Intraoperative neurological monitoring

Intraoperative neurological monitoring (INM) is the evaluation of the nervous system within the operating room (OR) environment. In this paper, the INM system is tested in a clinical setting in comparison with conventional somatosensory evoked potential (SEP) monitoring. The study results demonstrate the capability of the INM system in extracting clearer and more stable SEP signals. The high SNR of SEP signals collected in various clinical environments, including the OR, makes the INM system a robust platform for continuous monitoring. While the current use of EP monitoring is limited to intermittent analysis by a highly trained clinical neurophysiologist, the authors believe in changing this paradigm by developing continuous monitoring systems, such as the INM system, capable of automated quantitative EP analysis. This noninvasive monitoring modality will allow for a wider range of use in clinical practice. Based on volunteer and clinical patient studies, the INM monitoring system demonstrates much greater reliability and accuracy via the artifact rejection and denoising strategies. It provides more strategic filtering options for different situations under which the clinical SEP response signal could be greatly contaminated and distorted. Furthermore, the INM system offers a promising approach to signal extraction in real-time monitoring during SEP research.     

河南洛阳天津桥的演变史

河南洛阳龙门道上，有一孔古朴的石拱桥，上建小亭，为隋唐天津桥故址残迹。天津桥经历了的历代的兴衰，见证了从隋炀帝大业元年到新中国成立后的历史和发展。     

工艺因素对82B钢280 mm x325 mm铸坯凝固过程的影响

为模拟不同工艺条件下的连铸坯的凝固过程,采用有限元法模拟了82B钢连铸的凝固过程,获得280 mm×325 mm连铸坯的温度场。结果表明,随拉速从0.60 m/min增加到0.76 m/min,凝固时f_s=1处与f_s=0处皆后移,但中心凝固时间反而增加了9.8%。随过热度由15℃增加到50℃时,凝固末端f_s=1处同样后移,约后移1.1 m。根据模拟结果改进连铸工艺,当拉速从0.60 m/min增加到0.76m/min,增加轻压下工艺可改善低倍质量。     

微小隐孢子虫CP15/60基因在Hela细胞中的表达

目的 构建隐孢子虫CP15/60真核表达载体pcDNA3.1-15/60,观察其在Hela细胞中的表达。方法用Xho I和EcoR　I从pMD18-T-15/60中酶切得到CP 15/60基因,将其插入真核表达载体pCR3.1（+）的 XhoI和EcoRI位点,构建CP15/60真核表达载体pcDNA3.1-15/60,用脂质体介导法将其转染Hela细胞,并用G418加压筛选,用RT-PCR方法检测外源CP15/60基因的转录,ELISA法和间接免疫荧光法检测其活性。结果 酶切鉴定表明已成功构建了重组真核表达载体pcDNA3.1-15/60,外源CP15/60基因能在转染细胞中有效转录,经检测表达产物具有良好的生物活性。结论 已成功地构建了pcDNA3.1-15/60真核表达载体,并在Hela细胞中具有良好的表达。     

Preparation and Characterization of a Chloroperoxidase-like Catalytic Antibody

The small molecule, meso-tetra(α,α,α,α-o-phenylacetamidophenyl) porphyrin (Mr1147.0) was used as complete antigen to elicit MAb through the immunization and cell fusion techniques. The MAb 1F2 obtained was demonstrated to be very pure by MALDI/TOFMS. The subtype of MAb 1F2 is IgG2a, which has a relative molecular weight of 156,678.8 Da.No significant change in the intensity of absorption peaks in UV and CD spectra was observed over a pH range between 6 and 12. The high stability of the abzyme and the tight binding between Fe porphyrin and antibody were also demonstrated. V_max, K_m, κcat, κcat/K_m for abzyme are 5.18 × 10⁻⁸ Ms⁻¹, 1.50 × 10⁻⁸ M, 0.518 s⁻¹, 3.45 × 10⁷ M⁻¹s⁻¹, respectively. The data obtained indicate that catalytic antibody has high catalytic activity. The chloroperoxidase activity of MAb 1F2-Fe porphyrin complex is stable from 10 °C to 60 °C.     

合成丙酮酸及其酯类的反应体系热力学计算与分析

采用Yoneda基团贡献法估算了丙酮醛、丙酮酸和丙酮酸甲酯的标准生成焓Δ_f H_m~θ、标准S_m~θ以及拟合等压热容曲线。结合丙酮醛氧化酯化合成丙酮酸及丙酮酸甲酯反应的热力学数据,对丙酮醛氧化制备丙酮酸及丙酮酸酯化制备丙酮酸甲酯的摩尔焓变Δ_rH_m~θ,吉布斯自由能变Δ_r G~θ和反应平衡常数K进行了计算与分析。结果表明,丙酮醛氧化制备丙酮酸的反应为强放热反应,标准状态下Δ_r_m~θ;为—319.3 kJ/mol,丙酮酸酯化制备丙酮酸甲酯的反应为弱的放热反应,标准状态下Δ_rH_m~θ成为—61.73 kJ/mol;两个反应的吉布斯自由能变在考虑的温度范围内均为负值,且丙酮醛氧化温度为450K时,反应的焓变有最小值—345kJ/mol丙酮酸及丙酮酸甲酯的转化率较高。     

基于遗传算法的多级主压阀动态特性优化

多级主调压阀作为自动变速器液压控制系统的关键元件,其动态特性直接影响系统的快速性、稳定性和控制精度。在对阀进行结构参数优化时,先凭借经验对参数逐个调整再通过试验验证的方法,效率低且不能保证阀的性能最优。根据多级主压阀工作原理建立了多级主压阀数学模型;搭建多级主压阀Simulink仿真模型,通过分析多级主调压阀各结构参数对阀入口压力动态响应特性的影响,选取弹簧刚度、弹簧预压缩量和阻尼孔直径作为待优化变量;利用MATLAB优化工具箱中提供的遗传算法与Simulink结合对多级主调压阀的动态性能进行优化,并搭建试验台进行试验验证。仿真和试验结果表明：优化后多级主调压阀静态特性良好,负载流量突增时主压压力降低现象得到了改善,响应时间短,动态性能好。     

“规划-生态”复合型人才培养实践探索

在矿区生态修复、采煤塌陷地生态恢复等教研工作实践取得可喜成绩的同时，体会到城乡规划学科对生态学知识的需要和对"规划-生态"复合型人才的强烈渴求，基于此，培养"规划-生态"复合型人才的改革设想逐渐形成。通过创建生态学课程群，构建基于学科交叉的"规划+生态"课程模块，引入丰富的教学方法组织课程并实施教学实践；同时采用科研反哺教学的方法，将学生引入到科研课题中，通过"真题假做"和"真题真做"的方式，让学生站在科学研究的第一线，学习专业基础知识、基本技能和学科前沿知识；在此基础上，通过不断拓宽国际化教育资源，打通国际交流渠道，实现以城乡问题为导向，以生态学理论与方法为手段的跨学科人才培养模式，最终实现复合型人才培养的目标。     

Cyclic Remote Implementation of Partially Unknown Quantum Operations

We present a novel scheme of Cyclic remote implementation of partially unknown quantum operations (CRIPUQO) via a six-qubit entangled state as the quantum channel. Alice can remotely apply a partially unknown quantum operation U d (d = 0, 1) to Bob's qubit b, and Bob can remotely apply U d to Charlie's site; meanwhile, Charlie can also remotely apply U d to Alice's qubit c. It is pointed out that the CRIPUQO in the opposite direction can be perfectly achieved by changing the quantum channel. The scheme above is also generalized to systems having N observers, so that the CRIPUQO can be realized in quantum information networks with N observers in different directions by changing quantum channels.     

Minimization of states in automata theory based on finite lattice-ordered monoids

In this study, we consider a concept of complete L-fuzzy matrix, define complete lattice-valued finite automata (CLFAs) and study their properties. The definitions of statewise equivalence relations and automata equivalence relations of a CLFA are given, two algorithms are aimed at the minimization of states of a CLFA.