A rapid spectroscopic method for quantification of P57 in Hoodia gordonii raw material

The feasibility of quantifying the perceived active ingredient (P57) in Hoodia gordonii raw material using Fourier transform near- and mid-infrared spectroscopy combined with chemometric techniques was investigated. The concentration of P57 (a triterpene glycoside) was determined in 146 plant samples with liquid chromatography coupled to mass spectrometry and these values were used to develop a calibration model based on the partial least squares projections to latent structures (PLS) and orthogonal projections to latent structures (O-PLS) regression algorithms. The performance of each calibration model was evaluated according to the root mean square error of prediction (RMSEP) and correlation coefficient (R²). The PLS model with 2nd derivative pre-processing predicted P57 content based on the FT-NIR spectra with the best accuracy and a correlation coefficient (R²) value of 0.9629 and the lowest RMSEP of 0.03%. These results demonstrated that FT-NIR spectroscopy can be used to rapidly quantify P57 in H. gordonii raw material with high accuracy.     

Process and technology challenges in swift-starting virtual teams

Virtual teams often face tight schedules and a need to start quickly and perform instantly. The goal of our study was to enhance understanding of the challenges faced by such teams. We used time–interaction–performance theory as the framework for following the processes and functions within virtual teams working on a systems development task. Our study provided a detailed examination of the group process, applied to virtual teams working under time pressure. The challenges faced by virtual teams in such settings showed that teams must work to enhance their effectiveness in multiple dimensions.     

Immunohistochemical evaluation after Sr-enriched biphasic ceramic implantation in rabbits femoral neck: comparison of seven different bone conditions

Strontium (Sr) has shown effectiveness for stimulating bone remodeling. Nevertheless, the exact therapeutic values are not established yet. Authors hypothesized that local application of Sr-enriched ceramics would enhance bone remodeling in constant osteoporosis of rabbits’ femoral neck bone. Seven different bone conditions were analyzed: ten healthy rabbits composed a control group, while other twenty underwent ovariectomy and were divided into three groups. Bone defect was filled with hydroxyapatite 30% (HAP) and tricalcium phosphate 70% (TCP) granules in 7 rabbits, 5% of Sr-enriched HAP/TCP granules in 7, but sham defect was left unfilled in 6 rabbits. Bone samples were obtained from operated and non-operated legs 12 weeks after surgery and analyzed by histomorphometry and immunohistochemistry (IMH). Mean trabecular bone area in control group was 0.393 mm2, in HAP/TCP – 0.226 mm2, in HAP/TCP/Sr – 0.234 mm2 and after sham surgery – 0.242 mm2. IMH revealed that HAP/TCP/Sr induced most noticeable increase of nuclear factor kappa beta 105 (NFkB 105), osteoprotegerin (OPG), osteocalcin (OC), bone morphogenetic protein 2/4 (BMP 2/4), collagen type 1α (COL-1α), interleukin 1 (IL-1) with comparison to intact leg; NFkB 105 and OPG rather than pure HAP/TCP or sham bone. We concluded that Sr-enriched biomaterials induce higher potential to improve bone regeneration than pure bioceramics in constant osteoporosis of femoral neck bone. Further studies on bigger osteoporotic animals using Sr-substituted orthopedic implants for femoral neck fixation should be performed to confirm valuable role in local treatment of osteoporotic femoral neck fractures in humans.

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Managing strategic contradictions in hybrid teams

It has become a common practice to organize work teams to include members in multiple geographic locations. In contrast to co-located teams and purely virtual teams, these ‘hybrid’ teams combine face-to-face communication with computer-mediated interaction. In this paper, we report a qualitative study of management practices in three hybrid teams in one organization. We adopt the theoretical approach of strategic contradiction, in which apparently contradictory pairs of elements can be managed by attending to their possible synergies or clarifying their distinctions so as to make balanced trade-offs over time. Our data reveal four sets of paradoxical frames in hybrid teams: remoteness–closeness, cultural uniformity–cultural diversity, rationality–emotionality, and control–empowerment. In referencing these paradoxical frames, teams engaged in three cognitive processes: (a) integrating to produce synergies between opposing tensions, (b) differentiating to clarify distinctions between opposing tensions and to balance trade-offs over time, and (c) polarizing to remove tensions between opposing elements by using one element to reduce the effects of another. Both integrating and differentiating processes were found to be instrumental to sustaining contradictions as interdependent dualities, whereas polarizing processes were found to preserve contradictions as dualisms. Our findings advance the understanding of managing strategic contradictions by showing how managers influence cognitive processes that paradoxically emphasize remoteness and closeness, cultural uniformity and cultural diversity, rationality and emotionality, and control and empowerment.     

Measuring the refractive state of an eye based on the intensity of the retinal reflex

When examining the fundus of the eye with an ophthalmoscope, the intensity of the retinal reflex depends on the refractive state of the eye. In this study, application of this phenomenon for measuring the refractive state of the eye is demonstrated. First, a calibration curve relating the intensity of the retinal reflex and the refractive state was obtained using a model eye. Next, the intensity of the retinal reflex was measured while subjects were viewing a checker-board pattern, the distance of which was varied within the range from 0.17 to 1 m. Further, the relation between the refractive state and the optical power of the stimulus could be determined based on the measured light intensity and the calibration curve. Generally, the measured refractive state increased with the optical power of the stimulus. However, blinking and fluctuations in accommodation had undesired effects on the results.     

Evaluation of the physical and antimicrobial properties of silver doped hydroxyapatite depending on the preparation method

In the present study, the effect of the preparation method on the physical and antibacterial properties of silver doped hydroxyapatite (HAp/Ag) samples was investigated. HAp/Ag with 0.1–5 % of silver was prepared using two different modified wet chemical precipitation methods. A comparison of thermal stability and thermodynamical properties indicated that the thermal stability and sintering temperature of HAp/Ag were higher than those of pure hydroxyapatite if Ca(NO₃)₂·4H₂O, AgNO₃, NH₄OH and (NH₄)₂HPO₄ were used as raw materials. Phase composition and silver release were determined by XRD and ICP-MS. The study showed that, after 50 h in simulated body fluid 0.8–1.8 % of silver of the total silver amount was released from compact HAp/Ag scaffolds, and release kinetics strongly depended on the HAp/Ag preparation method. In vitro antibacterial activity of samples from each method against the bacterial strains Staphylococcus epidermidis and Pseudomonas aeruginosa was approved. Results showed that, in the case of using Ca(OH)₂, H₃PO₄ and AgNO₃ as raw materials for HAp/Ag synthesis, higher antibacterial activity towards both bacterial strains could be obtained.     

Polycyclic aromatic hydrocarbons in meat smoked with different types of wood

The influence of the wood used for the smoking of meat on the formation of polycyclic aromatic hydrocarbons (PAH) has been studied. Ten types of wood and charcoal were used for preparation of smoked meat samples. The analytical sample preparation method implied extraction of PAH with cyclohexane, liquid–liquid extraction with N,N-dimethylformamide/water, back extraction with cyclohexane, followed by clean-up on silica solid phase extraction (SPE) column and quantification by gas chromatography–mass spectrometry. It was found that the type of wood has a significant influence on the amount of PAH in smoked meat. The samples smoked with apple-tree and alder contained the smallest PAH concentrations. The samples smoked with spruce had the highest concentrations of PAH. The difference in content of benzo[a]pyrene (from 6.04 till 35.07 μg/kg) and total PAH (from 47.94 till 470.91 μg/kg) indicates that choice of wood for smoking is one of the critical parameter to be controlled in order to diminish the contamination of food products.     

From Blood to Regenerative Tissue: How Autologous Platelet-Rich Fibrin Can Be Combined with Other Materials to Ensure Controlled Drug and Growth Factor Release

The purpose of this review is to examine the latest literature on the use of autologous platelet-rich fibrin as a drug and growth factor carrier system in maxillofacial surgery. Autologous platelet-rich fibrin (PRF) is a unique system that combines properties such as biocompatibility and biodegradability, in addition to containing growth factors and peptides that provide tissue regeneration. This opens up new horizons for the use of all beneficial ingredients in the blood sample for biomedical purposes. By itself, PRF has an unstable effect on osteogenesis: therefore, advanced approaches, including the combination of PRF with materials or drugs, are of great interest in clinics. The main advantage of drug delivery systems is that by controlling drug release, high drug concentrations locally and fewer side effects within other tissue can be achieved. This is especially important in tissues with limited blood supply, such as bone tissue compared to soft tissue. The ability of PRF to degrade naturally is considered an advantage for its use as a “warehouse” of controlled drug release systems. We are focusing on this concentrate, as it is easy to use in manipulations and can be delivered directly to the surgical site. The target audience for this review are researchers and medical doctors who are involved in the development and research of PRFs further studies. Likewise, surgeons who use PRF in their work to treat patients and who advice patients to take the medicine orally.     

Ultraviolet radiation as a non-thermal treatment for the inactivation of microorganisms in fruit juice

Fruit juices can be processed using ultraviolet (UV-C) light to reduce the number of microorganisms. The UV-C wavelength of 254 nm is used for the disinfection and has a germicidal effect against microorganisms. A novel turbulent flow system was used for the treatment of apple juice, guava-and-pineapple juice, mango nectar, strawberry nectar and two different orange and tropical juices. In comparison to heat pasteurization, juices treated with UV did not change taste and color profiles. Ultraviolet dosage levels (J L^− 1) of 0, 230, 459, 689, 918, 1 148, 1 377, 1 607 and 2 066 were applied to the different juice products in order to reduce the microbial load to acceptable levels. UV-C radiation was successfully applied to reduce the microbial load in the different single strength fruit juices and nectars but optimization is essential for each juice treated. This novel UV technology could be an alternative technology, instead of thermal treatment or application of antimicrobial compounds.

Industrial relevance

This novel UV-C system can be applied successfully to the Food Industry. UV-C can be effectively used to reduce the number of spoilage and pathogenic bacteria, as well as yeasts and moulds in different kinds of fruit juices.     

Injectable Platelet-Rich Fibrin as a Drug Carrier Increases the Antibacterial Susceptibility of Antibiotic—Clindamycin Phosphate

The aim of this study was to investigate the change in clindamycin phosphate antibacterial properties against Gram-positive bacteria using the platelet-rich fibrin as a carrier matrix, and evaluate the changes in the antibiotic within the matrix. The antibacterial properties of CLP and its combination with PRF were tested in a microdilution test against reference cultures and clinical isolates of Staphylococcus aureus (S. aureus) or Staphylococcus epidermidis (S. epidermidis). Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) analysis was done to evaluate the changes in the PRF_CLP matrix. Release kinetics of CLP was defined with ultra-performance liquid chromatography (UPLC). According to FTIR data, the use of PRF as a carrier for CLP ensured the structural changes in the CLP toward a more active form of clindamycin. A significant decrease in minimal bactericidal concentration values (from 1000 µg/mL to 62 µg/mL) against reference cultures and clinical isolates of S. aureus and S. epidermidis was observed for the CLP and PRF samples if compared to pure CLP solution. In vitro cell viability tests showed that PRF and PRF with CLP have higher cell viability than 70% after 24 h and 48 h time points. This article indicates that CLP in combination with PRF showed higher antibacterial activity against S. aureus and S. epidermidis compared to pure CLP solution. This modified PRF could be used as a novel method to increase drug delivery and efficacy, and to reduce the risk of postoperative infection.