Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts

Cancer-associated fibroblasts (CAFs) are one of the most abundant and critical components of the tumor stroma. CAFs can impact many important steps of cancerogenesis and may also influence treatment resistance. Some of these effects need the direct contact of CAFs and cancer cells, while some involve paracrine signals. In this study, we investigated the ability of head and neck squamous cell carcinomas (HNSCC) patient-derived CAFs to promote or inhibit the colony-forming ability of HNSCC cells. The effect of cisplatin on this promoting or inhibiting influence was also studied. The subsequent analysis focused on changes in the expression of genes associated with cancer progression. We found that cisplatin response in model HNSCC cancer cells was modified by coculture with CAFs, was CAF-specific, and different patient-derived CAFs had a different “sensitizing ratio”. Increased expression of VEGFA, PGE2S, COX2, EGFR, and NANOG in cancer cells was characteristic for the increase of resistance. On the other hand, CCL2 expression was associated with sensitizing effect. Significantly higher amounts of cisplatin were found in CAFs derived from patients who subsequently experienced a recurrence. In conclusion, our results showed that CAFs could promote and/or inhibit colony-forming capability and cisplatin resistance in HNSCC cells via paracrine effects and subsequent changes in gene expression of cancer-associated genes in cancer cells.     

Multiple spatial sounds in hierarchical menu navigation for visually impaired computer users

This paper describes a user study on the benefits and drawbacks of simultaneous spatial sounds in auditory interfaces for visually impaired and blind computer users. Two different auditory interfaces in spatial and non-spatial condition were proposed to represent the hierarchical menu structure of a simple word processing application. In the horizontal interface, the sound sources or the menu items were located in the horizontal plane on a virtual ring surrounding the user’s head, while the sound sources in the vertical interface were aligned one above the other in front of the user. In the vertical interface, the central pitch of the sound sources at different elevations was changed in order to improve the otherwise relatively low localization performance in the vertical dimension. The interaction with the interfaces was based on a standard computer keyboard for input and a pair of studio headphones for output. Twelve blind or visually impaired test subjects were asked to perform ten different word processing tasks within four experiment conditions. Task completion times, navigation performance, overall satisfaction and cognitive workload were evaluated. The initial hypothesis, i.e. that the spatial auditory interfaces with multiple simultaneous sounds should prove to be faster and more efficient than non-spatial ones, was not confirmed. On the contrary—spatial auditory interfaces proved to be significantly slower due to the high cognitive workload and temporal demand. The majority of users did in fact finish tasks with less navigation and key pressing; however, they required much more time. They reported the spatial auditory interfaces to be hard to use for a longer period of time due to the high temporal and mental demand, especially with regards to the comprehension of multiple simultaneous sounds. The comparison between the horizontal and vertical interface showed no significant differences between the two. It is important to point out that all participants were novice users of the system; therefore it is possible that the overall performance could change with a more extensive use of the interfaces and an increased number of trials or experiments sets. Our interviews with visually impaired and blind computer users showed that they are used to sharing their auditory channel in order to perform multiple simultaneous tasks such as listening to the radio, talking to somebody, using the computer, etc. As the perception of multiple simultaneous sounds requires the entire capacity of the auditory channel and total concentration of the listener, it does therefore not enable such multitasking.     

A system for efficient motor learning using multimodal augmented feedback

Numerous studies have established that using various forms of augmented feedback improves human motor learning. In this paper, we present a system that enables real-time analysis of motion patterns and provides users with objective information on their performance of an executed set of motions. This information can be used to identify individual segments of improper motion early in the learning process, thus preventing improperly learned motion patterns that can be difficult to correct once fully learned. The primary purpose of the proposed system is to serve as a general tool in the research on impact of different feedback modalities on the process of motor learning, for example, in sports or rehabilitation. The key advantages of the system are high-speed and high-accuracy tracking, as well as its flexibility, as it supports various types of feedback (auditory and visual, concurrent or terminal). The practical application of the proposed system is demonstrated through the example of learning a golf swing.     

Historical analysis of pharmacoeconomic terms

Scientometrics - Pharmacoeconomy is a young interdisciplinary science at the intersection of pharmacy, medicine and economics, focusing on the social aspects and implications of different types of...     

Simulation of liquid micro-jet in free expanding high-speed co-flowing gas streams

We present the development of an experimentally validated computational fluid dynamics model for liquid micro jets. Such jets are produced by focusing hydrodynamic momentum from a co-flowing sheath of gas on a liquid stream in a nozzle. The numerical model based on laminar two-phase, Newtonian, compressible Navier–Stokes equations is solved with finite volume method, where the phase interface is treated by the volume of fluid approach. A mixture model of the two-phase system is solved in axisymmetry using?~?300,000 finite volumes, while ensuring mesh independence with the finite volumes of the size 0.25 µm in the vicinity of the jet and drops. The numerical model is evaluated by comparing jet diameters and jet lengths obtained experimentally and from scaling analysis. They are not affected by the strong temperature and viscosity changes in the focusing gas while expanding at nozzle outlet. A range of gas and liquid-operating parameters is investigated numerically to understand their influence on the jet performance. The study is performed for gas and liquid Reynolds numbers in the range 17–1222 and 110–215, and Weber numbers in the range 3–320, respectively. A reasonably good agreement between experimental and scaling results is found for the range of operating parameters never tackled before. This study provides a basis for further computational designs as well as adjustments of the operating conditions for specific liquids and gases.     

Prospective memory in Parkinson disease across laboratory and self-reported everyday performance.

Prospective memory is a complex cognitive construct ubiquitous in everyday life that is thought to sometimes rely on executive skills commonly affected by Parkinson’s disease (PD). The present study investigated the effect of PD on prospective memory tasks with varying demand on executive control processes, namely on the amount of strategic attentional monitoring required for intention retrieval. Individuals with PD but without dementia and healthy adults performed laboratory event-based prospective memory tasks that varied in whether strategic attentional monitoring (nonfocal condition) or spontaneous processes (focal condition) were primarily involved in intention retrieval. Participants also completed a questionnaire rating their frequency of prospective memory failures in everyday life for both self-cued and environment-cued tasks. PD participants performed worse than non-PD participants in the nonfocal, but not focal, condition of the laboratory task. They also reported more everyday failures than non-PD participants for self-cued, but not environment-cued, prospective memory tasks. Thus, nondemented individuals with PD are preferentially impaired on prospective memory tasks for which higher levels of executive control are needed to support intention retrieval. This pattern is consistent across laboratory and reported real-world performance. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

INACT—INDECT Advanced Image Cataloguing Tool

Child pornography possession and distribution are crimes which are prosecuted in most countries around the world. In some cases the law is so strict that even police forces are not allowed to gather and catalogue evidence for future reference. This paper presents an innovative solution to this problem. The authors present tools for cataloguing high- and low-level metadata of the evidence material. Furthermore, a tool for fast and accurate search for such evidence in suspects’ file systems is proposed.     

Digital control of a tank system

This paper describes the laboratory environment for experiments in digital control of a coupled tank system. The control process consists of upper and lower containers with draining orifices. As the cross section of the lower container varies with the level, the dynamic of the process changes extensively with the selected steady-state operating level. Measurements of the tank levels are available, while the flow rate into the upper tank is forced by a pump driven by an electric motor. A nonlinear model is employed to study various features of the process. The control strategy is to stabilize the level in the lower tank by adjustment of the flow rate into the upper tank. An IBM AT, equipped with a universal analog input/output board is used to control the process. The interactive real-time software frame, available under MS-DOS, uses selectable operating modes to analyze the performance of different control algorithms. A digital state-feedback algorithm is presented as an illustration