credibility, expertise, and/or analysed tendencies always achieved a higher consensus compared to agents that used neither of the proposed methods. Likewise, agents that used neither of the proposed methods or only performed tendencies analysis obtained the worst average satisfaction levels for each simulation environment. 相似文献
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J. Rafaela L. Guerreiro M. Goreti F. Sales Felismina T. C. Moreira Tânia S. R. Rebelo 《European Food Research and Technology》2011,232(1):39-50
The indiscriminate use of antibiotics in food-producing animals has received increasing attention as a contributory factor
in the international emergence of antibiotic-resistant bacteria (Woodward in Pesticide, veterinary and other residues in food,
CRC Press, Boca Raton, 2004). Numerous analytical methods for quantifying antibacterial residues in edible animal products
have been developed over years (Woodward in Pesticide, veterinary and other residues in food, CRC Press, Boca Raton, 2004;
Botsoglou and Fletouris in Handbook of food analysis, residues and other food component analysis, Marcel Dekker, Ghent, 2004).
Being Amoxicillin (AMOX) one of those critical veterinary drugs, efforts have been made to develop simple and expeditious
methods for its control in food samples. In literature, only one AMOX-selective electrode has been reported so far. In that
work, phosphotungstate:amoxycillinium ion exchanger was used as electroactive material (Shoukry et al. in Electroanalysis
6:914–917, 1994). Designing new materials based on molecularly imprinted polymers (MIPs) which are complementary to the size
and charge of AMOX could lead to very selective interactions, thus enhancing the selectivity of the sensing unit. AMOX-selective
electrodes used imprinted polymers as electroactive materials having AMOX as target molecule to design a biomimetic imprinted
cavity. Poly(vinyl chloride), sensors of methacrylic acid displayed Nernstian slopes (60.7 mV/decade) and low detection limits
(2.9 × 10−5 mol/L). The potentiometric responses were not affected by pH within 4–5 and showed good selectivity. The electrodes were
applied successfully to the analysis of real samples. 相似文献
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Sara Monteiro-Reis Carina Carvalho-Maia Genevieve Bart Seppo J. Vainio Juliana Pedro Eunice R. Silva Goreti Sales Rui Henrique Carmen Jernimo 《International journal of molecular sciences》2021,22(6)
Secreted extracellular vesicles (EVs) are heterogeneous cell-derived membranous granules which carry a large diversity of molecules and participate in intercellular communication by transferring these molecules to target cells by endocytosis. In the last decade, EVs’ role in several pathological conditions, from etiology to disease progression or therapy evasion, has been consolidated, including in central nervous system (CNS)-related disorders. For this review, we performed a systematic search of original works published, reporting the presence of molecular components expressed in the CNS via EVs, which have been purified from plasma, serum or cerebrospinal fluid. Our aim is to provide a list of molecular EV components that have been identified from both nonpathological conditions and the most common CNS-related disorders. We discuss the methods used to isolate and enrich EVs from specific CNS-cells and the relevance of its components in each disease context. 相似文献