Histone Methylation Marks on Circulating Nucleosomes as Novel Blood-Based Biomarker in Colorectal Cancer

Circulating nucleic acids (CNAs) are under investigation as a liquid biopsy in cancer as potential non-invasive biomarkers, as stable structure in circulation nucleosomes could be valuable sources for detection of cancer-specific alterations in histone modifications. Our interest is in histone methylation marks with a focus on colorectal cancer, one of the leading cancers respective the incidence and mortality. Our previous work included the analysis of trimethylations of lysine 9 on histone 3 (H3K9me3) and of lysine 20 on histone 4 (H4K20me3) by chromatin immuno- precipitation-related PCR in circulating nucleosomes. Here we asked whether global immunologic measurement of histone marks in circulation could be a suitable approach to show their potential as biomarkers. In addition to H3K9me3 and H4K20me3 we also measured H3K27me3 in plasma samples from CRC patients (n = 63) and cancer free individuals (n = 40) by ELISA-based methylation assays. Our results show that of three marks, the amounts of H3K27me3 (p = 0.04) and H4K20me3 (p < 0.001) were significantly lower in CRC patients than in healthy controls. For H3K9me3 similar amounts were measured in both groups. Areas under the curve (AUC) in receiver operating characteristic (ROC) curves indicating the power of CRC detection were 0.620 for H3K27me3, 0.715 for H4K20me3 and 0.769 for the combination of both markers. In conclusion, findings of this preliminary study reveal the potential of blood-based detection of CRC by quantification of histone methylation marks and the additive effect of the marker combination.     

Varnish layer hardness of oriental beech (fagus orientalist L.) wood as affected by impregnation and bleaching

The impacts of impregnation and bleaching on the varnish layer hardness of Oriental beech (Fagus orientalist L.) wood were investigated. A number of bleaching combinations {[NaOH−H₂O₂], [NaOH−Ca(OH)₂−H₂O₂], [NaOH−MgSO₄−H₂O₂] [NaHSO₃−H₂C₂O₄], [NaSiO₃−H₂O₂], [KMnO₄+NaHSO₃+H₂O₃]} were applied at 18% concentration for bleaching to both impregnated and unimpregnated specimens of Oriental beech wood. Subsequently, water-based (WB) varnish was coated over the samples and the varnish layer hardness values were determined in accordance with ASTM D 4366-95. All of the chemicals used for bleaching reduced the surface hardness. However, after varnish coating, the hardness of most samples was similar to that of the varnish-coated natural (control) samples.     

A parallel algorithm with enhancements via partial objective value cuts for cluster-based wireless sensor network design

In this paper, we develop a parallel algorithm for the solution of an integrated topology control and routing problem in Wireless Sensor Networks (WSNs). After presenting a mixed-integer linear optimization formulation for the problem, for its solution, we develop an effective parallel algorithm in a Master–Worker model that incorporates three parallelization strategies, namely low-level parallelism, domain decomposition, and multiple search (both cooperative and independent) in a single Master–Worker framework.     

Surface analysis and adsorption behavior of caffeine as an environmentally friendly corrosion inhibitor at the copper/aqueous chloride solution interface

Abstract

Corrosion inhibitors based on environmentally friendly and harmless products are currently being studied and developed. The corrosion inhibition properties of caffeine (1,3,7-trimethylxanthine) on copper corrosion in aqueous chloride solution (3.5?wt.% NaCl) are analysed here using stationary and transient electrochemical methods, and a theoretical study based on density functional theory is carried out. Caffeine is a very competitive compared to the chemical inhibitors that are often used for copper protection. Electrochemical and impedance experiments reveal that the protective efficiency of caffeine reaches a value of 96% at a concentration of 10^?2?mol L^?1. Based on these results, the Langmuir model appears to be the best representation of the adsorption of caffeine onto the copper surface. Scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy and X-ray diffraction (XRD) were used to determine the surface morphology and the chemical composition of the copper surface in chloride media, in the absence and presence of caffeine. The results show the development of a mechanism of corrosion inhibition. In order to confirm the correlation between the inhibitory effect and the molecular structure of caffeine, quantum chemical parameters are used to calculate its electronic properties.     

Metamodeling live sequence charts for code generation

This article presents a metamodeling study for Live Sequence Charts (LSCs) and Message Sequence Charts (MSCs) with an emphasis on code generation. The article discusses specifically the following points: the approach to building a metamodel for MSCs and LSCs, a metamodel extension from MSC to LSC, support for model-based code generation, and finally action model and domain-specific data model integration. The metamodel is formulated in metaGME, the metamodel language for the Generic Modeling Environment.

Model‐based code generation for HLA federates

This paper addresses the problem of automated code generation for a High Level Architecture compliant federate application given its behavior model. The behavior model is a part of the architectural model of a federation that the federate can participate in. The federate behavior model is based on Live Sequence Charts, adopted as the behavioral specification formalism in the Federation Architecture Metamodel (FAMM). FAMM serves as a formal language for describing federation architectures. An objective is to help the testing of federation architecture by means of its prototype implementation early in the development lifecycle. A further objective is to help developers construct complete federate applications that are well modularized. The approach to achieve these objectives is aspect‐oriented in that the generated code, which handles the federate's interactions with a federation execution, serves as the base code, where the computation logic is to be weaved as an aspect. Copyright © 2009 John Wiley & Sons, Ltd.     

From Autonomy to Cooperative Traded Control of Humanoid Manipulation Tasks with Unreliable Communication

In this paper, we present our system design, operational procedure, testing process, field results, and lessons learned for the valve-turning task of the DARPA Robotics Challenge (DRC). We present a software framework for cooperative traded control that enables a team of operators to control a remote humanoid robot over an unreliable communication link. Our system, composed of software modules running on-board the robot and on a remote workstation, allows the operators to specify the manipulation task in a straightforward manner. In addition, we have defined an operational procedure for the operators to manage the teleoperation task, designed to improve situation awareness and expedite task completion. Our testing process, consisting of hands-on intensive testing, remote testing, and remote practice runs , demonstrates that our framework is able to perform reliably and is resilient to unreliable network conditions. We analyze our approach, field tests, and experience at the DRC Trials and discuss lessons learned which may be useful for others when designing similar systems.     

Improvements in microstructural,mechanical, and biocompatibility properties of nano-sized hydroxyapatites doped with yttrium and fluoride

Hydroxyapatite was doped with Y³⁺ (2.5, 5 and 7.5 mol%) and F⁻ (2.5 mol%) ions (2.5YFHA, 5YFHA, 7.5YFHA, respectively) to compare its structural and mechanical properties and cellular response with pure-hydroxyapatite. No second phases were observed by X-ray diffraction spectra of 2.5YFHA. Doped hydroxyapatites had F⁻ bonds in addition to OH⁻ bonds. Hydroxyapatites sintered at 900 and 1100 °C were in nano-size. 7.5YFHA sintered at 1300 °C had the highest microhardness value. 2.5YFHA sintered at 1100 °C had the highest fracture toughness value. MTT viability assays showed high cell attachments on 2.5YFHA. Cell proliferation on 2.5YFHA and 5YFHA sintered at 1100 and 1300 °C was comparable with the control after 5-day culture. The highest ALP production and calcium deposition were observed on all hydroxyapatites sintered at 1100 °C. 2.5YFHA sintered at 1100 °C can be an alternative for hydroxyapatite in orthopedic applications.