Characterization of the binding site for inhibitors of the HPV11 E1-E2 protein interaction on the E2 transactivation domain by photoaffinity labeling and mass spectrometry

An indandione-containing class of inhibitors abrogates DNA replication of human papillomavirus (HPV) types 6 and 11 by binding reversibly to the transactivation domain (TAD) of the viral E2 protein and inhibiting its interaction with the viral E1 helicase. To locate the binding site of this class of protein-protein interaction inhibitors, a benzophenone derivative was used to generate an irreversibly labeled E2-TAD polypeptide. The single site of covalent modification of the E2-TAD was identified by proteolytic digestions using trypsin, LysC, and V8 proteases and characterization of the resulting peptides by LC-MS procedures. Through this methodology, the benzophenone attachment point was located at the terminal methyl of residue Met101. Evidence further pinpointed the site of photoaffinity attachment to the terminal carbon atom, which is significant in providing a definitive example of the ability to locate photoinduced cross-linking to a polypeptide with atomic resolution using solely mass spectrometric detection. The location of the inhibitor binding site vis-à-vis the Glu39 and Glu100 residues sensitive to mutation for HPV 11 E2-TAD is discussed in relation to the crystal structure of the E2-TAD from the related HPV type 16.     

Leistungsfähigkeit von Betonen mit Flugasche

Performance Capacity of Concrete containing Fly Ash The conception to take into account the addition of fly ash to structural concrete presented in the German Standard DIN 1045‐2 should guarantee a high resistance of concrete structures against corrosive attack. In this context, criteria such as compressive and flexural strength of concrete, modulus of elasticity, bond strength, shrinkage and creep as well as the pore structure are focused in this paper. The obtained results on concretes containing a maximum of fly ash according to DIN 1045‐2 compared with concretes produced only with ordinary Portland cement show similar strength values at the age of 28 days while the strength development is somewhat lower for the fly ash concretes. However, at the age of one year the strength of fly ash concretes in particular also the flexural strength is considerably higher. Shrinkage and creep of the fly ash concrete is lower resulting in an overall reduced risk of shrinkage and thermal cracking.     

Prevalence of carbohydrate metabolism disorders in free-living females of capable age in the city of Baku

AIM: The study of prevalence of carbohydrate metabolism disorders (CHMD) in females. MATERIALS AND METHODS: A representative sample of 20-59-year-old female population of the city of Baku was examined using age-specific standardized index, WHO tests and criteria. RESULTS: CHMD occurred rather frequently. Tolerance to glucose was abnormal in 50% of the examinees. Diabetes mellitus affected 20-29-year-old women 4 times more frequently than poor glucose tolerance. An opposite trend was observed for 40-49-year-old women. CONCLUSION: Relevant prophylactic measures are required for female Baku population.     

Non-cartilaginous metaphyseal cysts in Legg-Calvé-Perthes disease: report of a case

Discrete metaphyseal radiolucent lesions, often termed "metaphyseal cysts", are a frequent finding in Legg-Calvé-Perthes disease. Recent literature emphasizes that such lesions represent ectopic physeal cartilage. This paper reports a case in which biopsy of the metaphyseal lesion indicated that no cartilage was present.     

On the photochemical phenomenon in TATB

Abstract

TATB (1,3,5 triaminotrinitrobenzene) is a yellow compound that slowly changes to green upon exposure to sunlight. IR, UV-Visible, and ESCA data pertaining to this phenomenon are presented. It is shown that various types of irradiation can induce NO₂ loss, radical formation, or secondary amine production in TATB. Molecular orbital calculations are used to dispute the previously assigned structure of the radical in irradiated TATB.     

Improvement in resource utilization after development of a clinical pathway for patients with pressure ulcers

The aim of this work was to assess the relationship between GH-binding protein (GHBP) and leptin. Both peptides are nutritionally regulated, but the recent implication of a role for leptin in the GH axis requires further study. To avoid the sexual dimorphism in leptin values, we performed leptin standardization according to gender (SD score-leptin). The relationship between SD score-leptin and GHBP was studied in 128 adults with different nutritional status [8 groups according to body mass index (BMI)], ranging from severely underweight anorexia nervosa to highly morbid obesity. Both GHBP and SD score-leptin significantly increased according to BMI within the range from 18-27 kg/m2, whereas no significant differences were found among underweight groups (BMI, < 18 kg/m2) or among obesity grades (BMI, > 27 kg/m2). We found a strong correlation between GHBP and SD score-leptin (r = 0.8; P < 0.0001). Multiple regression analysis revealed SD score-leptin to be a significant determinant of GHBP, accounting for 64% of the variation, whereas BMI did not contribute further to explaining changes in GHBP. This suggests a physiological pathway involving both GHBP (the soluble fraction of GH receptor) and leptin. Thus, we might speculate that leptin could be the signal that induces the related nutritional changes observed in GHBP/GH receptor expression.     

Revisiting exposure: Fatal and non-fatal traffic injury risk across different populations of travelers in Wisconsin, 2001–2009

Comparing the injury risk of different travel modes requires using a travel-based measure of exposure. In this study we quantify injury risk by travel mode, age, race/ethnicity, sex, and injury severity using three different travel-based exposure measures (person-trips, person-minutes of travel, and person-miles of travel) to learn how these metrics affect the characterization of risk across populations. We used a linked database of hospital and police records to identify non-fatal injuries (2001–2009), the Fatality Analysis Reporting System for fatalities (2001–2009), and the 2001 Wisconsin Add-On to the National Household Travel Survey for exposure measures. In Wisconsin, bicyclists and pedestrians have a moderately higher injury risk compared to motor vehicle occupants (adjusting for demographic factors), but the risk is much higher when exposure is measured in distance. Although the analysis did not control for socio-economic status (a likely confounder) it showed that American Indian and Black travelers in Wisconsin face higher transportation injury risk than White travelers (adjusting for sex and travel mode), across all three measures of exposure. Working with multiple metrics to form comprehensive injury risk profiles such as this one can inform decision making about how to prioritize investments in transportation injury prevention.     

Photoconductivity and luminescence of CuInSe2 single crystals at a high level of optical excitation

The luminance-current and spectral characteristics of photoluminescence of the CuInSe₂ single crystals are studied. The superlinear portion of the excitation-intensity dependence of photoconductivity at low excitation intensities in compensated p-CuInSe₂ crystals is explained on the basis of a recombination model. The emission band that peaked at 0.98 eV in the n-CuInSe₂ photoluminescence spectrum corresponds to radiative recombination of electrons at the donor level with a depth of 0.04 eV. The maximum in the band intensity corresponds to the energy gap between the trap level and the valence band.