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1.
Apolipoprotein E plays a central role in clearance of lipoprotein remnants by serving as a ligand for low-density lipoprotein and apolipoprotein E receptors. Three common alleles (apolipoprotein E(2), E(3) and E(4)) give rise to six phenotypes. Apolipoprotein E(3) is the ancestral form. Common apolipoprotein E isoforms derive from nucleotide substitutions in codons 112 and 158. Resulting cysteine-arginine substitutions cause differences in: affinities for low-density lipoprotein and apolipoprotein E receptors, low-density lipoprotein receptor activities, distribution of apolipoprotein E among lipoproteins, low-density lipoprotein formation rate, and cholesterol absorption. Accompanying changes in triglycerides, cholesterol and low-density lipoprotein may promote atherosclerosis development. Over 90% of patients with familial dysbetalipoproteinaemia have apolipoprotein E(2)/E(2). Apolipoprotein E(4) may promote atherosclerosis by its low-density lipoprotein raising effect. Establishment of apolipoprotein E isoforms may be important for patients with diabetes mellitus and several non-atherosclerotic diseases. Apolipoprotein E phenotyping exploits differences in isoelectric points. Isoelectric focusing uses gels that contain pH 4-7 ampholytes and urea. Serum is directly applied, or prepurified by delipidation, lipoprotein precipitation or dialysation. Isoelectric focusing is followed by immunofixation/protein staining. Another approach is electro- or diffusion blotting, followed by protein staining or immunological detection with anti-apolipoprotein E antibodies and an enzyme-conjugated second antibody. Apolipoprotein E genotyping demonstrates underlying point mutations. Analyses of polymerase chain reaction products are done by allele-specific oligonucleotide probes, restriction fragment length polymorphism, single-stranded conformational polymorphism, the primer-guided nucleotide incorporation assay, or denaturating gradient gel electrophoresis. Detection with primers that either or not initiate amplification is performed with the amplification refractory mutation system. Disparities between phenotyping and genotyping may derive from isoelectric focusing methods that do not adequately separate apolipoprotein E posttranslational variants, storage artifacts or faint isoelectric focusing bands. 相似文献
2.
Punt J.B. Sparreboom D. Brouwer F. Prasad R. 《Vehicular Technology, IEEE Transactions on》1998,47(4):1302-1313
Dynamic channel selection (DCS) is an algorithm for flexible resource sharing in mobile radio systems. The digital enhanced cordless telecommunications (DECT) standard implements a version of DCS based on time as well as frequency multiplexing. In this paper, mathematical models are developed to evaluate the probabilities of channel availability, desired carrier power, and the carrier-to-interference ratio (CIR) for a constant traffic load. These results can be used to compute the call setup blocking probability. The models reported in this study are based on a decentralized DCS according to the DECT standard specifications. The results show that blocking due to availability of a channel (resource blocking), not interference blocking, generally is the most important factor in overall call blocking. Furthermore, it is seen that resource blocking is sensitive to the implementation of portable sets. Also, with homogeneous static traffic, this type of DCS ran be as good as fixed channel allocation (FCA) with respect to resource blocking 相似文献
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Metabolism and biochemical effects of 3,3',4,4'-tetrachlorobiphenyl in pregnant and fetal rats 总被引:1,自引:0,他引:1
DC Morse EK Wehler M van de Pas AT de Bie PJ van Bladeren A Brouwer 《Canadian Metallurgical Quarterly》1995,95(1-2):41-56
The metabolism and distribution of a single oral dose of 25 mumol 14C-labelled 3,3',4,4'-tetrachlorobiphenyl (14C-TCB) were investigated in pregnant female Wistar rats and their fetuses. TCB was administered on day 13 of gestation and the elimination was followed for 7 days. Non-pregnant rats were treated similarly for comparison. Fecal elimination of 14C-TCB derived radioactivity was significantly lower in pregnant rats than in non-pregnant rats. The major metabolite found in adult liver and plasma, placental tissue, whole fetuses and fetal plasma was 3,3',4',5-tetrachloro-4-biphenylol (4-OH-TCB). Tissue levels (liver, abdominal fat, skin, skeletal muscle, kidney and plasma) of 14C-TCB-derived radioactivity declined by 65-85% over a 7-day period following administration in the adult animals. However, 14C-TCB-derived radioactivity accumulated more than 100-fold in the fetuses over the same time period, and GC/MS analysis revealed that the fetal accumulation in radioactivity was due primarily to 4-OH-TCB, and not the parent compound. On day 20 of gestation, concentrations of 4-OH-TCB were 14 times greater in fetal plasma than maternal plasma. Treatment with 14C-TCB significantly reduced plasma thyroxine levels by at least 28% up to 7 days after administration in non-pregnant animals and up to 4 days after administration in pregnant rats (31% decrease). By 7 days after administration plasma thyroxine levels had returned to control levels in the TCB-treated pregnant rats. However, fetal plasma thyroxine levels were significantly decreased by 35% in fetuses from 14C-TCB-treated dams 7 days after TCB administration. Hepatic microsomal ethoxyresorufin-O-deethylase (EROD) activity was significantly induced in TCB-treated dams relative to controls at 4 and 7 days after administration, while no EROD activity was detected in hepatic microsomes from control or TCB treated fetal rats at day 20 of gestation. These data suggest that hydroxylated metabolites of polychlorinated biphenyls may play a role in the development toxicity of these compounds. 相似文献
6.
Barimani Samaneh Amini Amir Chaharmahali Iman 《Journal of Computational Electronics》2021,20(3):1332-1342
Wave absorbers are considered to be fundamental building blocks for the manipulation of light. Almost all optical systems exploit absorbers to realize some functions. A highly tunable wide-band THz absorber is presented herein. Utilizing a dual-bias scheme with a single graphene layer leads to greater freedom to control the absorption response, while a conventional periodic array of graphene ribbons and a layer of graphene sheet are also exploited. Also, a circuit model representation for all the constituent parts of the proposed absorber is developed with an evolved design methodology. According to the simulation results, wide-band absorption from 3.5 to 6 THz is achieved.
相似文献7.
Iman Ahmadianfar Arvin Samadi-Koucheksaraee Omid Bozorg-Haddad 《Water Resources Management》2017,31(14):4375-4397
Deriving the optimal policies of hydropower multi-reservoir systems is a nonlinear and high-dimensional problem which makes it difficult to achieve the global or near global optimal solution. In order to optimally solve the problem effectively, development of optimization methods with the purpose of optimizing reservoir operation is indispensable as well as inevitable. This paper introduces an enhanced differential evolution (EDE) algorithm to enhance the exploration and exploitation abilities of the original differential evolution (DE) algorithm. The EDE algorithm is first applied to minimize two benchmark functions (Ackley and Shifted Schwefel). In addition, a real world two-reservoir hydropower optimization problem and a large scale benchmark problem, namely ten-reservoir problem, were considered to indicate the effectiveness of the EDE. The performance of the EDE was compared with the original DE to solve the three optimization problems. The results demonstrate that the EDE would have a powerful global ability and faster convergence than the original DE to solve the two benchmark functions. In the 10-reservoir optimization problem, the EDE proved to be much more functional to reach optimal or near optimal solution and to be effective in terms of convergence rate, standard deviation, the best, average and worst values of objective function than the original DE. Also, In the case of two-reservoir system, the best values of the objective function obtained 93.86 and 101.09 for EDE and DE respectively. Based on the results, it can be stated that the most important reason to improve the performance of the EDE algorithm is the promotion of local and global search abilities of the DE algorithm using the number of novel operators. Also, the results of these three problems corroborated the superior performance, the high efficiency and robustness of the EDE to optimize complex and large scale multi-reservoir operation problems. 相似文献
8.
The issue of exponential stability analysis of continuous‐time switched singular systems consisting of a family of stable and unstable subsystems with time‐varying delay is investigated in this paper. It is very difficult to analyze the stability of such systems because of the existence of time‐delay and unstable subsystems. In this regard, on the basis of the free‐weighting matrix approach, by constructing the new Lyapunov‐like Krasovskii functional, and using the average dwell‐time approach, delay‐dependent sufficient conditions are derived and formulated in terms of LMIs to check the exponential stability of such systems. This paper also highlights the relationship between the average dwell‐time of the switched singular time‐delay system, its stability, exponential convergence rate of differential states, and algebraic states. Finally, a numerical example is given to confirm the analytical results and illustrate the effectiveness of the proposed strategy. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
9.
Huong Thi Le Inge D Brouwer Jan Burema Khan Cong Nguyen Frans J Kok 《Nutrition journal》2006,5(1):1-8
Background
Parents of six children are facing a trial on charges of aggravated manslaughter in the care a 5 1/2 month old infant who died suddenly and neglect of their four older children for causing them to be malnourished by feeding them all an exclusively raw foods vegan diet. Both parents declined plea bargains and plan to defend themselves in court. 相似文献10.
In this paper, we consider the problem of flocking and shape‐orientation control of multi‐agent systems with inter‐agent and obstacle collision avoidance. We first consider the problem of forcing a set of autonomous agents to form a desired formation shape and orientation while avoiding inter‐agent collision and collision with convex obstacles, and following a trajectory known to only one of the agents, namely the leader of the formation. Then we build upon the solution given to this problem and solve the problem of guaranteeing obstacle collision avoidance by changing the size and the orientation of the formation. Changing the size and the orientation of the formation is helpful when the agents want to go through a narrow passage while the existing size or orientation of the formation does not allow this. We also propose collision avoidance algorithms that temporarily change the shape of the formation to avoid collision with stationary or moving nonconvex obstacles. Simulation results are presented to show the performance of the proposed control laws. 相似文献