Machine learning in continuous casting of steel: a state-of-the-art survey

Journal of Intelligent Manufacturing - Continuous casting is the most important route for the production of steel today. Due to the physical, mechanical, and chemical components involved in the...     

Toward Xeno-Free Differentiation of Human Induced Pluripotent Stem Cell-Derived Small Intestinal Epithelial Cells

The small intestinal epithelium has an important role in nutrition, but also in drug absorption and metabolism. There are a few two-dimensional (2D) patient-derived induced pluripotent stem cell (iPSC)-based intestinal models enabling easy evaluation of transcellular transport. It is known that animal-derived components induce variation in the experimental outcomes. Therefore, we aimed to refine the differentiation protocol by using animal-free components. More specifically, we compared maturation of 2D-cultured iPCSs toward small intestinal epithelial cells when cultured either with or without serum, and either on Geltrex or on animal-free, recombinant laminin-based substrata. Differentiation status was characterized by qPCR, immunofluorescence imaging, and functionality assays. Our data suggest that differentiation toward definitive endoderm is more efficient without serum. Both collagen- and recombinant laminin-based coating supported differentiation of definitive endoderm, posterior definitive endoderm, and small intestinal epithelial cells from iPS-cells equally well. Small intestinal epithelial cells differentiated on recombinant laminin exhibited slightly more enterocyte specific cellular functionality than cells differentiated on Geltrex. Our data suggest that functional small intestinal epithelial cells can be generated from iPSCs in serum-free method on xeno-free substrata. This method is easily converted to an entirely xeno-free method.     

Validation of ceiling jet flows in a large corridor with vents using the CFD code JASMINE

A field model code, JASMINE, has been adopted to calculate ceiling jet flows caused by plumes from unsteady fire sources in a large corridor. The idealized fast- and medium-fire energy release rates, obeying thet ² law for fire growth, were used. The effects of vents were studied by simulating three different configurations: a centrally located vent—that is, a vent directly above the fire source; an eccentrically located vent; and no vent. The results were compared with recent large-scale experiments conducted at the Swedish National Testing and Research Institute (SP) and with results from the computer program LAVENT. Some calculated sprinkler temperatures with three different RTI values are also presented.The present JASMINE simulations agree reasonably well with measured ceiling temperatures and velocities in large-scale tests. In general, the calculated ceiling jet temperatures are slightly overestimated and the velocities slightly underestimated. Also, the layer thicknesses calculated by JASMINE are somewhat thinner than those measured. The ceiling jet theory and present LAVENT results predict very thin layer thicknesses. However, these theories are only valid when wall effects are eliminated. From the present study it can be concluded that CFD models are generally more accurate when used to predict confined and unconfined flows.     

Multivariate autoregressive modeling of autonomic cardiovascular control in neonatal lamb

The neonatal cardiovascular control system is a complicated interactive system which is under vigorous development at birth. From the measurement point of view the cardiovascular control is a closed-loop system. However, it can be examined on a beat-by-beat basis by analyzing circulatory-controlled variables with advanced signal analysis techniques. This paper proposes to use a multivariate autoregressive modeling technique in the analysis of several simultaneous physiological signals in order to examine interactions and inherent properties in the system. With the proposed multivariate autoregressive modeling technique, a signal is modeled as a linear combination of its own past and the past values of the other simultaneous signals plus a predictive error term of the model. The interactions in the system after the model identification are analyzed in frequency domain utilizing power spectrum estimates of the signals and signal contributions. The applicability of the proposed method was examined by a three-variable model between heart rate, blood pressure and respiration in the study of autonomic cardiovascular control in a chronic neonatal lamb model, in which the cardiovascular status was changed by using a beta-adrenergic autonomic nervous blockade. The study showed that the multivariate autoregressive modeling technique is a feasible technique in studying complicated interactions within the cardiovascular control system.     

Investigations of solar radiation detectors using a laboratory test facility for solar radiation meteorological instruments

A laboratory test facility for solar radiation detectors has been built and is in operation at the Aerological Station of the Swiss Meteorological Institute (SAP/SMI). This installation is conceived as a universal test bed for solar radiation exposed meteorological instruments, and consists of a commercially available solar simulator, a laser alignment system, a translation mechanism with instrument mounts, and an adjustable projection mirror. The solar simulator produces a well characterized radiation beam which can be filtered to match the terrestrial or outer space solar spectrum with an irradiance of up to one solar constant (1367 Wm⁻²). The instrument mounts and a HeNe laser beam provide a precise and easy alignment of the reference and the test instruments in the radiation beam, allowing for incident angles in the range of 15°–75°. The measurement is based on a comparison of the response of an active cavity absolute radiometer PMO6 with the signal of the test instrument.Detailed investigations of the Haenni Solar 111B type heliometer have revealed important irregularities in the sunshine threshold irradiance angular distribution. Measurements performed with and without the protection glass cover prove the exceedingly high threshold values at large declination angles to be a consequence of enhanced reflections due to the incident angle and inhomogeneities in the glass cover.Very satisfactory results have also been obtained on characterization measurements of pyranometers showing the mean values of the responsivity to be within 0.8% of the calibration values measured at the world radiation center (WRC) at Davos.     

Shared genes, shared experiences, and similarity of personality: Data from 14,288 adult Finnish co-twins.

Similarities for Extraversion (E) and Neuroticism (N) scale scores from the Eysenck Personality Inventory were evaluated in 7,144 adult twin pairs, drawn from the population-based Finnish Twin Cohort, as a function of the co-twins' genetic resemblance, gender, age, and the frequency of their social interaction with each other. To separate effects of shared genes from those of shared experience, we performed hierarchical multiple regressions of double-entry data matrices. Results establish the predictive significance of both genetic and experiential influences: Genetic effects remained significant when tested after the effects of social contact were first removed; conversely, for N scores, the effects of social contact remained significant when assessed after genetic influences were first removed. These findings establish genetic variance in major dimensions of adult personality but assign a significant role to common experience as well. The first finding constructively replicates reports by others; the second challenges the widespread assumption that shared experiences have a negligible impact on sibling similarity in adult personality. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Purification and characterisation of relevant natural and recombinant apple allergens

Apple (Malus domestica) is the most widely cultivated fruit crop in Europe and frequently causes allergic reactions with a variable degree of severity. So far, four apple allergens Mal d 1, Mal d 2, Mal d 3 and Mal d 4 have been identified. Mal d 1, a Bet v 1 related allergen, and Mal d 4, apple profilin, are sensitive to proteolytic degradation, whereas Mal d 2, a thaumatin-like protein and Mal d 3, a nonspecific lipid transfer protein, are rather stable to proteolytic processes. Mal d 1 and Mal d 4 were purified after expression in Escherichia coli expression system, while Mal d 2 and Mal d 3 were purified from apple fruit tissue. All purified proteins were subjected to detailed physicochemical characterisation to confirm their structural integrity and maintained IgE binding capacity. Detailed investigations of carbohydrate moieties of Mal d 2 demonstrated their involvement in the overall IgE binding capacity of this allergen. It was concluded that the folded structure and IgE binding capacity of all four allergens were preserved during purification.