Combined statistical study of joint angles and ground reactionforces using component and multiple correspondence analysis

The purpose of this research is to propose a general methodology to draw gait patterns when including hip, knee and ankle angle excursions in the sagital plane and the three components of the ground reaction force. The multidimensional signal analysis procedure is divided into three main stages: 1) describing the six signals of each step through sliding averages computed for successive time windows, 2) analyzing separately the six step-by-window tables obtained for each signal through principal component analysis to reduce the excessive quantity of data, and 3) analyzing the most informative time windows of the six signals at the same time. To emphasize both linear and nonlinear relationships between the respective time windows, the signal range within a window is cut into fuzzy modalities such as, “low” “medium” and “large.” The resulting table is investigated using multiple correspondence analysis. The outcomes are gait patterns combining both time and space aspects. The factor planes obtained from multiple correspondence analysis constitute initial data models so that new data obtained from pathological gait can be directly projected onto them. Such an operation can be used to show how the rehabilitation of a particular subject evolves in relation to normal gait patterns     

Real‐time hybrid control of electrohydraulic active suspension

Electrohydraulic actuators are an attractive choice for active suspension, because these systems provide a high power‐to‐weight ratio. However, their dynamics are highly nonlinear. In addition, the use of one simple controller for both position and force is complicated, because there is a compromise between them in the case of active suspension. Most existing controllers do not efficiently fulfill the requirements, because only one state variable is considered. In this paper, we address these problems by developing a new hybrid controller for both position and force and implementing it in a real‐time test bench. Our goal is to control the vertical position of the passenger seat while tracking the force transmitted to passengers and keeping it within tolerable and comfortable limits. Therefore, the proposed controller is a combination of two controllers. Its flexible structure redirects the control signal to control the proper controlled state variable. The real‐time results of the newly designed hybrid controller are compared with those obtained using a classical proportional integral derivative controller, because this is the most widely used controller in the industry. As expected, the proposed controller demonstrates better performance in real‐time operation. Copyright © 2017 John Wiley & Sons, Ltd.     

New temperature dependent thermal conductivity data for water-based nanofluids

This paper presents effective thermal conductivity measurements of alumina/water and copper oxide/water nanofluids. The effects of particle volume fraction, temperature and particle size were investigated. Readings at ambient temperature as well as over a relatively large temperature range were made for various particle volume fractions up to 9%. Results clearly show the predicted overall effect of an increase in the effective thermal conductivity with an increase in particle volume fraction and with a decrease in particle size. Furthermore, the relative increase in thermal conductivity was found to be more important at higher temperatures. Obtained results compare favorably with certain data sets and theoretical models found in current literature.     

Anion-Exchange Chromatography at the Service of Gene Therapy: Baseline Separation of Full/Empty Adeno-Associated Virus Capsids by Screening of Conditions and Step Gradient Elution Mode

Gene therapy is opening unprecedented opportunities for novel therapeutic approaches. Based on the concept of rescuing function mutations by co-expressing the correct gene to allow biological functions to be restored, it requires the use of viral vectors to ensure the proper delivery of therapeutic genes. In this context, recombinant adeno-associated viruses (rAAV) are the most widely used vectors. Their biomanufacturing process requires the insertion of the therapeutic gene into the rAAV (full capsids). However, a percentage of rAAV that do not contain the desired gene (empty capsids), as well as partly filled capsids, might also be produced, potentially impacting the efficiency of the therapy. Therefore, the determination of the rAAV capsids’ full/empty ratio needs to be monitored to ensure consistent product quality and efficacy. Anion-exchange chromatography (AEX) can serve this need. In this contribution, thorough AEX method development, including a mobile phase, a stationary phase and gradient conditions, has highlighted its potential in supporting gene therapy. Taking advantage of the fact that viral capsids follow an “on/off” retention behavior, the application of a step gradient approach to the rAAV serotype 8 (rAAV8) allowed the unprecedented separation of rAAV8 full/empty capsids, with a resolution gain of 3.7 as compared to the resolution obtained with a fully optimized linear gradient. Finally, the developed analytical approach allowed a precise and accurate baseline separation and quantification of full and empty rAAV8 capsids, with the potential to be applied as a high-throughput quality control (QC) method.     

激光陀螺中朗缪尔流动效应的再研究

鉴于近期文献关于激光陀螺中朗缪尔流动效应的研究结果与现行理论结果的矛盾,我们对该效应利用文献［１］中环形激光器测量方法进行了再研究,得到了在放电电极间无气压差情况的实验结果。该结果进一步证明了原有理论描述的朗缪尔流动截面分布＾［２］不适用于激光陀螺。