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排序方式: 共有428条查询结果,搜索用时 0 毫秒
1.
Luiza Ghila Thomas Aga Legy Andreas Frslev Mathisen Shadab Abadpour Joao A. Paulo Hanne Scholz Helge Rder Simona Chera 《International journal of molecular sciences》2021,22(7)
The past decade revealed that cell identity changes, such as dedifferentiation or transdifferentiation, accompany the insulin-producing β-cell decay in most diabetes conditions. Mapping and controlling the mechanisms governing these processes is, thus, extremely valuable for managing the disease progression. Extracellular glucose is known to influence cell identity by impacting the redox balance. Here, we use global proteomics and pathway analysis to map the response of differentiating human pancreatic progenitors to chronically increased in vitro glucose levels. We show that exogenous high glucose levels impact different protein subsets in a concentration-dependent manner. In contrast, regardless of concentration, glucose elicits an antipodal effect on the proteome landscape, inducing both beneficial and detrimental changes in regard to achieving the desired islet cell fingerprint. Furthermore, we identified that only a subgroup of these effects and pathways are regulated by changes in redox balance. Our study highlights a complex effect of exogenous glucose on differentiating pancreas progenitors characterized by a distinct proteome signature. 相似文献
2.
Dickschat JS Bode HB Wenzel SC Müller R Schulz S 《Chembiochem : a European journal of chemical biology》2005,6(11):2023-2033
The volatiles released by agar plate cultures of two strains of the myxobacterium Stigmatella aurantiaca (strains Sg a15 and DW4/3-1) were collected in a closed-loop stripping apparatus (CLSA) and analyzed by GC-MS. Large numbers of substances from different compound classes (ketones, esters, lactones, terpenes, and sulfur and nitrogen compounds) were identified; several of them are reported from natural sources for the first time. The volatiles 2-methyltridecan-4-one (17), its isomer 3-methyltridecan-4-one (20), and the higher homologue 2-methyltetradecan-4-one (18) were identified in the extracts of both strains and were synthesized. In addition, strain Sg a15 produced 2,12-dimethyltridecan-4-one (19), 2-methyltridec-2-en-4-one (23), and a series of phenyl ketones, among them 1-phenyldecan-1-one (14) and 9-methyl-1-phenyldecan-1-one (16), whereas strain DW4/3-1 emitted traces of 10-methylundecan-2-one (21). The biosynthesis of 14 and 16 was examined in feeding experiments with deuterated precursors carried out on agar plate cultures. The leucine-derived starter unit isovalerate was shown to be incorporated into 16, as was phenylalanine-derived benzoic acid into both 14 and 16. The results point to formation both of the phenyl ketones and of the structurally related aliphatic ketones through an unusual head-to-head coupling between a starter unit such as benzoyl-CoA and a fatty acyl-CoA, followed by decarboxylation. 相似文献
3.
The thermal curing reaction of two phenolic resole resins is monitored using the fluorescence technique. The intrinsic fluorescence can be used as an indicator for cure monitoring for the first resole. As the thermal curing proceeds, the intrinsic fluorescence intensity of the resole resin decreases and exhibits a few nanometers of redshift. The fluorescence intensity of the emission maxima is correlated with the conversion measured by differential scanning spectroscopy. A linear correlation is found at three different temperatures. The intrinsic fluorescence cannot always be used for monitoring the curing process of phenolic resole resins. Thus, three intramolecular charge transfer compounds and two organic donor–π‐acceptor salts are selected and applied for the cure monitoring of the second phenolic resole resin. As the curing reaction proceeds, the fluorescence emission spectra of the probes exhibit a blue spectral shift and the intensity changes because of environmental changes. An intensity ratio method is applied in which the ratios of the low‐ to high‐intensity changes in the emission bands are used to determine the degree of the curing process. There is a smooth correlation between the intensity ratio method and the degree of cure. The method enables one to follow the changes in the polymer structure at low and intermediate degrees of the curing process (below 70%) and obtain comparable results from different types of probes during the same curing process. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 1773–1780, 2002 相似文献
4.
5.
Molly S. Bazilchuk Takashi Sumigawa Takayuki Kitamura Zhiliang Zhang Helge Kristiansen Jianying He 《Advanced Engineering Materials》2018,20(7)
6.
Helge Langseth Author Vitae Thomas D. Nielsen Author Vitae 《Pattern recognition》2009,42(11):2724-2736
One of the simplest, and yet most consistently well-performing set of classifiers is the naïve Bayes models (a special class of Bayesian network models). However, these models rely on the (naïve) assumption that all the attributes used to describe an instance are conditionally independent given the class of that instance. To relax this independence assumption, we have in previous work proposed a family of models, called latent classification models (LCMs). LCMs are defined for continuous domains and generalize the naïve Bayes model by using latent variables to model class-conditional dependencies between the attributes. In addition to providing good classification accuracy, the LCM has several appealing properties, including a relatively small parameter space making it less susceptible to over-fitting. In this paper we take a first step towards generalizing LCMs to hybrid domains, by proposing an LCM for domains with binary attributes. We present algorithms for learning the proposed model, and we describe a variational approximation-based inference procedure. Finally, we empirically compare the accuracy of the proposed model to the accuracy of other classifiers for a number of different domains, including the problem of recognizing symbols in black and white images. 相似文献
7.
Contrast-enhanced ultrasound (CEUS) has recently become an important technology for lesion detection and characterization in cancer diagnosis. CEUS is used to investigate the perfusion kinetics in tissue over time, which relates to tissue vascularization. In this paper we present a pipeline that enables interactive visual exploration and semi-automatic segmentation and classification of CEUS data.For the visual analysis of this challenging data, with characteristic noise patterns and residual movements, we propose a robust method to derive expressive enhancement measures from small spatio-temporal neighborhoods. We use this information in a staged visual analysis pipeline that leads from a more local investigation to global results such as the delineation of anatomic regions according to their perfusion properties. To make the visual exploration interactive, we have developed an accelerated framework based on the OpenCL library, that exploits modern many-cores hardware. Using our application, we were able to analyze datasets from CEUS liver examinations, being able to identify several focal liver lesions, segment and analyze them quickly and precisely, and eventually characterize them. 相似文献
8.
Several studies have documented the occurrence of high ventilation rates during cardiopulmonary resuscitation, but to date, there have been no scientific investigation of the causes of hyperventilation. The objective of the current study was to test the effects of socio-emotional stressors on lay rescuers' ventilation rate in a simulated resuscitation setting using a manikin model. A within-subjects experiment with randomized order of conditions tested lay rescuers' ventilation rate on an intubated manikin during exposure to socio-emotional stressors and during a control condition where no external stressors were present. Ventilation rates and subjective workload were significantly higher during exposure to socio-emotional stressors than during the control condition. All but one of the nine participants ventilated at a higher ventilation rate in the experimental condition. All nine participants rated the subjective workload to be higher during exposure to socio-emotional stressors. Hence, exposure to socio-emotional stressors is associated with increased ventilation rates performed by lay rescuers during simulated cardiac arrest using a manikin model. These findings might have implications for the understanding of the type of situations which hyperventilation may occur. Awareness of these situations may have implications for training of lay rescues. 相似文献
9.
Electrical characterization of individual metal‐coated polymer spheres used in isotropic conductive adhesives 下载免费PDF全文
Hoang‐Vu Nguyen Jianying He Tore Helland Helge Kristiansen Knut E. Aasmundtveit 《应用聚合物科学杂志》2016,133(31)
Isotropic conductive adhesives (ICAs) filled with metal‐coated polymer spheres (MPS) have been proposed to improve the mechanical reliability compared to conventional ICAs filled with silver flakes. The electrical properties of MPS play an important role in the electrical performance of macroscopic MPS‐based ICAs. This article deals with the electrical characterization of individual MPS using a nanoindentation‐based flat punch method, in which the resistance and the deformation of single MPS were monitored simultaneously. Four groups of silver‐coated polymer spheres (AgPS) with identical polymer cores but different silver coating thicknesses were tested. The resistance of AgPS decreases gradually with increasing deformation degree of particles, and increases when the deformation of particles is reduced. In addition, the resistance of individual AgPS is dependent on the physical properties of the silver coating, such as thickness, uniformity, and porosity. The thicker the silver coating is, the lower and more stable the resistance of AgPS is. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43764. 相似文献
10.
Junhua Zhao Shijo Nagao Gregory M Odegard Zhiliang Zhang Helge Kristiansen Jianying He 《Nanoscale research letters》2013,8(1):541
Anisotropic conductive adhesives (ACAs) are promising materials used for producing ultra-thin liquid-crystal displays. Because the mechanical response of polymer particles can have a significant impact in the performance of ACAs, understanding of this apparent size effect is of fundamental importance in the electronics industry. The objective of this research is to use a coarse-grained molecular dynamics model to verify and gain physical insight into the observed size dependence effect in polymer particles. In agreement with experimental studies, the results of this study clearly indicate that there is a strong size effect in spherical polymer particles with diameters approaching the nanometer length scale. The results of the simulations also clearly indicate that the source for the increases in modulus is the increase in relative surface energy for decreasing particle sizes. Finally, the actual contact conditions at the surface of the polymer nanoparticles are shown to be similar to those predicted using Hertz and perfectly plastic contact theory. As ACA thicknesses are reduced in response to reductions in polymer particle size, it is expected that the overall compressive stiffness of the ACA will increase, thus influencing the manufacturing process. 相似文献